Cytomixis in the Microsporogenesis of Vicia faba L. (Fabaceae)

Haroun, Soliman A.; Shehri, Abdulrahman M. Al; Wadie, H. M. Al

doi:10.1508/cytologia.69.7

Cited by 71 publications

(52 citation statements)

References 17 publications

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“…Pressure difference and clumped chromatin bridges during premeiotic anaphase are the other explanations put forth by some authors. In the present case the cytomixis seems to be a natural phenomenon under direct genetic control as has been proposed by other workers (Singhal and Gill 1985, Bedi 1990, Bellucci et al 2003, Haroun et al 2004, Singhal et al 2007). …”

Section: Discussionsupporting

confidence: 80%

Effect of Cytomixis on the Pollen Size in 'Seabuckthorn' (Hippophae rhamnoides L., Elaeagnaceae)

2008

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Summary Present study reveals the occurrence of inter PMC transfer of chromatin material in the PMCs of 'Seabuckthorn' (Hippophae rhamnoides L.). The migration of chromatin is observed from early prophase up to telophase-I. In majority of the cases 2-4 PMCs are involved in chromatin transfer, however in rare occasions migration of chromatin occurs in groups. The migration of chromatin is observed to be partial as well as complete, which resulted into the formation of anucleated, hypo and hyperploid PMCs. In rare cases, direct fusion between two PMCs at AI lead to the formation of a polyploid PMC with 36 chromosomes. As a consequence of cytomixis various other associated meiotic anomalies such as chromatin stickiness at MI and late disjunction of bivalents at AI, pycnotic chromatin, bridges and laggards at anaphases and telophases are resulted. Microsporogenesis is also abnormal resulting in the formation of dyads, triads, and polyads with micronuclei. The cytomixis though do not cause much pollen sterility but it surely results in the formation of heterogenous sized fertile pollen grains. The pollen grains are classified into small, medium and large sized with medium sized pollen grains being in high frequency. The role of such fertile, especially, small and large sized pollen grains, in the origin of plants with aneuploid and polyploid chromosome number, can not be ruled out. The present meiotic count of nϭ9 is the first record for the study area.

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Section: Discussionsupporting

confidence: 80%

Effect of Cytomixis on the Pollen Size in 'Seabuckthorn' (Hippophae rhamnoides L., Elaeagnaceae)

2008

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“…Further, the frequency of PMCs with such meiotic irregularities is also noticed to be significantly higher in the accession showing high percentage of PMCs involved in cytomixis (Table 1). Similar findings on the impact of chromatin transfer and causing meiotic irregularities and consequently pollen malformation have been recorded earlier in Vicia faba (Haroun et al 2004), Caltha palustris (Kumar and Singhal 2008), Meconopsis aculeata (Singhal and Kumar 2008), Hippophae rhamnoides (Singhal et al 2008), Lychnis indica var. fimbriata (Singhal et al 2009a), Anemone rivularis (Singhal et al 2009b), Carthamus tinctorius (Sheidai et al 2009a), Alopecurus and Catbrosa (Sheidai et al 2009b), Clematis orientalis and Vicia rigidula (Kaur and Singhal 2010).…”

Section: Discussionsupporting

confidence: 87%

“…Possible causes suggested earlier included the effect of fixation (Gottschalk 1970), temperature (Narain 1976), level of ploidy (Verma et al 1984), physiological (Bahl and Tyagi 1988), stress factors coupled with genetic control (Ghanima and Talaat 2003) and direct genetic control (Bellucci et al 2003, Haroun et al 2004. In V. pallida also, the cytomixis seems to be a natural phenomenon under direct genetic control as has been proposed by other workers (Singhal and Gill 1985, Bedi 1990, Bellucci et al 2003, Haroun et al 2004, Lattoo et al 2006, Singhal et al 2007, Singhal and Kumar 2008.…”

Section: Discussionmentioning

confidence: 99%

Cytomixis and Associated Abnormalities during Male Meiosis in a New Tetraploid Cytotype of <i>Vicia pallida</i> Turcz. (Fabaceae)

