Cytoplasmic bags: Containers for discarded paraflagellar membranes in spermiogenesis of graphosome lineatum (Pentatomidae, Hemiptera, Insecta)

Abstract

The process of cytoplasmic sloughing is described in spermiogenesis of a stink bug, Graphosoma lineatum, using transmission electron microscopy of ultrathin sections. Tails of young spermatids possess a wide cytoplasmic layer lateral to the axoneme and the nenbenkern derivatives. Membranous sheets, comprised of cisternae of endoplasmic reticulum with very narrow lumina, are arranged parallel to these organelles. More advanced spermatids show only a thin cytoplasmic layer largely devoid of membranes. At this stage, large evaginations of the flagellar membrane, termed cytoplasmic bags, are found in association with the spermatid tails. The most prominent elements within these bags are concentric layers of endoplasmic reticulum of the type previously found in spermatid tails. This relationship suggests that the cells rid themselves of cytoplasmic membranes throughout spermiogenesis via inclusion into cytoplasmic bags. Upon release from the nucleate cytoplasm, the cytoplasmic bags become more and more electron-dense and degenerate. © 1995 Wiley-Liss, Inc.