Cytoprotective and Anti-inflammatory Effects of 6-Shogaol on Human Dermal Fibroblasts

Abstract

Purpose: 6-Shogaol is one of the spicy flavor ingredients of ginger and hydrolysis product of gingerols. The purpose of this study is to investigate the potential of 6-shogaol as a natural cosmetic raw material by examining cell protection and inflammation inhibitory activity in human dermal fibroblasts (HDFs). Methods: To verify the cytoprotective effects of 6-shogaol, cell viability was measured by watersoluble tetrazolium salt (WST-1) assay. To verify the anti-inflammatory effects of 6-shogaol, we performed nuclear factor kappa-light-chain-enhancer of activated B cells (NFΚB) promoter luciferase assay to check the expression of NFΚB. Additionally, the expression of inflammation related gene such as cyclooxygenase 2 (COX2), receptor for advanced glycation end product (RAGE), tumor necrosis factor alpha (TNFΑ), interleukin 6 (IL6), and interleukin 8 (IL8) mRNA was measured by quantitative real-time polymerase chain reaction (qRT-PCR). Results: 6-Shogaol showed no toxicity in HDFs at concentrations of 5, 10, 20, and 40 μM, respectively and the cell viability was increased in a dose dependent manner. qRT-PCR analysis showed 6-shogaol treatment downregulated the expression of NFΚB, COX2, RAGE, TNFΑ, IL6, and IL8 in a dose dependent manner, resulting that 6-shogaol leads to protective activities against inflammatory responses in HDFs. Conclusion: As mentioned above, 6-shogaol restored cell viability and decreased the expression of inflammatory factors in a dose dependent manner. Consequently, these results suggest the possibility of 6-shogaol as cosmetic material preventing skin aging, through identified functions on cytoprotection and anti-inflammation.

Anti-apoptotic, antioxidant and anti-aging effects of 6-shogaol on human dermal fibroblasts

“…6-Shogaol was purchased in a powder form (> 90% purity) from Sigma-Aldrich Inc. (St. Louis, MO, USA) and dissolved in dimethyl sulfoxide (Sigma-Aldrich). UVA irradiation was performed using a UV-A lamp (UVP, USA) at a dose of 10 J/cm 2 , based on a previous study (Lee et al 2017).…”

The Effect of the Mixed Extract of Kalopanax pictus Nakai and Achyranthes japonica Nakai on the Improvement of Degenerative Osteoarthritis through Inflammation Inhibition in the Monosodium Iodoacetate-Induced Mouse Model

“…Another herb, Boswellia serrata, has been reported to exhibit anti-inflammatory effects and may have a beneficial impact on osteoarthritis [ 37 , 38 ]. Furthermore, Zingiber officinale (ginger) has shown anti-inflammatory activity and may contribute to the management of osteoarthritis symptoms [ 39 ]. These studies highlight the potential of various herbs in modulating inflammation and alleviating osteoarthritis symptoms.…”

Effect of Applying Various Time of Retinol Pretreatment on Improve Skin after Herbal Peeling

“…그러나 약초필링도 다른 필링과 같이 필링 후 나타나는 피부 감 작성에서 완전히 배제될 수 없는데, 필링은 피부를 개선시키는 반면 홍반, 수분 부족, 피부 자극의 부작용을 동반한다 (Deprez, 2007;Farber, 1998 (Kim et al, 2009 (Kim et al, 2012;Lee et al, 2017;Park et al, 2003). Kim et al (1997) (Duke & Grevelink, 1998 (Heinrich et al, 2003).…”