Cytometric diversity in marine ultraphytoplankton

Li, W. K. W.

doi:10.4319/lo.1997.42.5.0874

Cited by 60 publications

(51 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Assuming that the diversity of phytoplankton cell size and chlorophyll a fluorescence measured by flow cytometry indicate richness in physiological and genetic variations (27,28), the greatest diversity of phytoplankton was observed in the transition zone between station P2 and P4, coinciding with the highest concentrations of ultraplankton and nanoplankton 1 and 2 populations (Fig. S7).…”

Section: Resultsmentioning

confidence: 99%

Unveiling a phytoplankton hotspot at a narrow boundary between coastal and offshore waters

Ribalet

Marchetti

Hubbard

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

In terrestrial ecosystems, transitional areas between different plant communities (ecotones) are formed by steep environmental gradients and are commonly characterized by high species diversity and primary productivity, which in turn influences the foodweb structure of these regions. Whether comparable zones of elevated diversity and productivity characterize ecotones in the oceans remains poorly understood. Here we describe a previously hidden hotspot of phytoplankton diversity and productivity in a narrow but seasonally persistent transition zone at the intersection of iron-poor, nitrate-rich offshore waters and iron-rich, nitrate-poor coastal waters of the Northeast Pacific Ocean. Novel continuous measurements of phytoplankton cell abundance and composition identified a complex succession of blooms of five distinct size classes of phytoplankton populations within a 100-km-wide transition zone. The blooms appear to be fueled by natural iron enrichment of offshore communities as they are transported toward the coast. The observed succession of phytoplankton populations is likely driven by spatial gradients in iron availability or time since iron enrichment. Regardless of the underlying mechanism, the resulting communities have a strong impact on the regional biogeochemistry as evidenced by the low partial pressure of CO 2 and the nearly complete depletion of nutrients. Enhanced phytoplankton productivity and diversity associated with steep environmental gradients are expected wherever water masses with complementary nutrient compositions mix to create a region more favorable for phytoplankton growth. The ability to detect and track these important but poorly characterized marine ecotones is critical for understanding their impact on productivity and ecosystem structure in the oceans.ecotone | transition zone | iron | high-nitrate, low-chlorophyll | flow cytometry

show abstract

Section: Resultsmentioning

confidence: 99%

Unveiling a phytoplankton hotspot at a narrow boundary between coastal and offshore waters

Ribalet

Marchetti

Hubbard

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…In addition to bulk density it is thus possible to explore the heterogeneity of bacterial communities (i.e. Kell et al, 1991;Davey and Kell, 1996), and by measuring "cytometric diversity" (Li, 1997;Troussellier et al, 1999) to finally open the "bacterial black box" that dominated the ecology of planktonic microbes in the past.…”

Section: Planktonic Bacterial Heterogeneitymentioning

confidence: 99%

Using flow cytometry for counting natural planktonic bacteria and understanding the structure of planktonic bacterial communities

Gasol¹,

Giorgio²

2000

Sci. Mar.

801

717

View full text Add to dashboard Cite

SUMMARY: Flow cytometry is rapidly becoming a routine methodology in aquatic microbial ecology. The combination of simple to use bench-top flow cytometers and highly fluorescent nucleic acid stains allows fast and easy determination of microbe abundance in the plankton of lakes and oceans. The different dyes and protocols used to stain and count planktonic bacteria as well as the equipment in use are reviewed, with special attention to some of the problems encountered in daily routine practice such as fixation, staining and absolute counting. One of the main advantages of flow cytometry over epifluorescence microscopy is the ability to obtain cell-specific measurements in large numbers of cells with limited effort. We discuss how this characteristic has been used for differentiating photosynthetic from non-photosynthetic prokaryotes, for measuring bacterial cell size and nucleic acid content, and for estimating the relative activity and physiological state of each cell. We also describe how some of the flow cytometrically obtained data can be used to characterize the role of microbes on carbon cycling in the aquatic environment and we prospect the likely avenues of progress in the study of planktonic prokaryotes through the use of flow cytometry.

show abstract

“…7). The resolution of this group can be enhanced using special cluster programs (Bakker Schut et al, 1993) or other statistical methods to examine species diversity without (Li, 1997;Carr et al, 1996) or including neural networks (Balvoort et al, 1992;Boddy et al, 1994). Another way to enhance the resolution of the phytoplankton component is to stain the DNA.…”

Section: Oceanic Phytoplanktonmentioning

confidence: 99%

Application of flow cytometry in marine phytoplankton research: current applications and future perspectives

Veldhuis¹,

Kraay²

2000

Sci. Mar.

169

View full text Add to dashboard Cite

SUMMARY: A brief overview is given of current applications of flow cytometry (FCM) in marine phytoplankton research. This paper presents a selection of highlights and various technical and analytical problems we encountered during the past 10 years. In particular, the conversion of the relative values obtained in terms of size and fluorescence applying FCM to quantitative estimates of cell size, pigment concentration, genome size etc., is addressed. The introduction of DNA -cellcycle analysis made easily assessable by flow cytometry has been of great importance, allowing in situ measurement of species specific growth rates. Key questions in ecology such as factors determining the wax and wane of phytoplankton bloom can now be better answered in terms of species specific growth and mortality. Finally, flow cytometry provides detailed information of the physiological status of the individual algal cells. New staining methods enable us to distinguish between viable and non-viable cells and so will help us to elucidate the importance of "automortality" in aquatic ecosystems.

show abstract

Cytometric diversity in marine ultraphytoplankton

Cited by 60 publications

References 27 publications

Unveiling a phytoplankton hotspot at a narrow boundary between coastal and offshore waters

Unveiling a phytoplankton hotspot at a narrow boundary between coastal and offshore waters

Using flow cytometry for counting natural planktonic bacteria and understanding the structure of planktonic bacterial communities

Application of flow cytometry in marine phytoplankton research: current applications and future perspectives

Contact Info

Product

Resources

About