Singhal

Kaur

2011

CYTOLOGIA

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Summary Cytological work on Vicia pallida Turcz. presents male meiotic studies in wild accessions gathered from the Kinnaur district of Himachal Pradesh. All 3 accessions uniformly shared the same meiotic chromosome number of n=12 in which 5 bivalents are relatively small sized as compared with the remaining 7 bivalents. This is the first report of tetraploid cytotype in the species against the earlier record of diploid cytotype (n=7) from the other region of Himalaya. Out of the 3 accessions scored presently, 1 accession showed a regular 12 bivalent formation, equal chromosome segregation during anaphases/telophases, normal tetrad formation and 99% pollen fertility with uniform sized pollen grains. The remaining 2 accessions depicted pollen mother cells (PMCs) with irregular meiotic behaviour which included the phenomenon of chromatin transfer among proximate meiocytes at different stages of meiosis, interbivalent connections and chromatin stickiness, out of plate bivalents at metaphase-I, lagging of chromosomes/laggards at anaphases, abnormal sporads (tetrads with micronuclei and polyads) and consequently some pollen sterility and pollen grains of variable sizes. The phenomenon of cytomixis which induces various meiotic abnormalities in the PMCs and consequently pollen sterility and pollen grains of heterogeneous sizes in V. pallida seems to be under direct genetic control.

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“…Cytomixis was observed only in prophase I, corresponding to 11.08% of the irregularities in leptotene/zygotene and 4.92% of those in pachytene. Ocurrence of cytomixis during prophase I, mainly in pachytene, has been reported in species of Fabaceae (Wang et al, 2002;Belluci et al, 2003;Haroun et al, 2004;Sidorchuk et al, 2004).…”

Section: Resultsmentioning

confidence: 99%

Abnormal meiotic behavior in three species of Crotalaria

Ferreira

Torres

Carvalho

et al. 2009

Pesq. agropec. bras.

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-The objective of this work was to compare the meiotic behavior and pollen grain viability of three species of Crotalaria. Slides for meiotic analysis were prepared by the air-drying technique. Pollen grain viability was measured by three staining procedures (Alexander's solution, tetrazolium chloride and fl uorescein diacetate) and in vitro germination in a sucrose solution. Eight bivalents were observed, confi rming previous reports on populations from other regions of Brazil, as well as from other countries. All species showed abnormal meiotic behavior as follows: in Crotalaria micans, cytomixis and abnormal chromosome pairing in diakinesis; in C. spectabilis, abnormal chromosome pairing in diplotene; in C. zanzibarica, shrunk nuclei in leptotene and zygotene. Pollen grains of all three species show low viability, which may be associated with the irregularities of the meiotic behavior.Index terms: Crotalaria, cytogenetics, cytomixis, meiosis, pollen grain viability. Comportamento meiótico anormal em três espécies de CrotalariaResumo -O objetivo deste trabalho foi comparar o comportamento meiótico e a viabilidade dos grãos de pólen de três espécies de Crotalaria. A análise meiótica foi realizada por meio da técnica de secagem ao ar. A viabilidade dos grãos de pólen foi avaliada por testes de coloração (corante de Alexander, cloreto de tetrazólio e diacetato de fl uoresceína) e por teste de germinação em solução de sacarose. Foram observados oito bivalentes, confi rmando relatos prévios em populações de outras regiões do Brasil e de outros países. As três espécies apresentaram comportamento meiótico irregular: em Crotalaria micans, citomixia e pareamento irregular na diacinese; em C. spectabilis, pareamento irregular no diplóteno; e em C. zanzibarica, núcleo fortemente condensado nas fases de leptóteno e zigóteno. A viabilidade dos grãos de pólen das três espécies é baixa, o que pode estar associado às irregularidades do comportamento meiótico.Termos para indexação: Crotalaria, citogenética, citomixia, meiose, viabilidade do grão de pólen.

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Cytomixis in the Microsporogenesis of Vicia faba L. (Fabaceae)

Cited by 71 publications

References 17 publications

Effect of Cytomixis on the Pollen Size in 'Seabuckthorn' (Hippophae rhamnoides L., Elaeagnaceae)

Effect of Cytomixis on the Pollen Size in 'Seabuckthorn' (Hippophae rhamnoides L., Elaeagnaceae)

Cytomixis and Associated Abnormalities during Male Meiosis in a New Tetraploid Cytotype of <i>Vicia pallida</i> Turcz. (Fabaceae)

Abnormal meiotic behavior in three species of Crotalaria

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