Cytometric evidence of cytogenetic and proliferative heterogeneity of human solid tumors

Nervi, Carlo; Badaracco, Gianna; Maisto, A.; Mauro, F.; Tirindelli-Danesi, Donatella; Starace, Giuseppe

doi:10.1002/cyto.990020507

Cited by 25 publications

(2 citation statements)

References 5 publications

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“…The first reports, describing an abnormal DNA content in lung cancer cells were published in 1982 [8,9]. Since then, many studies have been published evaluating the significance of aneuploidy in long-term survival after Non-Small Cell Lung Cancer (NSCLC) surgery.…”

Section: Discussionmentioning

confidence: 99%

Prognostic value of DNA ploidy: 5-year follow-up of patients with resectable squamous cell carcinoma (SCC) of the lung

et al. 2006

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Section: Discussionmentioning

confidence: 99%

Prognostic value of DNA ploidy: 5-year follow-up of patients with resectable squamous cell carcinoma (SCC) of the lung

et al. 2006

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“…The first publications were preoccupied mainly with colorectal carcinoma, breast carcinoma, ovarian carcinoma, and head and neck cancers [19][20][21]. Soon reports on DNA content abnormalities in lung cancer cells followed [2,22].…”

Section: Discussionmentioning

confidence: 99%

TORAKOCHIRURGIA DNA ploidy as an independent prognostic factor: 10-year results in a group of patients surgically treated for squamous cell lung cancer

Kasprzyk¹,

Dyszkiewicz²,

Roszak³

et al. 2012

kitp

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StreszczenieWstęp: Duża zmienność biologiczna raka płuca powoduje, że mimo określonego typu histologicznego i stopnia zaawansowania raka wg klasyfikacji TNM przebieg choroby jest trudny do przewidzenia. Dlatego poszukuje się molekularnych i biochemicznych czynników mających znaczenie prognostyczne i pomocnych w kwalifikacji chorych do pooperacyjnej chemioterapii. Jednym z takich czynników mogą być zaburzenia zawartości DNA w komórkach nowotworowych, choć opinie na temat rokowniczego znaczenia ploidii są wśród badaczy podzielone. Celem pracy była ocena wpływu nieprawidłowej ilości DNA w komórkach raka na wyniki odległe (10-letnie) leczenia chirurgicznego raka płaskonabłonkowego płuca. Materiał i metody: Analizie poddano grupę 110 chorych leczonych operacyjnie z powodu raka płaskonabłonkowego płuca w latach 1995-1997. W badanej grupie było 95 mężczyzn i 15 kobiet w wieku 34-77 lat (średnia 60 lat). U 52 chorych wykonano lobektomię, u 47 pneumonektomię, a u 11 inne resekcje. Większość chorych operowano w stadium IB (35 chorych), IIB (26 pacjentów) i IIIA (22 chorych). Badany materiał stanowiły preparaty parafinowe, w których metodą cytometrii przepły-wowej zbadano zawartość DNA w komórkach raka. W każdym przypadku oznaczono indeks DNA, a ilość DNA w komórkach nowotworowych zilustrowano w postaci histogramów. Analizę statystyczną przeprowadzono przy użyciu program komputerowy Statistica 10.0 i oraz StatXact 8. Wyniki: Odsetek przypadków aneuploidalnych (z nieprawidło-wą ilością DNA) wyniósł 44,5%. Nie stwierdzono korelacji mię- AbstractBackground: The biological variability of lung cancer is one of the causes of the unpredictable clinical outcome of the disease. Many investigators have devoted a lot of effort to finding molecular and biochemical prognostic factors that may prove helpful in the qualification of patients for postoperative chemotherapy. Notwithstanding various previous studies attempted to associate DNA quantification in cancer cells with the prognosis for lung cancer, there is a divergence of opinion about its value. The aim of the present study was to assess the impact of aneuploidy on late (10-year) survival in a group of patients treated surgically for squamous cell lung cancer. Material and methods: We analyzed a group of 110 patients surgically treated for squamous cell lung cancer between 1995 and 1997. The group consisted of 95 men and 15 women, aged between 34 and 77 years (the average age being 60). We performed lobectomy in 52 cases, pneumonectomy in 47 cases, and other resections in 11 patients. Most of the patients were in IB (35 cases), IIB (26 patients) and IIIA (22 cases

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Origin of multiple “primary” colon carcinomas: A retrospective flow cytometric study

Schwartz

Banner

Roseman

et al. 1986

Cancer

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Multiple, synchronous, apparently primary carcinomas of the colon are a relatively common occurrence. The DNA ploidy in 23 colon carcinomas from 10 patients was determined to see if this parameter supported common or independent origins for such synchronous lesions. Paraffin blocks of each tumor were prepared for flow cytometry, then analyzed for nuclear DNA content. In 3 of the 10 cases, the tumors within each colon differed with respect to DNA ploidy; in four cases all tumors were diploid; and in three cases both (all) tumors within each colon had identical aneuploid DNA indices. Tumors from the same colon with identical DNA histograms often had dissimilar histology. The replicate aneuploid DNA indices strongly suggest a common origin for the multiple tumors within these colons; tumors in the other groups are compatible with either single or multiple origins. These findings suggest that multiple "primary" colon carcinomas may, in some cases, arise as translumenal metastases from an initial single lesion.

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Cytometric evidence of cytogenetic and proliferative heterogeneity of human solid tumors

Cited by 25 publications

References 5 publications

Prognostic value of DNA ploidy: 5-year follow-up of patients with resectable squamous cell carcinoma (SCC) of the lung

Prognostic value of DNA ploidy: 5-year follow-up of patients with resectable squamous cell carcinoma (SCC) of the lung

TORAKOCHIRURGIA DNA ploidy as an independent prognostic factor: 10-year results in a group of patients surgically treated for squamous cell lung cancer

Origin of multiple “primary” colon carcinomas: A retrospective flow cytometric study

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