Cytopathic and Non-cytopathic Biotypes of Border Disease Virus Induce Polypeptides of Different Molecular Weight with Common Antigenic Determinants

Dutia, Bernadette M.; Entrican, Gary; Nettleton, P. F.

doi:10.1099/0022-1317-71-5-1227

Cited by 28 publications

(11 citation statements)

References 25 publications

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“…This confirms the results obtained in RIP performed with lysates from cells infected with the Moredun cp and ncp BD virus strains (Dutia et al, 1990). The Mr values of the p125 proteins from cpBVD virus ranged between about 114K (Oregon) and about 128K (NADL).…”

Section: Discussionsupporting

confidence: 87%

Heterogeneous expression of the non-structural protein p80/p125 in cells infected with different pestiviruses

Greiser‐Wilke

Dittmar

Ließ

et al. 1992

Journal of General Virology

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In order to analyse the expression of the non-structural (ns) protein p80/p125 in cells infected with different pestiviruses at the protein level, radioimmunoprecipitations with the pestivirus-specific monoclonal antibody (MAb) BVD/C16 were performed. Cell lysates infected with cytopathic (cp) and non-cytopathic (ncp) bovine viral diarrhoea (BVD) virus strains and isolates, and with hog cholera (HC) virus strains were analysed. From cpBVD virus-infected cells, the MAb precipitated one or more proteins corresponding to ns p125, displaying a marked size heterogeneity. In contrast, the lower Mr ns p80 proteins from all cpBVD virus strains and isolates analysed had identical electrophoretic motility. The ncpBVD virus strains displayed either one single band or a doublet of the p 125 protein and no p80 cleavage products. The p125 proteins precipitated from HC virus-infected cells showed no size heterogeneity. The possibility is discussed that multiple recombination events, including both insertions or deletions in the genomes of ncpBVD viruses, may lead to the heterogeneous expression of the ns p125 in cpBVD virus populations.

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Section: Discussionsupporting

confidence: 87%

Heterogeneous expression of the non-structural protein p80/p125 in cells infected with different pestiviruses

Greiser‐Wilke

Dittmar

Ließ

et al. 1992

Journal of General Virology

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“…The alveolar cell suspensions were phenotypically analysed prior to adherence on the plates with a panel of MoAbs specific for ovine cell surface molecules. These were 36F (CD2) [13], VPM65 (CD14) [14], CC21 (CD21) [15], SBU LCA1.28 (CD45) [16], 86D (gd TCR) [17] and the control antibody VPM49 (specific for an antigen of ruminant pestiviruses) [18]. Prior to staining, the cells were incubated for 15 min with heat-inactivated normal rabbit serum diluted 1:50 in HBSS containing 2% FBS and 0·01% sodium azide.…”

Section: Characterization Of Lavage Cellsmentioning

confidence: 99%

Cytokine release by ovine macrophages following infection withChlamydia psittaci

Entrican

Wilkie

McWaters

et al. 1999

Clinical and Experimental Immunology

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Chlamydia psittaci is an obligate intracellular pathogen that causes abortion in both sheep and humans. The disease in sheep (but not humans) is characterized by a long-term persistent phase that appears to be under the control of interferon-gamma. However, nothing is known about cytokine induction that precedes the persistent phase in sheep. Primary alveolar lavage cells recovered from normal adult sheep were used to study cytokine production in the first 72 h of infection with C. psittaci. These cells were phenotypically characteristic of macrophages, being adherent, phagocytic, CD14+ and staining positive for non-specific esterase. In vitro infection of the macrophages with C. psittaci resulted in the release of IL-1beta, IL-8 and granulocyte-macrophage colony-stimulating factor (GM-CSF) as measured by ovine-specific ELISAs. Heat-treated chlamydiae (1 h at 65 degrees C) did not induce the release of IL-1beta, but the release of IL-8 was similar to that induced by untreated organisms. The cells from different sheep varied most notably in their patterns of GM-CSF release in response to heat-treated and untreated organisms.

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“…In cells infected ~fith CP pesti~firuses not only p125, but two proteins derived from p125, namely p54 and p80, are also produced. At the present time, expression of p80 is the only apparent molecular marker of cytopathogenici~ (Pocock et al, 1987;Dutia et al, 1990;Meyers et aL, 1991). The p80 protein is important because it is a highly conserved and imnaunogenic protein, with all infected animals having high levels of antibody against it.…”

Section: Cytopathogenicitymentioning

confidence: 99%

Ruminant pestiviruses

Nettleton

Entrican

1995

British Veterinary Journal

Self Cite

147

106

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The ruminant pestiviruses, bovine virus diarrhoea virus (BVDV) and border disease virus (BDV) are highly successful and important pathogens which infect ruminant species worldwide. Although the serological relationships among ruminant pestiviruses require further clarification, there is growing evidence for two antigenic groups, one of which predominates in cattle and one in sheep. The success of pestiviruses stems from the ability of the non-cytopathic (NCP) biotype of the virus to cross the placenta and establish a persistent infection (PI) in the developing foetus. This biotype should be regarded as the 'normal' biotype with the cytopathic (CP) biotype being an abnormal virus that is usually isolated only from PI animals dying from mucosal disease. Recent molecular evidence points to CP viruses arising from their NCP counterparts by recombination events that include the insertion of host RNA and/or the duplication of viral RNA sequences. However, the biological mechanism through which CP viruses kill cells remains unknown. Virtually all CP and NCP viruses cause only mild, transient clinical symptoms in healthy adult animals and stimulate a protective immune response. Despite the urgent requirement for a safe, effective vaccine, there is still no commercial vaccine that has been shown to immunize dams so that foetal infection is prevented. In the absence of an effective vaccine, reliable diagnostic techniques are essential to implement effective control measures. There is now a range of monoclonal antibody-based enzyme-linked immunosorbent assays for identifying PI or convalescent animals. These tests are specific, rapid, sensitive and reliable but may themselves become redundant as they are superceded by ever-increasing molecular biology-based techniques.

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Cytopathic and Non-cytopathic Biotypes of Border Disease Virus Induce Polypeptides of Different Molecular Weight with Common Antigenic Determinants

Cited by 28 publications

References 25 publications

Heterogeneous expression of the non-structural protein p80/p125 in cells infected with different pestiviruses

Heterogeneous expression of the non-structural protein p80/p125 in cells infected with different pestiviruses

Cytokine release by ovine macrophages following infection withChlamydia psittaci

Ruminant pestiviruses

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