Cytomegaloviruses in stored urine specimens

Feldman, Roger A.

doi:10.1016/s0022-3476(68)80280-x

Cited by 12 publications

(5 citation statements)

References 5 publications

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“…Cryoprotectants vary with respect to the viruses which they protect, toxicity for cell monolayers, concentration required, and ability to stabilize cell-free and cell-associated virus. Originally, 70% sorbitol (35) was used to enhance CMV recovery from frozen stocks and was subsequently applied to the preservation of virus in clinical specimens (9,19). Further attempts to improve CMV recovery included freezing separate cell-free and cell-associated virus suspensions, usually with sorbitol (25,32) and dimethyl sulfoxide (25), respectively.…”

Section: Discussionmentioning

confidence: 99%

Effect of sucrose phosphate and sorbitol on infectivity of enveloped viruses during storage

Howell¹,

Miller²

1983

J Clin Microbiol

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Cytomegalovirus and varicella-zoster virus recovery from sucrose phosphate (0.2 M SP) and 70% sorbitol (sorbitol) was compared after storage at -70, 4, and 20 degrees C over time. Recovery from 0.2 M SP was uniformly better. More tissue culture infective doses and infectious foci were recovered in cell monolayers inoculated with 0.2 M SP virus stocks as compared with viruses stored in 70% sorbitol. Although both viruses were isolated from diluted fresh stocks (10(-1) through 10(-4], freezing diluted virus suspensions generally resulted in diminished recovery. Similar stabilizing effects on respiratory syncytial virus and herpes simplex virus type 1 infectivities were observed when stocks were preserved in 0.2 M SP, as compared with 70% sorbitol. Overall, 0.2 M SP was better than 70% sorbitol for stabilizing cytomegalovirus, varicella-zoster virus, respiratory syncytial virus, and herpes simplex virus type 1 infectivities under the conditions tested.

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Section: Discussionmentioning

confidence: 99%

Effect of sucrose phosphate and sorbitol on infectivity of enveloped viruses during storage

Howell¹,

Miller²

1983

J Clin Microbiol

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“…Storage at -60° to -80°C was also satisfactory, particularly if 25-35% sorbitol was added (Weller and Hanshaw, 1962). Feldman (1968) observed that storage at -20°C resulted in complete loss of infectivity within a few days. With addition of sorbitol, such loss could be delayed for a few months.…”

Section: Stability Of Cytomegalovirus At Different Temperaturesmentioning

confidence: 93%

“…Feldman (1968) found that the titers of hCMV in samples ofurine to which only 0.2 ml of an antibiotic "cocktail" had been added remained essentially stable for 10 days at 4°C. In the only case where there was precise documentation, a 0.5-TCD 50 reduction in urine titer took place in 9 days, and 1.0 log in 16 days.…”

Section: Stability Of Cytomegalovirus In Urine or Bloodmentioning

confidence: 99%

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Cytomegalovirus

1991

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All rights reserved 90-14345 CIP No part of this book may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, electronic, mechanical, photocopying, microfilming, recording, or otherwise, without written permission from the Publisher Preface to the First EditionAlthough there are a number of excellent current reviews on one or another aspect of cytomegalovirus, the last comprehensive treatment of this subject was that of Krech et al. (197la). In view of the amazing advances in the virological, epidemiologic, and clinical knowledge of cytomegaloviruses, an up-to-date book is needed. Such a work should cover many areas of expertise and a voluminous technical literature. Each area might have been reviewed and analyzed by workers more expert than myself. However, I have embarked on the entire venture alone in order to attain unity and continuity in this book, characteristics that are not easily achieved in the more popular multiauthored works. I have tried to review the Iiterature and provide a critical summary for each area discussed. To do this, I provide as much of the primary data of the relevant works as needed and not just the qualitative conclusions. Inevitably, the flow of the narrative may be interrupted by dry facts and figures. However, such information is essential to make this a meaningful reference work. But for those not interested in such details, I have provided at what I hope are crucial points critiques and summaries.This book is not an exhaustive review of all the literature. This is probably no Ionger possible or even desirable. By selection, however, one runs the risk of having missed or ignored important papers. I am keenly aware of this, and I wish to apologize for such oversight, if that is possible.I visualize this work as being useful for physicians, scientists, and students interested in the biology of and infection by human cytomegalovirus. I feel that to understand these, the basic virology of this virus and related herpesviruses must be included. Topics that have not advanced a great deal and that are adequately described in available works, such as the anatomic pathology, have not been reviewed in detail. The emphasis has been placed on newer insights in the virology, immunology, serology, epidemiology, and especially clinical aspects of human cytomegalovirus. I wish tobring out the interactions of knowledge in these areas. Part II is a much shorter treatment of the nonhuman cytomegaloviruses, reviewed primarily from the point of view of their contribution to the understanding of the human virus. V vi Preface to the First Edition Finally, I wish to thank Drs. Charles R. Rinaldo, Jr., John A. Armstrong, and Donald N. Medearis for their critical review of the manuscript. Their suggestions were invaluable. Dr. John N. Dowling's thorough review of CMV infection in patients with malignancies was the basis of Section 13.4. I also acknowledge the indispensable and patient secretarial assistance of Ms.The remarkable medical events and advances in knowle...

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“…Simultaneous seeding of cells with an inoculum allows the laboratory to process specimens on the day they are submitted, thereby taking advantage of rapid diagnostic assays in the most economical way. Alternatively, a delay of 1 day until monolayer cell cultures are available for inoculation significantly reduces the ability to recover a labile virus such as CMV and varicella-zoster virus (2,7,8) and extends the time for reporting the laboratory results. Unexpectedly, sensitivity for detecting CMV in shell vials that were seeded and infected simultaneously was only 78% compared with inoculation of formed monolayers, and these results did not parallel those results obtained with the C. trachomatis system.…”

mentioning

confidence: 99%

Simultaneous seeding and infecting of shell vials for rapid detection of cytomegalovirus infection

Espy¹,

Smith²

1987

J Clin Microbiol

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Fifty cytomegalovirus isolates were used to infect sheli vials containing confluent MRC-5 cell monolayers and shell vials which were seeded with MRC-5 cells at thé time of inoculation. Ail 50 of the isolates were detected by immunofluorescence in shell vials containing confluent monolayers, whereas 39 (78%) of the isolates were detected in shell vials that had been seeded and inoculated shnultaneously (P < 0.001). Preformed monolayers of cells in shell vials provide the most sensitive system for the detection of cytomegalovirus infection.

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Cytomegaloviruses in stored urine specimens

Cited by 12 publications

References 5 publications

Effect of sucrose phosphate and sorbitol on infectivity of enveloped viruses during storage

Effect of sucrose phosphate and sorbitol on infectivity of enveloped viruses during storage

Cytomegalovirus

Simultaneous seeding and infecting of shell vials for rapid detection of cytomegalovirus infection

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