Cytometric analysis on cytotoxicity of curcumin on rat thymocytes: Proapoptotic and antiapoptotic actions of curcumin

Koizumi, Kazuki; Kawanai, Takuya; Hashimoto, Erika; Kanbara, Yasuhiro; Masuda, Toshiya; Kanemaru, Kaori; Okano, Yoshiro; Oyama, Yasuo

doi:10.1016/j.tiv.2011.03.010

Cited by 5 publications

(4 citation statements)

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“…These findings are in line with previous reports indicating the protective role of curcumin in lymphocytes [43,44]. On the other hand, the minimal concentration of curcumin (0.3 μM) used in this study did not affect ketamine-induced toxicity in rat thymocytes, which is also in accordance with a previous report suggesting that curcumin mainly exerts its protective effect at micromolar concentrations [24]. In addition, we demonstrated that 1 and 3 μM of curcumin effectively inhibited the ROS production and restored the MMP in ketamine-treated rat thymocytes, indicating that increased ROS production and disruption of MMP were associated with ketamine-induced cytotoxicity.…”

Section: B Asupporting

confidence: 93%

“…The final DMSO concentration never exceeded 0.5% (v/v). Cultivation with the increasing concentrations of curcumin (0.3, 1, and 3 μM) was chosen based on our earlier reports [16,23], as well as based on another study which demonstrated that 3 μM was the highest concentration of curcumin that did not induce any cytotoxic effect in rat thymocytes [24].…”

Section: Cell Culture and Treatmentsmentioning

confidence: 99%

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Modulatory effect of curcumin on ketamine-induced toxicity in rat thymocytes: Involvement of reactive oxygen species (ROS) and the phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt) pathway

Pavlović

Jović

Karan

et al. 2018

Bosn J of Basic Med Sci

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Ketamine is a widely used anesthetic in pediatric clinical practice. Previous studies have demonstrated that ketamine induces neurotoxicity and has a modulatory effect on the cells of the immune system. Here, we evaluated the potential protective effect and underlying mechanisms of natural phenolic compound curcumin against ketamine-induced toxicity in rat thymocytes. Rat thymocytes were exposed to 100 µM ketamine alone or combined with increasing concentrations of curcumin (0.3, 1, and 3 μM) for 24 hours. Cell viability was analyzed with CCK-8 assay kit. Apoptosis was analyzed using flow cytometry and propidium iodide as well as Z-VAD-FMK and Z-LEHD-FMK inhibitors. Reactive oxygen species (ROS) production and mitochondrial membrane potential [MMP] were measured by flow cytometry. Colorimetric assay with DEVD-pNA substrate was used for assessing caspase-3 activity. Involvement of phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt) signaling pathway was tested with Wortmannin inhibitor. Ketamine induced toxicity in cells, increased the number of hypodiploid cells, caspase-3 activity and ROS production, and inhibited the MMP. Co-incubation of higher concentrations of curcumin (1 and 3 μM) with ketamine markedly decreased cytotoxicity, apoptosis rate, caspase-3 activity, and ROS production in rat thymocytes, and increased the MMP. Application of Z-VAD-FMK (a pan caspase inhibitor) or Z-LEHD-FMK (caspase-9 inhibitor) with ketamine effectively attenuated the ketamine-induced apoptosis in rat thymocytes. Administration of Wortmannin (a PI3K inhibitor) with curcumin and ketamine significantly decreased the protective effect of curcumin on rat thymocytes. Our results indicate that ketamine-induced toxicity in rat thymocytes mainly occurs through the mitochondria-mediated apoptotic pathway and that the PI3K/Akt signaling pathway is involved in the anti-apoptotic effect of curcumin.

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Section: B Asupporting

confidence: 93%

Section: Cell Culture and Treatmentsmentioning

confidence: 99%

Modulatory effect of curcumin on ketamine-induced toxicity in rat thymocytes: Involvement of reactive oxygen species (ROS) and the phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt) pathway

Pavlović

Jović

Karan

et al. 2018

Bosn J of Basic Med Sci

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“…The final concentration of DMSO, applied to the cells, was less than 0.5%. Concentration of curcumin, used in our study, was chosen due to our recently published findings (Pavlovic et al 2016) and previous study results in rat thymocytes (Koizumi et al 2011), which showed that 3 μM was the lowest concentration which was not able to induce any cytotoxic actions in rat thymocytes, while MSG concentrations were adopted from previous reports (Pavlovic et al 2007;Jovic et al 2009) regarding the in vitro effect of this compound on thymocytes toxicity.…”

