Cytoplasmic Male Sterilities and Mitochondrial Gene Mutations in Plants

Budar, Françoise; Berthomé, Richard

doi:10.1002/9780470986592.ch9

Cited by 21 publications

(14 citation statements)

References 203 publications

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“…The results presented here add to the developing understanding of the basis of pollen sterility in plants. Mitochondrial activity is required during pollen development and has been linked to male sterility, mainly through studies of CMS‐plants and their restorer lines (Hanson and Bentolila 2004; Budar and Berthome 2007; Chase 2007; Kubo and Newton 2008; Mackenzie 2011). The molecular basis for pollen sterility still remains elusive, although it seems logical that altered respiration activities would result with reduced pollen production characteristics.…”

Section: Discussionmentioning

confidence: 99%

“…CMS‐plants are also valuable agriculturally, as these provide an economical means to producie hybrid seeds. The genetic factors for male sterility are commonly identified as novel “chimeric‐ORFs” within the mitochondrial genomes, whereas nuclear genes able to restore fertility (Rfs) counteract the “sterility factors” by affecting the expression of CMS‐related genes (Hanson and Bentolila 2004; Budar and Berthome 2007; Chase 2007; Kubo and Newton 2008; Mackenzie 2011).…”

Section: Introductionmentioning

confidence: 99%

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Expression of Mitochondrial Gene Fragments within the Tapetum Induce Male Sterility by Limiting the Biogenesis of the Respiratory Machinery in Transgenic Tobacco^F

Shaya

Gaiduk

Keren

et al. 2012

JIPB

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Plant mitochondrial genomes (mtDNAs) are large and undergo frequent recombination events. A common phenotype that emerges as a consequence of altered mtDNA structure is cytoplasmic‐male sterility (CMS). The molecular basis for CMS remains unclear, but it seems logical that altered respiration activities would result in reduced pollen production. Analysis of tobacco (Nicotiana tabacum) mtDNAs indicated that CMS‐associated loci often contain fragments of known organellar genes. These may assemble with organellar complexes and thereby interfere with normal respiratory functions. Here, we analyzed whether the expression of truncated fragments of mitochondrial genes (i.e. atp4, cox1 and rps3) may induce male sterility by limiting the biogenesis of the respiratory machinery. cDNA fragments corresponding to atp4f, cox1f and rps3f were cloned in‐frame to a mitochondrial localization signal and a C‐termini HA‐tag under a tapetum‐specific promoter and introduced to tobacco plants by Agrobacterium‐mediated transformation. The constructs were then analyzed for their effect on mitochondrial activity and pollen fertility. Atp4f, Cox1f and Rps3f plants demonstrated male sterility phenotypes, which were tightly correlated with the expression of the recombinant fragments in the floral meristem. Fractionation of native organellar extracts showed that the recombinant ATP4f‐HA, COX1f‐HA and RPS3f‐HA proteins are found in large membrane‐associated particles. Analysis of the respiratory activities and protein profiles indicated that organellar complex I was altered in Atp4f, Cox1f and Rps3f plants.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Expression of Mitochondrial Gene Fragments within the Tapetum Induce Male Sterility by Limiting the Biogenesis of the Respiratory Machinery in Transgenic Tobacco^F

Shaya

Gaiduk

Keren

et al. 2012

JIPB

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“…CMS, which shows a phenotype of pollen abortion in plants that are otherwise developmentally normal, is one representative of this class of mutations. CMS has been reported in more than 140 plant species [36], and the number of identified genes associated with the phenotype has reached 23, of which 21 involve unique ORFs [73]. An example is the maize urf13-T gene, which contains two duplicated segments of rrn26, forming an ORF that encodes 115 amino acid residues [74].…”

Section: Why Do Most Of the Unique Orfs Not Matter In Angiosperm Mitomentioning

confidence: 99%

Cost of Having the Largest Mitochondrial Genome: Evolutionary Mechanism of Plant Mitochondrial Genome

