Cytoprotective and genoprotective effects of taxifolin against oxidative damage in HTR-8/SVneo human trophoblast cells

Abstract

An increase of reactive oxygen species in the placenta and oxidative disbalance has been recognized as a significant factor contributing to pregnancy complications. Dietary intake of food rich in antioxidants during pregnancy could exert a protective role in the prevention of adverse outcomes such as preeclampsia, miscarriage, and others. Flavonoid taxifolin has shown numerous health-promoting effects in a large number of studies conducted on animals, as well as various human cell types in vitro. However, its effects on human placental cells—trophoblasts—have yet to be determined. Therefore, cytoprotective and genoprotective effects of taxifolin on trophoblast cell line HTR-8/SVneo under induced oxidative stress were explored in this study. Cytotoxicity of a range of taxifolin concentrations (1–150 µM) was evaluated using the MTT and crystal violet assays. A model of oxidative stress was achieved by exposing HTR-8/SVneo cells to H2O2. To determine cytoprotective and antigenotoxic effects, the cells were pre-incubated with three concentrations of taxifolin (10, 50, and 100 µM) and then exposed to H2O2. Taxifolin in concentrations of 1, 5, 10, 25, 50, and 100 µM showed no cytotoxic effects on HTR-8/SVneo cells, but 150 µM of taxifolin caused a significant decrease in adherent cell number, as detected by crystal violet assay. Pretreatment with the chosen concentrations of taxifolin showed a significant cytoprotective effect on H2O2-induced cytotoxicity, as determined by the MTT assay. Furthermore, taxifolin showed a significant reduction in H2O2-induced DNA damage, measured by comet assay. This study showed protective effects of taxifolin on human trophoblast cells exposed to oxidative damage. Further studies are needed to explore the underlying mechanisms.

Antioxidant Supplementation Alleviates Mercury-Induced Cytotoxicity and Restores the Implantation-Related Functions of Primary Human Endometrial Cells

“…These results support the decreased viability, aberrant ROS production, and enhanced cell death reported herein and in previously published studies using the HTR8/SV-neo trophoblast cell line [31,45]. The antioxidant-mediated restoration of JEG-3 trophoblastic spheroid expansion in our co-culture model also corroborates the beneficial effects of antioxidants reported in trophoblast cell cultures [46,47]. Taken together, this evidence supports the notion that Hg-induced endometrial cell damage can be substantially reversed with antioxidant supplementation.…”

Oleuropein Stimulates Migration of Human Trophoblast Cells and Expression of Invasion-Associated Markers

“…Crystal violet assay was used to assess cell viability after the treatment with OLE in a range of concentrations (1–200 µM), as described previously [ 68 ]. The cells were harvested from flasks after reaching 70% confluence, by using 0.25% trypsin-EDTA solution (Capricorn Scientific, Ebsdorfergrund, Germany) and counted.…”