Cytoophidia Maintain the Integrity of Drosophila Follicle Epithelium

Wang, Qiao-Qi; You, Dong-Dong; Liu, Ji‐Long

doi:10.3390/ijms232315282

Cited by 7 publications

(5 citation statements)

References 35 publications

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“…Cytoophidia can be categorized into two distinct classes: micro-cytoophidia, measuring approximately 1-4 μm in length, and macrocytoophidia, which can extend up to 30-40 μm [6]. Functionally, cytoophidia serve as scaffolds [20][21][22] and effectively prolong the half-life of CTPS [23]. Considering that CTPS is the rate-limiting enzyme, it is crucial to gain a comprehensive understanding of its regulatory mechanism.…”

Section: / 42mentioning

confidence: 99%

Connecting Hippo pathway and cytoophidia inDrosophilaposterior follicle cells

Weng,

Zhang,

Liu

2023

Preprint

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CTP synthase (CTPS), the rate-limiting enzyme inde novosynthesis of CTP, assembles into filamentous structures termed cytoophidia. Here we study the impact of Hippo pathway on the posterior follicle cells (PFCs) inDrosophilaegg chambers. We find that the inactivation of Hippo pathway correlates with a reduction in cytoophidium length and number within PFCs. During the overexpression of CTPS, the presence of Hippo mutations also reduces the length of cytoophidia in PFCs. In addition, we observe that knocking down CTPS mitigateshpo(Hippo)-associated over-proliferation. In summary, our results suggest a connection between the Hippo pathway and the nucleotide biosynthesis enzyme CTPS in PFCs.

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Section: / 42mentioning

confidence: 99%

Connecting Hippo pathway and cytoophidia inDrosophilaposterior follicle cells

Weng,

Zhang,

Liu

2023

Preprint

Self Cite

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show abstract

“…Cytoophidia can be categorized into two distinct classes: micro-cytoophidia, measuring approximately 1-4 µm in length, and macro-cytoophidia, which can extend to up to 30-40 µm [6]. Functionally, cytoophidia serve as scaffolds [20][21][22] and effectively prolong the half-life of CTPS [23]. Considering that CTPS is a rate-limiting enzyme, it is crucial to gain a comprehensive understanding of its regulatory mechanism.…”

Section: Introductionmentioning

confidence: 99%

Connecting Hippo Pathway and Cytoophidia in Drosophila Posterior Follicle Cells

Weng,

Zhang,

Liu

2024

IJMS

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CTP synthase (CTPS), the rate-limiting enzyme in the de novo synthesis of CTP, assembles into a filamentous structure termed the cytoophidium. The Hippo pathway regulates cell proliferation and apoptosis. The relationship of the nucleotide metabolism with the Hippo pathway is little known. Here, we study the impact of the Hippo pathway on the cytoophidium in Drosophila melanogaster posterior follicle cells (PFCs). We find that the inactivation of the Hippo pathway correlates with reduced cytoophidium length and number within PFCs. During the overexpression of CTPS, the presence of Hippo mutations also reduces the length of cytoophidia in PFCs. In addition, we observe that knocking down CTPS mitigates hpo (Hippo)-associated over-proliferation. In summary, our results suggest that there is a connection between the Hippo pathway and the nucleotide biosynthesis enzyme CTPS in PFCs.

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“…However, as early as the 1970s efforts have been made to develop ex-vivo culture protocols for late-stage egg chambers (Petri et al, 1979; Gutzeit and Koppa, 1982). Optimization of late-stage cultures allows survival of stage 10-14 egg chambers for up to 10 hours (Peters and Berg, 2016; Parsons et al, 2023; Zhang and Liu, 2024). Stage 9 and earlier egg chambers have proven to be more difficult to culture and require the addition of insulin to the media to achieve survival for 4-5 hours (Prasad and Montell, 2007).…”

Section: Introductionmentioning

confidence: 99%

Bellymount-Pulsed Tracking: A Novel Approach for Real-Time In vivo Imaging of Drosophila Abdominal Tissues

Balachandra,

Amodeo

2024

Preprint

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Quantitative live imaging is a valuable tool that offers insights into cellular dynamics. However, many fundamental biological processes are incompatible with current live imaging modalities. Drosophila oogenesis is a well-studied system that has provided molecular insights into a range of cellular and developmental processes. The length of the oogenesis coupled with the requirement for inputs from multiple tissues has made long-term culture challenging. Here, we have developed Bellymount-Pulsed Tracking (Bellymount-PT), which allows continuous, non-invasive live imaging of Drosophila oogenesis inside the female abdomen for up to 16 hours. Bellymount-PT improves upon the existing Bellymount technique by adding pulsed anesthesia with periods of feeding that support the long-term survival of females during imaging. Using Bellymount-PT we measure key events of oogenesis including egg chamber growth, yolk uptake, and transfer of specific proteins to the oocyte during nurse cell dumping with high spatiotemporal precision within the abdomen of a live female.

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Cytoophidia Maintain the Integrity of Drosophila Follicle Epithelium

Cited by 7 publications

References 35 publications

Connecting Hippo pathway and cytoophidia inDrosophilaposterior follicle cells

Connecting Hippo pathway and cytoophidia inDrosophilaposterior follicle cells

Connecting Hippo Pathway and Cytoophidia in Drosophila Posterior Follicle Cells

Bellymount-Pulsed Tracking: A Novel Approach for Real-Time In vivo Imaging of Drosophila Abdominal Tissues

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