 Cytomorphometrical analysis of exfoliated buccal mucosal cells: Effect of smoking

Babuta, Sumit; Garg, Rohin; Mogra, Khushboo; Dagal, Neha

doi:10.5530/ami.2014.1.7

Cited by 14 publications

(10 citation statements)

References 21 publications

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“…During chronic exposure, these events can lead to preneoplastic lesions in the oral cavity and subsequently to malignancy. 13 Ramesh et al, 4 Hande and Chaudhary, 1 Goregen et al, 14 Acharya et al, 15 and Babuta et al 16 in their studies on tobacco have all reported similar findings in these parameters. In the present study, there was significant increase in the mean value of perimeter of nucleus in the study groups in comparison with the control group (p-value < 0.05).…”

supporting

confidence: 66%

Cytological Changes in Normal Oral Mucosa of Individuals with Tobacco Habits: A Cytomorphometric Study

Palakurthy

Kulkarni

Nandan

et al. 2017

The Journal of Contemporary Dental Practice

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Introduction: Oral cancer is one of the six most common cancers in the world, and globally more than 50% of head and neck cancers occur in Asia, remarkably in India. Overall, 200,000 cases of head and neck cancers occur each year in India, among which 80,000 are oral cancers. Epidemiological and clinical studies suggest a causative role of tobacco use in the evolution of oral potentially malignant and malignant disorders. Aims and objectives:The aim of the study is to evaluate independently and compare the cytological effects of smoking, tobacco chewing, and smoking in conjunction with tobacco chewing on oral mucosa by cytomorphometric analysis. Materials and methods:The study included a total of 120 individuals subdivided into four groups, each group with 30 individuals. Group I was tobacco smokers, group II tobacco chewers, and group III both tobacco smokers and chewers. Group IV comprised 30 individuals without tobacco habit. Smears were prepared from buccal mucosa of both the study and control groups using a cytobrush and stained using Papanicolaou staining. The cells were quantified using image analysis software. Results:The results of the study showed alterations in the nuclear and cellular parameters in the study groups when compared with control groups and were statistically significant (p < 0.05). Clinical significance: Simple noninvasive techniques like exfoliative cytology can be employed as a chairside technique and in mass screening programs for identification of cellular changes in oral mucosa of individuals with tobacco habits. Thereby, it can be used as an early diagnostic tool for identification of potentially cancerous and cancerous lesions. Conclusion

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supporting

confidence: 66%

Cytological Changes in Normal Oral Mucosa of Individuals with Tobacco Habits: A Cytomorphometric Study

Palakurthy

Kulkarni

Nandan

et al. 2017

The Journal of Contemporary Dental Practice

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“…[ 9 ] A 5-16.5% average increase in the NA is reported among smokers when compared to non-smokers. [ 10 11 ] In the present study, significantly altered values were observed for all the three measured parameters (NA, CA, and N/C ratio) in the group using various forms of tobacco [ Table 1 ]. These changes were directly related to the duration and frequency of habit.…”

Section: Discussionmentioning

confidence: 76%

A cytomorphometric analysis of oral mucosal changes in tobacco users

Khot¹,

Deshmane²,

Bagri-Manjarekar³

et al. 2015

J Nat Sc Biol Med

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Aim:Tobacco use is the major cause of oral cancer, which is the sixth most common form of malignancy globally. Even in the absence of clinical manifestations, early changes in the oral mucosa can be detected microscopically by exfoliative cytology. The present study aimed to study and compare the cellular changes in the oral mucosa of tobacco users using cytomorphometry.Materials and Methods:A total of 80 subjects were included: 20 without any tobacco use habits, 20 tobacco chewers, 20 smokers, and 20 mishri users. Smears were collected from each subject, fixed, and stained using Papanicolaou stain. All slides were evaluated for nuclear and cytoplasmic changes using image analysis software.Results:Statistically significant decrease in cytoplasmic area and an increase in nuclear area and nuclear-cytoplasmic ratio were observed in tobacco users.Conclusion:Tobacco in any form causes significant cellular changes, which could be the earliest indicators of developing malignant pathology. Exfoliative cytology can be used to detect such changes.

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“…Similarly, epithelial cells from tobacco/areca nut chewers have shown increase in ND and NA. 29,30 The change in ND and NA could be due to cellular adaptation needed for cell survival, proliferation, invasion and migration. 31 Although nuclear morphometry is good indicator of dysplastic change, it should be interpreted cautiously due to reason reported in previous studies.…”

Section: Discussionmentioning

confidence: 99%

A systematic scoping review on utility of cytomorphometry in the detection of dysplasia in oral potentially malignant disorders

Yuwanati

Gadbail

Gondivkar

et al. 2020

Journal of Oral Biology and Craniofacial Research

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Background: Oral exfoliative cytology is simple, non-invasive, inexpensive tools prone to subjective bias. Objective computer-based quantitative cytomorphometry technique was developed in last few decades to overcome its limitation. However, there is no consensus about the utility of cytomorphometry as a routine screening tool for early detection of dysplastic changes. Objectives: To review the utility of cytomorphometry in OPMDs in the detection of dysplasia changes. Method: A scoping review was undertaken focusing on cytomorphometric analysis in exfoliative cells for detection of oral epithelial dysplasia and cytomorphometric parameter denoting dysplasia. The systematic search was carried out in the online journal databases (Scopus, Google Scholar, PubMed, Web of Science) using keywords until December 2019 for the studies evaluated the cytomorphometry in the detection of dysplastic changes in OPMDs. Three authors independently extracted data using collection forms. Result: The search strategy found seven analytical cross-sectional studies for scoping review after eliminating 8940 out of 8947 initial results. The scoping review showed that cytomorphometry is useful tool in detecting the dysplastic changes in OPMDs. In addition, decrease in mean cellular area, increase in mean nuclear area, and decrease in mean cell diameter, increase mean nuclear diameter, increase in mean nuclear and cytoplasmic area ratio, associated with development of dysplastic changes in OPMDs. Conclusion: Cytomorphometry is promising tool, yet there is currently no-good evidence to support role of cytomorphometry in detecting the dysplastic changes in OPMDs in this setting. More research is required for refinement of cytomorphometry as a screening tool.

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 Cytomorphometrical analysis of exfoliated buccal mucosal cells: Effect of smoking

Cited by 14 publications

References 21 publications

Cytological Changes in Normal Oral Mucosa of Individuals with Tobacco Habits: A Cytomorphometric Study

Cytological Changes in Normal Oral Mucosa of Individuals with Tobacco Habits: A Cytomorphometric Study

A cytomorphometric analysis of oral mucosal changes in tobacco users

A systematic scoping review on utility of cytomorphometry in the detection of dysplasia in oral potentially malignant disorders

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