Cytophotometric Determination of DNA in Minimal Invasive Cancer of the Human Cervix uteri

Herzog, R. E.; Ciprian, Claudio

doi:10.1159/000299419

Cited by 4 publications

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“…In our patients with minimal invasive or early adenocarcinoma the increased values of DNA, DNA distribution, S-cells and the S/G2-index, and the decreased G2-cells indicate a higher proliferation rate of these tumor cells and demonstrate the transition into a truly invasive cancer. That agrees with the theory of tumor development of Böhm and Sandritter (1976) and our own investigations in cervical carcinoma [Herzog and Ciprian, 1983] and breast carcinoma [Herzog, 1979]. The decreased proliferation rate in invasive cancers may represent a resting stage of carcinoma, a higher proliferation rate is found again in some cases with lymphangiosis carcinomatosa (unpublished data).…”

Section: Discussionsupporting

confidence: 90%

Histographic DNA Pattern as an Aid for Diagnosis and Therapy of Minimal Invasive Endometrial Cancer

Herzog¹,

Pellicer²

1984

Oncol Res Treat

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The atypical adenomatous hyperplasia of the endometrium may be considered as carcinoma in situ. The histologic diagnosis from curettings of passages to minimal invasive or occult carcinomas of the corpus uteri may be difficult. Also the determination of DNA in the cell nuclei and its histographic evaluation do not permit to differentiate between atypical hyperplasias and invasive adenocarcinomas. Only the early or minimal invasive carcinoma shows a higher rate of proliferation, assuming a stepwise development of carcinoma in which phases of activity and retirement are changing. In these cases the DNA photometry may support the histologic diagnosis of invasive growth and facilitate the choice of therapy.

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Section: Discussionsupporting

confidence: 90%

Histographic DNA Pattern as an Aid for Diagnosis and Therapy of Minimal Invasive Endometrial Cancer

Herzog¹,

Pellicer²

1984

Oncol Res Treat

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Semi-automated diagnosis of cervical intra-epithelial neoplasia grade 2 by the measurement of acid labile DNA in cytologically normal nucle

Sincock¹

1986

Cancer

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Cytologically normal and abnormal nuclei in cervical smears from patients with cervical intra-epithelial neoplasia (CIN) grade 2 were assessed for their acid labile DNA content using the Feulgen technique. Semi-automated measurements were performed blind using a Vickers M86 microdensitometer (Vickers Instruments, Ltd., York, UK) interfaced to a Research Machines 380Z microcomputer (Research Machines, Ltd., Oxford, UK). They showed that significantly elevated levels of rapidly hydrolyzed DNA were present in both abnormal and normal looking cell fractions. Although the levels were lower in the nuclei of normal appearance, they were still significant over the early part of the hydrolysis profile. It is suggested that because these nuclei represent a more accessible fraction in CIN grade 2 smears they could prove extremely helpful in the cytodiagnosis of cervical neoplasms.

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A model for quantitative follow‐up studies of cervical lesions

Zeppa

Poel

Boon

et al. 1992

Diagnostic Cytopathology

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A model for follow-up studies of cervical lesions is described in which the specimen were taken in a noninvasive way using Cytobrushes and in which automatic measurement of the abnormal nuclei in the sampled epithelial fragments was possible because 2-microns thin Feulgen-stained plastic sections were prepared. The planimetric parameters AREA, PERIMETER, FORM PE, and FORM ELL, and the densitometric parameters optical density (OD) and integrated optical density (IOD) were assessed. The mean total volume and the mean total DNA were calculated using stereological methods. The moderate dysplasias differed from the carcinoma in situs for all the densitometric parameters except for OD, and from the invasive carcinomas for IOD and 5cER. In the moderate-dysplasia group, there were three types of DNA histograms: a highly abnormal type resembling the histograms of the carcinoma group, a normal type, and an intermediate type. Changes in DNA histograms can be established during follow-up studies of dysplasias without having disturbed the lesion due to the efficient and elegant noninvasive sampling method that was not used in earlier quantitative studies.

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Cytophotometric Determination of DNA in Minimal Invasive Cancer of the Human Cervix uteri

Cited by 4 publications

References 1 publication

Histographic DNA Pattern as an Aid for Diagnosis and Therapy of Minimal Invasive Endometrial Cancer

Histographic DNA Pattern as an Aid for Diagnosis and Therapy of Minimal Invasive Endometrial Cancer

Semi-automated diagnosis of cervical intra-epithelial neoplasia grade 2 by the measurement of acid labile DNA in cytologically normal nucle

A model for quantitative follow‐up studies of cervical lesions

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