Kitazaki

Kubo

2010

Journal of Botany

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The angiosperm mitochondrial genome is the largest and least gene-dense among the eukaryotes, because its intergenic regions are expanded. There seems to be no functional constraint on the size of the intergenic regions; angiosperms maintain the large mitochondrial genome size by a currently unknown mechanism. After a brief description of the angiosperm mitochondrial genome, this review focuses on our current knowledge of the mechanisms that control the maintenance and alteration of the genome. In both processes, the control of homologous recombination is crucial in terms of site and frequency. The copy numbers of various types of mitochondrial DNA molecules may also be controlled, especially during transmission of the mitochondrial genome from one generation to the next. An important characteristic of angiosperm mitochondria is that they contain polypeptides that are translated from open reading frames created as byproducts of genome alteration and that are generally nonfunctional. Such polypeptides have potential to evolve into functional ones responsible for mitochondrially encoded traits such as cytoplasmic male sterility or may be remnants of the former functional polypeptides.

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“…, 1998). Nevertheless, the cost of restoration as a factor maintaining gynodioecy is appealing in the light of recent biochemical studies showing the existence of negative pleiotropic effects associated with MS in Arabidopsis and in many crop species (see Budar & Berthomé, 2007; Ivanov & Dymshits, 2007).…”

Section: Introductionmentioning

confidence: 99%

Evidence of restoration cost in the annual gynodioecious Phacelia dubia

Castillo

Trujillo

2009

J of Evolutionary Biology

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A negative pleiotropic effect on fitness of nuclear sex‐determining genes (cost of restoration) could explain nuclear–cytoplasmic gynodioecy but rarely has been demonstrated empirically. In a gynodioecious Phacelia dubia population, maternal lineages produce only hermaphroditic progenies irrespective of the pollen parent (N) or can segregate females (S). Natural progenies of N maternal plants had lower seed viability than that of S. Full‐sib progenies of unrelated hermaphrodites from all possible matings between N and S lineages had similar pollen filling but differed in sporophyte performance, mainly at seed germination stage. A discrete multivariate analysis reveals that the performance of N♀ × S♂ progeny at early stages of development was significantly lower than that of the other three types of mating in agreement with the silent‐cost‐of‐restoration hypothesis, affecting the sporophyte. The restoration cost and male sterility appear to be dominant and consequence of nuclear–cytoplasmic incompatibilities that may maintain nuclear–cytoplasmic polymorphism by frequency‐dependent selection.

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Cytoplasmic Male Sterilities and Mitochondrial Gene Mutations in Plants

Cited by 21 publications

References 203 publications

Expression of Mitochondrial Gene Fragments within the Tapetum Induce Male Sterility by Limiting the Biogenesis of the Respiratory Machinery in Transgenic Tobacco^F

Expression of Mitochondrial Gene Fragments within the Tapetum Induce Male Sterility by Limiting the Biogenesis of the Respiratory Machinery in Transgenic Tobacco^F

Cost of Having the Largest Mitochondrial Genome: Evolutionary Mechanism of Plant Mitochondrial Genome

Evidence of restoration cost in the annual gynodioecious Phacelia dubia

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Cytoplasmic Male Sterilities and Mitochondrial Gene Mutations in Plants

Cited by 21 publications

References 203 publications

Expression of Mitochondrial Gene Fragments within the Tapetum Induce Male Sterility by Limiting the Biogenesis of the Respiratory Machinery in Transgenic TobaccoF

Expression of Mitochondrial Gene Fragments within the Tapetum Induce Male Sterility by Limiting the Biogenesis of the Respiratory Machinery in Transgenic TobaccoF

Cost of Having the Largest Mitochondrial Genome: Evolutionary Mechanism of Plant Mitochondrial Genome

Evidence of restoration cost in the annual gynodioecious Phacelia dubia

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Expression of Mitochondrial Gene Fragments within the Tapetum Induce Male Sterility by Limiting the Biogenesis of the Respiratory Machinery in Transgenic Tobacco^F

Expression of Mitochondrial Gene Fragments within the Tapetum Induce Male Sterility by Limiting the Biogenesis of the Respiratory Machinery in Transgenic Tobacco^F