Cytometric Method for Determining the Potential Growth Rate of Phytoplankton on the Mitotic Index

Соломонова, Е. С.; Mukhanov, Vladimir

doi:10.1615/interjalgae.v17.i1.80

Cited by 2 publications

(1 citation statement)

References 12 publications

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“…Thus, our growth rates are at the lower end of those reported in the literature, most probably due to the low light intensity used in our experiments (about 40 μmol quanta m −2 s −1 ) and due to the fact, that some strains were originally isolated from cold ocean regions, namely CCMP697 and CCMP698. At 20°C and low light intensity (17.2 μmol quanta m −2 s −1 ) a growth rate of P. cordatum of 0.41 d −1 was previously reported (Solomonova and Mukhanov, 2015), similar to that of our five cultures. Here we found clear differences between the growth rates of five different cultures of P. cordatum at 20°C which may be related to genetic differences between the isolates and the composition of their associated microbial communities.…”

Section: Growth With and Without Bacteriasupporting

confidence: 92%

The microbiome of the dinoflagellate Prorocentrum cordatum in laboratory culture and its changes at higher temperatures

Sanchez-Garcia

Wang²,

Wagner‐Döbler³

2022

Front. Microbiol.

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In the ocean, phytoplankton are dependent on communities of bacteria living in the phycosphere, a hot spot of metabolic and genetic exchange. Many types of interactions between phytoplankton and phycosphere bacteria have been shown, but it is unclear if the microbial communities associated with microalgae strains in culture collections are beneficial or harmful to the host strain. Here, we studied the microbial communities associated with four strains of the dinoflagellate Prorocentrum cordatum that had been isolated from distant geographical locations and maintained in culture collection for hundreds of generations. Community composition was determined by 16S rRNA gene amplicon sequencing. The dinoflagellate host strain was the strongest parameter separating communities, while growth phase, lifestyle (particle-attached versus free-living) and temperature had only a modulating effect. Although the strains had been isolated from distant locations in the Atlantic and Pacific Ocean, 14 ASVs were shared among all strains, the most abundant ones being Gilvibacter, Marivita, uncultivated Rhodobacteraceae, Marinobacter, Hyphomonadaceae, Cupriavidus, Variovorax, and Paucibacter. Adaptation to higher temperatures resulted in specific changes in each phycosphere microbiome, including increased abundance of rare community members. We then compared the growth of the four xenic cultures to that of the axenic P. cordatum CCMP1329. At 20°C, growth of the xenic cultures was similar or slower than that of CCMP1329. At 26°C, all four xenic cultures experienced a death phase, while the axenic culture stably remained in the stationary phase. At 30°C, only two of the xenic cultures were able to grow. A shift of dinoflagellate metabolism from autotrophy to mixotrophy and competition between dinoflagellate and bacteria for limiting nutrients, including essential vitamins, may contribute to these differences in growth patterns.

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Section: Growth With and Without Bacteriasupporting

confidence: 92%

The microbiome of the dinoflagellate Prorocentrum cordatum in laboratory culture and its changes at higher temperatures

Sanchez-Garcia

Wang²,

Wagner‐Döbler³

2022

Front. Microbiol.

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Bloom-forming potentially toxic dinoflagellates Prorocentrum cordatum in marine plankton food webs

Khanaychenko¹,

Telesh²,

Skarlato³

2019

Protistology

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The article reviews the up-to-date concepts of an ambiguous status of the widely spread, bloom-forming, potentially toxic mixotrophic dinoflagellates Prorocentrum cordatum in marine planktonic food webs, with special emphasis on the ecological prerequisites of their proliferation and possible scenarios underlying the development of harmful algal blooms and their top-down control in marine coastal waters.

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Cytometric Method for Determining the Potential Growth Rate of Phytoplankton on the Mitotic Index

Cited by 2 publications

References 12 publications

The microbiome of the dinoflagellate Prorocentrum cordatum in laboratory culture and its changes at higher temperatures

The microbiome of the dinoflagellate Prorocentrum cordatum in laboratory culture and its changes at higher temperatures

Bloom-forming potentially toxic dinoflagellates Prorocentrum cordatum in marine plankton food webs

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