Cytoplasmic argyrophilic protein(s) in cumulus-oocyte complexes: a preliminary study

Abstract

Summary ― The presence of acidic argyrophilic protein(s) in cumulus cells and oocytes was examined in bovine, ovine, porcine and murine cumulus-oocyte complexes (COCs) using a specific silverstaining method. The COCs were isolated from small (1 mm) or large (3-5 mm) antral follicles; they were fixed immediately after slaughter (first group) or after transfer to the laboratory (ca 60 min, second group) and then silver stained according to Likovsky'and Smetana