Cytoplasmic male sterile and doubled haploid lines with desirable combining ability enhances the concentration of important antioxidant attributes in Brassica oleracea

Doubled haploid based parental lines are most suitable in predicting heterosis using microsatellites and in development of highly heterotic F1hybrids inBrassica oleracea

“…Of the diverse species, Brassica oleracea (CC, 2n = 18) constitutes well defined group of economically and nutritionally important morphotypes, referred to as cole vegetables (kale, kohlrabi, cabbage, cauliflower, broccoli, brussels sprout) [52]. These Brassica vegetables are also termed as ‘super-food’ as they are vital source of secondary metabolites, antioxidants, vitamins and minerals [71, 16, 64, 69]. Among the cultivated B. oleracea varieties, cauliflower ( B. oleracea var.…”

“…Great efforts have been made to improve the productivity and quality of this crop, ascribed to its economic value and as an essential component of healthy diet [91]. The replacement of open pollinated varieties with F 1 hybrids have become much pronounced in cole vegetables including cauliflower due to high uniformity, better quality, tolerance to various biotic stresses and vagaries of adverse climatic conditions [17, 69]. It is well established that Brassica vegetables exhibit a wide range of heterosis and high heterosis have been reported in cauliflower also for both yield and quality traits [13, 15, 69].…”

“…The replacement of open pollinated varieties with F 1 hybrids have become much pronounced in cole vegetables including cauliflower due to high uniformity, better quality, tolerance to various biotic stresses and vagaries of adverse climatic conditions [17, 69]. It is well established that Brassica vegetables exhibit a wide range of heterosis and high heterosis have been reported in cauliflower also for both yield and quality traits [13, 15, 69]. Nature has bestowed Brassica vegetables with genetic mechanisms of sporophytic self-incompatibility (SI) and cytoplasmic male-sterility (CMS), which have efficiently triggered the hybrid breeding programme in these crops [13, 15, 17, 66, 69, 77].…”

“…It is well established that Brassica vegetables exhibit a wide range of heterosis and high heterosis have been reported in cauliflower also for both yield and quality traits [13, 15, 69]. Nature has bestowed Brassica vegetables with genetic mechanisms of sporophytic self-incompatibility (SI) and cytoplasmic male-sterility (CMS), which have efficiently triggered the hybrid breeding programme in these crops [13, 15, 17, 66, 69, 77]. However, in the current scenario of increasing temperature as a result of global warming there is frequent breakdown of self-incompatibility, as S-alleles are more prone to high temperature.…”

“…In addition, the maintenance of S-allele lines is time consuming and costly endeavor and in case of snowball cauliflower, SI system is very poor or absent [66, 68]. Under these circumstances, the genetic system of CMS provides a better alternative for heterosis breeding in cole crops [17, 69]. Heterosis or hybrid vigor, plausibly results from accumulation of parental genetic and epigenetic information, is manifested as superior performance of hybrid offspring relative to the average of their genetically diverse parents [3, 26, 45].…”