Cytopathogenesis of Naegleria fowleri Thai strains for cultured human neuroblastoma cells

Tiewcharoen, Supathra; Malainual, Nat; Junnu, Virach; Chetanachan, Pruksawan; Rabablert, Jundee

doi:10.1007/s00436-007-0866-3

Cited by 7 publications

(7 citation statements)

References 7 publications

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“…4c,b demonstrated the active pseudopods around Vero cells. Therefore, The virulent strain of N. fowleri could be distinguished from the environmental strain by the rapid initial rate of destruction on SK-N-MC cells (Tiewcharoen et al 2008). …”

Section: Resultsmentioning

confidence: 99%

“…Models using neuron cells should provide target that represent the neuropathogenesis in brain tissue. Nowadays, the susceptibility of human neuroblastoma (SK-N-MC) cells in co-culture of N. fowleri has proved useful to study the cytopathic effect of N. fowleri (Tiewcharoen et al 2008). Nevertheless, the comparison of destructive mechanism of SK-N-MC and Vero cell damage are not known in N. fowleri.…”

Section: Introductionmentioning

confidence: 99%

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Scanning electron microscopic study of human neuroblastoma cells affected with Naegleria fowleri Thai strains

et al. 2008

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In order to understand the pathogenesis of Naegleria fowleri in primary amoebic meningoencephalitis, the human neuroblastoma (SK-N-MC) and African green monkey kidney (Vero) cells were studied in vitro. Amoeba suspension in cell-culture medium was added to the confluent monolayer of SK-N-MC and Vero cells. The cytopathic activity of N. fowleri trophozoites in co-culture system was elucidated by scanning electron microscope at 3, 6, 9, 12, and 24 h. Two strains of N. fowleri displayed well-organized vigorous pseudopods in Nelson's medium at 37 degrees C. In co-culture, the target monolayer cells were damaged by two mechanisms, phagocytosis by vigorous pseudopods and engulfment by sucker-like apparatus. N. fowleri trophozoites produced amoebostomes only in co-culture with SK-N-MC cells. In contrast, we could not find such apparatus in the co-culture with Vero cells. The complete destruction time (100%) at 1:1 amoeba/cells ratio of SK-N-MC cells (1 day) was shorter than the Vero cells (12 days). In conclusion, SK-N-MC cells were confirmed to be a target model for studying neuropathogenesis of primary amoebic meningoencephalitis.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Scanning electron microscopic study of human neuroblastoma cells affected with Naegleria fowleri Thai strains

et al. 2008

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“…The infection by N. fowleri occurs when Naegleria-containing water enters the nose and subsequently migrates to the CNS along with the olfactory bulbs. N. fowleri invades the CNS of the host through a contact-dependent process and secretion of soluble cytolytic factors [5][6][7][8]. Once N. fowleri reaches the brain, it elicits a significant immune response by activating the innate immune system.…”

Section: Introductionmentioning

confidence: 99%

Extracellular Vesicles from Naegleria fowleri Induce IL-8 Response in THP-1 Macrophage

et al. 2022

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Extracellular vesicles (EVs) released from pathogenic protozoans play crucial roles in host–parasite communication and disease pathogenesis. Naegleria fowleri is a free-living protozoan causing primary amoebic meningoencephalitis, a fatal disease in the central nervous system. This study aims to explore the roles of N. fowleri-derived EVs (Nf-EVs) in host–pathogen interactions using the THP-1 cell line as a model. The Nf-EVs were isolated from the N. fowleri trophozoite culture supernatant using sequential centrifugation and characterized by nanoparticle tracking analysis and transmission electron microscopy. The functional roles of Nf-EVs in the apoptosis and immune response induction of THP-1 monocytes and macrophages were examined by flow cytometry, quantitative PCR, and ELISA. Results showed that Nf-EVs displayed vesicles with bilayer membrane structure approximately 130–170 nm in diameter. The Nf-EVs can be internalized by macrophages and induce macrophage responses by induction of the expression of costimulatory molecules CD80, CD86, HLA-DR, and CD169 and the production of cytokine IL-8. However, Nf-EVs did not affect the apoptosis of macrophages. These findings illustrate the potential role of Nf-EVs in mediating the host immune cell activation and disease pathogenesis.

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“…The trophozoite pellet was harvested and kept at 4˚C for 10 min, then centrifuged at 5,000 × g for 10 min. The pellets were resuspended in 5 ml fresh culture medium and the number of viable cells in each preparation was determined by using 0.4% trypan blue exclusion method [17].…”

Section: Amoebae Cultivationmentioning

confidence: 99%

Activity of chlorpromazine on nfa1 and Mp2CL5 genes of Naegleria fowleri trophozoites

Tiewcharoen¹,

Rabablert²,

Chetannachan³

et al. 2011

Health

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Amoeba treatment of patients suffering from pri-mary amoebic meningoencephalitis caused by Naegleria fowleri has not been successful. Dam-aged morphology and effect on genes of N. fowleri as the result of its initial interaction with drug may provide clue to the success of treatment. In this study, we investigated the activity of chlorpromazine compared with amphotericin B and voriconazole against N.fowleri Khon Kaen strain using cell based assay and molecular techniques. Scanning electron and light micro-graph showed the drug interaction of treated amoebae with 0.098 ug/ml chlorpromazine was faster than 0.002 ug/ml amphotericin B and 12.5 ug/ml of voriconazole. The morphological cha-racteristics of treated amoebae with Gomori’s trichrome stain correlated to the scanning elec-tron microscope study. The effect of drugs to nfa1 and Mp2CL5 genes of treated amoebae found that at 120 min post exposure, chlorpromazine, voriconazole inhibited both genes except amphotericin B. Most of drug inhibited nfa1 except fluconazole. The results evaluated that chlorpromazine was higher potency and rapidly activity than amphotericin B and voriconazole against N. fowleri trophozoites

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Cytopathogenesis of Naegleria fowleri Thai strains for cultured human neuroblastoma cells

Cited by 7 publications

References 7 publications

Scanning electron microscopic study of human neuroblastoma cells affected with Naegleria fowleri Thai strains

Scanning electron microscopic study of human neuroblastoma cells affected with Naegleria fowleri Thai strains

Extracellular Vesicles from Naegleria fowleri Induce IL-8 Response in THP-1 Macrophage

Activity of chlorpromazine on nfa1 and Mp2CL5 genes of Naegleria fowleri trophozoites

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