Cytoplasmic Genes and Cell Heredity

A mutant of Euglena gracilis Z is described that spontaneously produces irreversibly bleached mutants at a high frequency. Bleaching only occurs during exponential growth under highly aerobic conditions. The kinetics for bleaching and growth of bleached and nonbleached cells suggest that mutation is induced by segregation of a cellular component that becomes limiting after several culture generations. The properties of spontaneous bleaching closely resemble the properties of induced organelle mutagenesis in E. gracilis and yeast. Mitochondria and chloroplast are semiautonomous organelles of eukaryote cells and propagate by duplication (6). These organelles contain deoxyribonucleic acid (DNA), ribonucleic acid (RNA), ribosomes, and other molecular components required for gene expression (18). A variety of chemical and physical treatments of Euglena gracilis induce formation of bleached mutants that do not contain chloroplasts (13). Treatments that are commonly

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Subcellular Localization of Isoleucine-Valine Biosynthetic Enzymes in Yeast

Ryan

Kohlhaw

1974

J Bacteriol

118

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By using the method of stepwise homogenization of yeast spheroplast lysates employed previously with the leucine biosynthetic enzymes, it is shown that threonine deaminase, acetohydroxy acid synthase, Mg2+-dependent isomeroreductase, and dihydroxy acid dehydratase are particulate. Density gradient centrifugation and the behavior of marker enzymes suggest that all of the above enzymes of the isoleucine-valine biosynthetic pathway are associated with the mitochondria.on July 16, 2020 by guest

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Cytoplasmic Genes and Cell Heredity

Cited by 6 publications

References 48 publications

Molecular genetics of chloroplasts and mitochondria in the unicellular green algaChlamydomonas

Molecular genetics of chloroplasts and mitochondria in the unicellular green algaChlamydomonas

An Unusual Mutant Affecting the Frequency of Organelle Mutation

Subcellular Localization of Isoleucine-Valine Biosynthetic Enzymes in Yeast

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