Cytoprotective effect and purification of novel antioxidant peptides from hazelnut (C. heterophylla Fisch) protein hydrolysates

Liu, Chunlei; Ren, Dayong; Li, Jingjing; Li, Fang; Wang, Ji; Liu, Jingsheng; Min, Weihong

doi:10.1016/j.jff.2017.12.003

Cited by 90 publications

(68 citation statements)

References 36 publications

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“…As a consequence, such high‐quality protein resources are wasted. In our previous research, hazelnut protein isolates were prepared from defatted hazelnut dregs by the alkali‐soluble acid precipitation method (Liu et al, ; Ren et al, ). Crude protein hydrolysates obtained by the hydrolysis of isolated hazelnut protein by alcalase have been proved to have considerable immunomodulatory and antioxidant activities (Liu et al, ; Ren et al, ).…”

Section: Introductionmentioning

confidence: 99%

“…In our previous research, hazelnut protein isolates were prepared from defatted hazelnut dregs by the alkali‐soluble acid precipitation method (Liu et al, ; Ren et al, ). Crude protein hydrolysates obtained by the hydrolysis of isolated hazelnut protein by alcalase have been proved to have considerable immunomodulatory and antioxidant activities (Liu et al, ; Ren et al, ). Recently, the peptides (Asp‐Trp‐Asp‐Pro‐Lys [DWDPK], Ala‐Asp‐Gly‐Phe [ADGF], Ser‐Gly‐Ala‐Phe [SGAF], Glu‐Thr‐Thr‐Leu [ETTL], and Ala‐Gly‐Gly‐Phe [AGGF]) were purified from hazelnut protein hydrolysates and identified (Liu et al, ), and Glu‐Trp (EW) were purified from a different batch of hazelnut protein hydrolysates prepared in our lab and was identified.…”

Section: Introductionmentioning

confidence: 99%

“…Crude protein hydrolysates obtained by the hydrolysis of isolated hazelnut protein by alcalase have been proved to have considerable immunomodulatory and antioxidant activities (Liu et al, ; Ren et al, ). Recently, the peptides (Asp‐Trp‐Asp‐Pro‐Lys [DWDPK], Ala‐Asp‐Gly‐Phe [ADGF], Ser‐Gly‐Ala‐Phe [SGAF], Glu‐Thr‐Thr‐Leu [ETTL], and Ala‐Gly‐Gly‐Phe [AGGF]) were purified from hazelnut protein hydrolysates and identified (Liu et al, ), and Glu‐Trp (EW) were purified from a different batch of hazelnut protein hydrolysates prepared in our lab and was identified. The six peptides are novel peptides with very low similarity in sequence to other antioxidant peptides.…”

Section: Introductionmentioning

confidence: 99%

“…In our previous research, hazelnut protein isolates were prepared from defatted hazelnut dregs by the alkali-soluble acid precipitation method (Liu et al, 2018;Ren et al, 2016). Crude protein hydrolysates obtained by the hydrolysis of isolated hazelnut protein by alcalase have been proved to have considerable immunomodulatory and antioxidant activities (Liu et al, 2018;Ren et al, 2016). Recently, the peptides (Asp-Trp-Asp-Pro-Lys [DWDPK], Ala-Asp-Gly-Phe [ADGF], Ser-Gly-Ala-Phe [SGAF], Glu-Thr-Thr-Leu [ETTL], and Ala-Gly-Gly-Phe…”

Section: Introductionmentioning

confidence: 99%

“…[AGGF]) were purified from hazelnut protein hydrolysates and identified (Liu et al, 2018), and Glu-Trp (EW) were purified from a different batch of hazelnut protein hydrolysates prepared in our lab and was identified. The six peptides are novel peptides with very low similarity in sequence to other antioxidant peptides.…”

Section: Introductionmentioning

confidence: 99%

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Protective role of hazelnut peptides on oxidative stress injury in human umbilical vein endothelial cells

