Cytonemes as Cell-Cell Channels in Human Blood Cells

Galkina, Svetlana I.; Bogdanov, Anatoly Georgievich; Davidovich, Georgy Natanovich; Sud’ina, Galina F.

doi:10.1007/978-0-387-46957-7_17

Cited by 4 publications

(6 citation statements)

References 31 publications

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“…These long-range cellular interactions were first demonstrated for embryonic (Gustafson & Wolpert, 1967) and blood cells (neutrophils) (Shao et al, 1998;Schmidtke & Diamond, 2000;Galkina et al, 2001), and more recently for nerve and other cells as summarized in numerous comprehensive reviews (Galkina et al, 2006;Gerdes, 2006;Veranic et al, 2008;Hurtig et al, 2010). These long-range cellular interactions were first demonstrated for embryonic (Gustafson & Wolpert, 1967) and blood cells (neutrophils) (Shao et al, 1998;Schmidtke & Diamond, 2000;Galkina et al, 2001), and more recently for nerve and other cells as summarized in numerous comprehensive reviews (Galkina et al, 2006;Gerdes, 2006;Veranic et al, 2008;Hurtig et al, 2010).…”

Section: Introductionmentioning

confidence: 94%

“…Recent investigations demonstrate that eukaryotic cells can communicate with their environment over distance using nanometer-wide and very long membrane tubular or tubulovesicular cellular extensions (cytonemes, membrane tethers). These long-range cellular interactions were first demonstrated for embryonic (Gustafson & Wolpert, 1967) and blood cells (neutrophils) (Shao et al, 1998;Schmidtke & Diamond, 2000;Galkina et al, 2001), and more recently for nerve and other cells as summarized in numerous comprehensive reviews (Galkina et al, 2006;Gerdes, 2006;Veranic et al, 2008;Hurtig et al, 2010).…”

Section: Introductionmentioning

confidence: 97%

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Membrane tubules attach Salmonella Typhimurium to eukaryotic cells and bacteria

Galkina

Romanova

Брагина

et al. 2010

FEMS Immunology &Amp; Medical Microbiology

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Using scanning electron microscopy techniques we measured the diameter of adhesive tubular appendages of Salmonella enterica serovar S. Typhimurium. The appendages interconnected bacteria in biofilms grown on gallstones or coverslips, or attached bacteria to host cells (human neutrophils). The tubular appendage diameter of bacteria of virulent flagellated C53 strain varied between 60 and 70 nm, thus considerably exceeding in size of flagella or pili. Nonflagellated bacteria of mutant SJW 880 strain in biofilms grown on gallstones or coverslips were also interconnected by 60-90-nm tubular appendages. Transmission electron microscopy studies of thin sections of S. Typhimurium biofilms grown on agar or coverslips revealed numerous fragments of membrane tubular and vesicular structures between bacteria of both flagellated and nonflagellated strains. The membrane structures had the same diameter as tubular appendages observed by scanning electron microscopy, indicating that tubular appendages might represent membrane tubules (tethers). Previously, we have shown that neutrophils can contact cells and bacteria over distance via membrane tubulovesicular extensions (TVE) (cytonemes). The present electron microscopy study revealed the similarities in size and behavior of bacterial tubular appendages and neutrophil TVE. Our data support the hypothesis that bacteria establish long-range adhesive interactions via membrane tubules.

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Section: Introductionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 97%

Membrane tubules attach Salmonella Typhimurium to eukaryotic cells and bacteria

Galkina

Romanova

Брагина

et al. 2010

FEMS Immunology &Amp; Medical Microbiology

View full text Add to dashboard Cite

show abstract

“…Long distance cellular adhesive interactions mediated by long tubular or tubulovesicular cellular protrusions-cytonemes, membrane tethers, nanotubes,-were firstly observed in various embryonic and blood cells 1 and later were found in nerve and other cells. 2 Study and measurement of these structures is strongly complicated by their small size, which is near the limit of resolution for optic microscopy.…”

Section: Introductionmentioning

confidence: 99%

Microbial alkaloid staurosporine induces formation of nanometer-wide membrane tubular extensions (cytonemes, membrane tethers) in human neutrophils

Galkina

Stadnichuk

Molotkovsky

et al. 2010

Cell Adhesion & Migration

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“…Formation of long and thin cellular protrusions which connect cells to substrata and to other cells is a wide‐spread phenomenon, that is observed in various embryonic cells (Galkina et al , 2006a), blood cells (Shao et al , 1998; Schmidtke & Diamond, 2000; Galkina et al , 2001, 2005, 2006b; Raghunathan et al , 2001; Marcus & Hochmuth, 2002; Park et al , 2002; Gupta & DeFranco, 2003; Onfelt et al , 2004; Ramachandran et al , 2004; Watkins & Salter, 2005), nerve cells (Rustom et al , 2004) and astrocytes (Gimsa et al , 2007). Because of the resolution limitations of optic microscopy, these structures have not been studied extensively.…”

Section: Introductionmentioning

confidence: 99%

“…It is one of the most reliable inducers of cytoneme formation in neutrophils, capable of evoking TVE formation in >90% of neutrophils (Galkina et al , 2001). Inhibition of glucose metabolism or blocking of ATPase (vacuolar type) also initiates formation of TVE on the neutrophil cell bodies (Galkina et al , 2001, 2006a, b).…”

Section: Introductionmentioning

confidence: 99%

Nitric oxide-induced membrane tubulovesicular extensions (cytonemes) of human neutrophils catch and holdSalmonella entericaserovar Typhimurium at a distance from the cell surface

Galkina

Romanova

Stadnichuk

et al. 2009

FEMS Immunol Med Microbiol

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Nitric oxide (NO) plays an important role in host defense against bacterial infections such as salmonellosis. NO and 4-bromophenacyl bromide (BPB) induce the formation of long tubulovesicular extensions (TVE, cytonemes, membrane tethers) from human neutrophils. These TVE serve as cellular sensory and adhesive organelles. In the present study, we demonstrated that in the presence of the NO donor, diethylamine NONOate or BPB human neutrophils bound and aggregated Salmonella enterica serovar Typhimurium bacteria extracellularly by TVE. In contrast, inhibition of NO-synthase activity by N(omega)-nitro-L-arginine methyl ester stimulated neutrophil phagocytosis (ingestion) of bacteria. Neutrophil TVE consisted of membrane-covered cytoplasm as was shown by the fluorescent cytoplasmic dye 2',7'-bis(2carboxyethyl)-5,(6)-carboxyfluorescein, and the fluorescent lipid, BODIPY-labeled sulfatide. Disruption and shedding of TVE were accompanied by the appearance of specific invaginations (porosomes) on neutrophil cell bodies. These invaginations corresponded to the variations in diameter of TVE (160-240 nm). We hypothesized that TVE represented protrusions of neutrophil exocytotic trafficking through special structures on the neutrophil surface. In conclusion, we propose a novel mechanism by which NO-induced TVE formation enables neutrophils to bind and aggregate bacteria at a distance.

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Cytonemes as Cell-Cell Channels in Human Blood Cells

Cited by 4 publications

References 31 publications

Membrane tubules attach Salmonella Typhimurium to eukaryotic cells and bacteria

Membrane tubules attach Salmonella Typhimurium to eukaryotic cells and bacteria

Microbial alkaloid staurosporine induces formation of nanometer-wide membrane tubular extensions (cytonemes, membrane tethers) in human neutrophils

Nitric oxide-induced membrane tubulovesicular extensions (cytonemes) of human neutrophils catch and holdSalmonella entericaserovar Typhimurium at a distance from the cell surface

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