Section: Cell Culturementioning

confidence: 99%

The effect of curcumin and PI3K/Akt inhibitor on monosodium glutamate-induced rat thymocytes toxicity

Vučić

Ćojbašić²,

Vučić³

et al. 2018

gpb

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Monosodium glutamate (MSG), the sodium salt of glutamic acid, is widely used in modern nutrition as flavor enhancer. However, it has been shown that curcumin has ability to induce apoptosis in the cells of the immune system. In the present study, we evaluate the potential protective effects of curcumin in MSG-induced apoptosis and signaling pathway which may be involved. Rat thymocytes were treated with increased (1, 10, 50 mM) MSG concentrations and/or curcumin (3 μM). Cell apoptosis rate, reactive oxygen species (ROS) production, mitochondrial membrane potential (MMP), Bcl-2, Bax protein expression and caspase-3 activity were determined after 24 hours of incubation. Treatment with MSG resulted with increased apoptosis, ROS production and caspase-3 activity, followed with decreased MMP and Bcl-2/Bax protein ratio. Inhibition of caspase-3 and caspase-9 activity reduced cell apoptosis, indicating the involvement of mitochondrial apoptotic pathway. Co-treatment with curcumin markedly reduced apoptosis and ROS production, together with increased MMP and Bcl2/Bax protein ratio. Inhibition of PI3K/Akt signaling pathway abolished protective effect of curcumin in MSG-induced toxicity in rat thymocytes. Obtained findings suggest that curcumin may attenuate the MSG-induced apoptosis through PI3K/Akt signaling pathway which could be useful in preventing the potential deficiencies in T cell-mediated immunity.

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“…Dentre as quais uma variedade de ações terapêuticas, incluindo ações antiinflamatórias, anti-infecciosas, anti-oxidantes e anticancerígenas (Cemil et al, 2010;Wu et al, 2011;Lin et al ., 2011;Koizumi et al, 2011). Possui efeitos vasculares uma vez que leva à melhora na reperfusão pós lesão isquêmica do miocárdio, fígado, rim, cérebro e outros órgãos e sistemas (Cemil et al, 2010).…”

Section: Medula Espinhal Após Lesãounclassified

Efeitos da cúrcuma na recuperação funcional após hemissecção medular

Rocha¹

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ROCHA, V.M. Effects of curcumin on functional recovery after medular hemisection. The spinal cord injury is a condition that results in severe functional deficit. It is related to the industrial and technological development and affects mainly young adults. Despite some therapeutic trials are under investigation, there is no treatment that guarantees functional recovery. Most of those strategies for recovery involve neuroplasticity. Curcumin is a dietetic compound with known therapeutic potential, interfering on inflammatory, oxidative and immunological processes. The present study investigated the effects of the treatment with curcumin on acute phase of spinal cord hemi-section in rats. The protocol has been approved by Ethics Committee of School of Arts, Sciences and Humanities. The animals were submitted to hemisection after laminectomy and were divided in two groups: i) injured with curcumin (LC); ii) injured without treatment (LS). The following behavioral tests were performed 24h, 72h and 7 days after lesion: BBB Score, Combined Behavior Score, inclined plane and Beam Walking test aiming to evaluate medular relearning and functional recovery. The tests indicated an improvement of the sensitive-motor behavior probably by neuroprotection of tactile, pain and pressure pathways. The anti antiinflammatory and antioxidant properties of curcumin could result in functional improvement. Cellular and molecular analysis are necessary to better understand the effects could be over the conversion of some fatty acids involved on inflammation, such as arachidonic acid, reducing its action and promoting functional gain. The use of curcumin in the acute phase of spinal cord hemi-section seems to interfere positively in sensorial-motor recovery triggering neuroprotection and plasticity.

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Cytometric analysis on cytotoxicity of curcumin on rat thymocytes: Proapoptotic and antiapoptotic actions of curcumin

Cited by 5 publications

References 45 publications

Modulatory effect of curcumin on ketamine-induced toxicity in rat thymocytes: Involvement of reactive oxygen species (ROS) and the phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt) pathway

Modulatory effect of curcumin on ketamine-induced toxicity in rat thymocytes: Involvement of reactive oxygen species (ROS) and the phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt) pathway

The effect of curcumin and PI3K/Akt inhibitor on monosodium glutamate-induced rat thymocytes toxicity

Efeitos da cúrcuma na recuperação funcional após hemissecção medular

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