Ren

Wang

et al. 2018

J Food Biochem

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The crude protein hydrolysates of wild hazel have good immunoregulation and antioxidation effects. However, the components responsible for their antioxidation effect remain unknown. In this study, six antioxidative peptides (EW, DWDPK, ADGF, SGAF, ETTL, and AGGF) were tested for their protective effects on oxidative stress injury in human umbilical vein endothelial cells (HUVECs). The results demonstrated that the six peptides are nontoxic and have a protective effect on oxidative stress injury induced by Ang II. Three peptides (EW, ADGF, and DWDPK) inhibited the morphological changes, downregulated the content of lactate dehydrogenase and malondialdehyde, upregulated the activity of antioxidant enzymes catalase, total superoxide dismutase and glutathione peroxidase, and scavenged reactive oxygen species (ROS) in HUVECs. Quantitative reverse transcriptive polymerase chain reaction and western blot assays indicated that these three peptides regulated NADPH oxidase activity and ROS production by reducing NOX4 and p22phox levels. Overall, they have a significant protective effect against oxidative stress injury and have potential application in developing new functional foods. Practical applications Corylus heterophylla Fisch is a good quality wild hazel distributed in Northeast China. Wild hazelnut of the species C. heterophylla Fisch was selected as experimental object and has high nutritive values and have abundant proteins (20%–30%), fats (40%–50%), carbohydrates (13%–24%), dietary fibers (8.2%–9.6%), vitamins, and micronutrients. Our results indicate that hazelnut peptides (EW, ADGF, and DWDPK) can ensure normal growth of cells by protecting important antioxidant enzyme systems, by enhancing antioxidant defense, by directly affecting nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activity, and by reducing intracellular reactive oxygen species (ROS) production in HUVECs, indicating that the three antioxidative peptides have a protective effect against Ang II‐induced oxidative stress injury. Therefore, the antioxidative peptides from C. heterophylla Fisch may be a promising candidate for functional food ingredients and/or pharmaceuticals.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Protective role of hazelnut peptides on oxidative stress injury in human umbilical vein endothelial cells

Ren

Wang

et al. 2018

J Food Biochem

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Plant‐derived peptides for the improvement of Alzheimer's disease: Production, functions, and mechanisms

Lin

Liu

et al. 2023

Food Frontiers

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Alzheimer's disease (AD) is a progressive neurodegenerative disease with an insidious onset that seriously affects human health. Plant-derived peptides have been found to hinder the development of AD pathologies, which is an excellent candidate for preventing AD. However, due to the excessively complicated pathogenesis of AD and the fact that most studies on the activity of plant-derived peptides are single and not deep enough, which restricted the development and application of plant-derived AD-prevention peptides (PADPs). This review summarized the currently available means of obtaining PADPs, in vitro and in vivo AD-prevention activity validation protocols, molecular pathways of PADPs, the structure-activity relationship between plant-derived peptides and AD-prevention activity, and some perspectives of current advanced technologies. This paper will help to develop the foundation for the production and exploitation of PADPs.

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Protective effect of antioxidant peptides from bass (Lateolabrax japonicus) on oxidative stress injury in Caco‐2 cells

Chen

et al. 2023

Food Frontiers

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Excessive reactive oxygen species (ROS) can induce oxidative damage to cell membranes and biomolecules, leading to various chronic diseases. Therefore, the exploration of effective natural antioxidants that can replace traditional synthetic antioxidants to reduce excess free radicals without side effects is important. Herein, the antioxidant peptide (Lateolabrax japonicus protein hydrolysates; LPH) was obtained from bass (L. japonicus) by an optimal enzymatic hydrolysis process. The protective effect of LPH on the Caco-2 cell oxidative stress model and the potential antioxidant signaling pathways were explored. The results showed that LPH was rich in hydrophobic amino acids, and the IC 50 for 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity was 0.9667 mg/mL. Furthermore, the LPH treatment reduced cellular ROS levels and lipid peroxidation while increasing antioxidant enzyme activity in a dosedependent manner (p < .05). LPH also upregulated the expression of mRNAs related to antioxidant enzymes in the Nrf2 signaling pathway (p < .05), demonstrating the efficacy of LPH on the Nrf2-ARE signaling pathway. These results suggested that LPH could be used as a potential food-derived antioxidant in the food and healthcare industries.

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Cytoprotective effect and purification of novel antioxidant peptides from hazelnut (C. heterophylla Fisch) protein hydrolysates

Cited by 90 publications

References 36 publications

Protective role of hazelnut peptides on oxidative stress injury in human umbilical vein endothelial cells

Protective role of hazelnut peptides on oxidative stress injury in human umbilical vein endothelial cells

Plant‐derived peptides for the improvement of Alzheimer's disease: Production, functions, and mechanisms

Protective effect of antioxidant peptides from bass (Lateolabrax japonicus) on oxidative stress injury in Caco‐2 cells

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