Cytoplasmic transfer of chloramphenicol resistance in human tissue culture cells.

Abstract

The cytoplasmic inheritance of human chloramphenicol (CAP) resistance has been demonstrated by removing the nuclei of cells of the CAP-resistant HeLa strain 296-1 (enucleation) and fusing them to a CAP-sensitive HeLa strain lacking nuclear thymidine kinase. Plating the fusion products in bromodeoxyuridine and CAP resulted in the growth of about 150 colonies/106 parent cells plated. Permanent cell lines (cybrids) grown from such fusions have been designated HEB.

Genetics of the mammalian oxidative phosphorylation system: characterization of a new oligomycin-resistant Chinese hamster ovary cell line.

“…oli3 cells differ from olil cells in being cross-resistant to venturicidin. Oligomycin resistance in S. cerevisiae can also be conferred by a mutation in the nuclear DNA, although no increase in resistance of the mitochondrial ATPase has been observed (2,13,40,45 (9,51), antimycin (24), rutamycin (33), erythromycin (19), and oligomycin (7,27), and mutants with defects in mitochondrial protein synthesis (52). Chloramphenicol resistance has recently been shown to be due to a mutation in the 16S rRNA gene of the mitochondrial DNA (4,29).…”

The Potential Application of Mitochondrial Medicine in Toxicologic Poisoning

“…Cybrids have been used as early as the 1970s to study cytoplasmic inheritance of antibiotic resistance in various tissue culture cells [12,13]. Cybrids have also been used to study disease states that involve known mtDNA mutations.…”

Does Mitochondrial DNA Play a Role in Parkinson's Disease? A Review of Cybrid and Other Supportive Evidence

“…In one early cybrid study, enucleated cytoplasts from a chloramphenicol-resistant HeLa cell strain were mixed with the intact cells of a chloramphenicol-sensitive HeLa strain. The resulting cybrid cell line showed stable chloramphenicol resistance and illustrated in cultured cells choramphenicol sensitivity versus tolerance is determined by mtDNA (157).…”