Cytoplasmic inheritance of oligomycin resistance in Chinese hamster ovary cells.

Abstract

Oligomycin-resistant clones were isolated from Chinese hamster ovary cells by treatment of cells with ethidium bromide, followed by mutagenesis with ethylmethane sulfonate and selection in oligomycin . One clone (Oli' 8

Genetics of the mammalian oxidative phosphorylation system: characterization of a new oligomycin-resistant Chinese hamster ovary cell line.

“…oli3 cells differ from olil cells in being cross-resistant to venturicidin. Oligomycin resistance in S. cerevisiae can also be conferred by a mutation in the nuclear DNA, although no increase in resistance of the mitochondrial ATPase has been observed (2,13,40,45 (9,51), antimycin (24), rutamycin (33), erythromycin (19), and oligomycin (7,27), and mutants with defects in mitochondrial protein synthesis (52). Chloramphenicol resistance has recently been shown to be due to a mutation in the 16S rRNA gene of the mitochondrial DNA (4,29).…”

“…When cells are exposed to an inhibitor of oxidative phosphorylation, such as oligomycin, energy must be derived exclusively from glycolysis. Because the rate of glycolysis is slower when galactose is used as a carbon source than when glucose is used, cells are more sensitive to such inhibitors in medium containing galactose (7,27), chloramphenicol (53), and antimycin (25). As shown in Fig.…”

“…It is possible that this double mutation arose as a result of the double mutagenic treatment; ethidium bromide might have caused a mutation in the mitochondrial DNA and ethyl methane sulfonate might have caused a mutation in the nuclear DNA. Interestingly, however, another oligomycin-resistant CHO cell line recovered in the same selection has only a cytoplasmic mutation (7).…”

“…(7). This mutant is specifically resistant to oligomycin and closely related ATPase inhibitors and is cytoplasmically inherited (7).…”

Nuclear Inheritance of Erythromycin Resistance in Human Cells: New Class of Mitochondrial Protein Synthesis Mutants

“…A similar approach has been used to isolate mammalian cells with cytoplasmically inherited mutations. These include mutants which are resistant to the mitochondrial protein synthesis inhibitors chloramphenicol (7,16,18,25) and erythromycin (9), mutants deficient in mitochondrial protein synthesis (30), and mutants which are resistant to inhibitors of respiration (12) and oxidative phosphorylation (4,14).…”