Cytoplasmic microtubule assembly is altered in cytoplasts

Roy, Sujata Guha; Bhisey, A.N.

doi:10.1016/s0309-1651(06)80128-1

Cited by 1 publication

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“…1984, Gelfand etal. 1985, Bershadsky and Vasiliev 1988, Guha Roy and Bhisey 1992, and references therein), but investigation of two types of plant cytoptasts suggests that the plant cell cytoskeleton has an intrinsic capacity for self-organization (Haemanthus endosperm: Bajer andMol6-Bajer 1982, 1986; pollen cytoplasts: Rutten andDerksen 1990, 1992). To better understand the "autonomous" properties of the plant eytoskeleton we focused on the ability of cytoplasts of the filamentous green alga Mougeotia to participate in the rapid, highly ordered process of protoplast regeneration that is characteristic of this species.…”

Section: Introductionmentioning

confidence: 99%

Capacity for microtubule reorganization and cell wall synthesis in cytoplasts of the green algaMougeotia

1994

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Sunnnary. A small proportion of nucleate subprotoplasts (karyoplasts) and enucleate subprotoptasts (cytoplasts) are formed during the preparation ofprotoplasts from the filamentous green alga Mougeotia. Regeneration of Mougeotia protoplasts is an orderly process known to involve reorganisation of cortical microtubules into polar arrays centered upon two opposing foci, synthesis of new cell walls and elongation to reform cylindrical cells. The ability of cytoplasts to carry out microtubule reorganisation and cell wall synthesis was investigated by combining Hoechst staining, to distinguish cytoplasts from karyoplasts and protoplasts, with immunofluorescent staining of microtubules and Calcofiuor or Tinopal staining of cell walls. Cytoplasts survived at least 20 h in culture, but did not elongate. However, cytoplasts did participate in the first steps of protoplast regeneration. The majority of cytoplasts synthesized some cell wall material, while a small proportion was able to form ordered arrays of cortical microtubules indistinguishable from those in regenerating nucleate protoplasts. These results demonstrate the ability of plant microtubules to form new, orderly arrays in the absence of a nucleus, and suggest that the reestablishment of axiality in the protoplasts does not require a nucleus or nuclear DNA transcription.

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