Cytopreparatory Techniques

Gill, Gary W.; Crabtree, William N.; Kelly, Deidra

doi:10.1007/978-3-319-22864-8_10

Cited by 2 publications

(2 citation statements)

References 9 publications

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“…Among the 3 dyes of Pap staining, light green is more soluble in water than eosin or orange G, and its discoloration tends to be rapid. In previous studies, methods such as the addition of 33% glycerine [1] or a surfactant (Emulgen 810) [2] to the stain solution were used, but stainability was unstable among discolored specimens. Therefore, the present method is very useful.…”

Section: Discussionmentioning

confidence: 99%

“…However, if a technique is developed enabling the restaining of precious and/or rare specimens or those for education to restore their former state, it may be extremely useful for the reuse of specimens. Techniques such as decolorization with 1% hydrochloric acid followed by restaining of Papanicolaou (Pap) specimens, the addition of 33% glycerine [1] or a surfactant to the Pap stain [2], or treatment with phosphate buffer (reported only in domestic society meetings) have been used. However, the reproducibility of restoration to the state before discoloration was low, and there have been only a few published papers.…”

Section: Introductionmentioning

confidence: 99%

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New Method to Restore Fading Papanicolaou Specimen Using an Optical Clearing Agentx

Washiya¹

2014

J Cytol Histol

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Cytological specimens are used only once for staining, and cannot be reused after preservation in paraffin blocks. If a technique is developed allowing restaining of precious/rare specimens or those for education, which have been discolored due to long-term preservation, to restore their former states, it will be extremely useful for the reuse of specimens. We performed this study to determine whether discolored specimens can be restored to their former condition by applying an optical clearing agent. For restaining, 131 Papanicolaou (Pap) specimens prepared from 13 to more than 30 years ago were used. Using an optical clearing agent (SCALEVIEW-A2), Pap restaining, endurance tests of the optical clearing agent by repeated use, and immunostaining were performed. After treatment with the optical clearing agent for5-10 minutes, discolored cytological specimens could be stably restained just as the initial Pap specimens. As a result of the immunostaining of specimens treated using this method, both the nucleus (Ki-67) and cell membrane (MOC31) were positively stained. The optical clearing agent did not deteriorate when used repeatedly ten times. Discolored precious cytological specimens could be restored using a optical clearing agent. Specimens processed using this method could also be immunostained, and so this method is extremely useful.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

New Method to Restore Fading Papanicolaou Specimen Using an Optical Clearing Agentx

Washiya¹

2014

J Cytol Histol

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Fine needle aspiration cytology of cervical lymph nodes: Comparison of liquid based cytology (SurePath) and conventional preparation

Budhwar

Kataria

Kumar

et al. 2020

Diagnostic Cytopathology

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Background Fine needle aspiration cytology (FNAC) is the first diagnostic step in patient with cervical lymphadenopathy because of its simplicity, safety and early availability of the results. Liquid‐based cytology (LBC) is an alternative processing method which is used for both gynecological and nongynecological samples. Literature reviewed show few studies comparing LBC with conventional preparation (CP). Aim The present study was undertaken to evaluate the efficacy of LBC and comparison of LBC and CP in cervical lymphadenopathy. Materials and Methods In this prospective study, a total of 75 cases of FNAC with cervical lymphadenopathy were included. The first pass was used for CP followed by LBC with the use of SurePath (SP) technique. Both the smears were compared for cellularity, background containing blood, cell debris, lymphoglandular bodies, stromal fragments, cytoarchitectural pattern, etc., by semiquantitative scoring system. Results There was no statistical difference in the cellularity, cell architecture, and monolayer cells (P > .05). On the basis of background containing blood, cell debris, lympho‐glandular bodies, stromal fragments (P < .001), nuclear, and cytoplasmic details (P < .05), LBC was found to be superior to CP. Conclusion LBC is a relatively simple technique and superior to CP in respect of better nuclear and cytoplasmic details with loss of background blood and debris. It has a diagnostic accuracy equivalent to that of CP. However, use of both LBC and CP can result in better diagnostic accuracy.

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Cytopreparatory Techniques

Cited by 2 publications

References 9 publications

New Method to Restore Fading Papanicolaou Specimen Using an Optical Clearing Agentx

New Method to Restore Fading Papanicolaou Specimen Using an Optical Clearing Agentx

Fine needle aspiration cytology of cervical lymph nodes: Comparison of liquid based cytology (SurePath) and conventional preparation

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