Cytoskeletal and nucleoskeletal interacting protein networks play critical roles in cellular function and dysfunction

Lambert, Muriel W.

doi:10.1177/1535370219884875

Cited by 8 publications

(7 citation statements)

References 40 publications

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“…39 By using a fluorescence resonance energy transfer system, Arsenovic and colleagues 40 have demonstrated that inhibition and activation of MLC with ML-7 or Y-27632 and calyculin A, respectively, regulates the tension of LINC, which in turn has been suggested to regulate DNA double strand break repair. 41 In this study, we also observed that CD8a T cell influx in tumors was increased by FLASH IR (Fig. 3D).…”

Section: Discussionsupporting

confidence: 73%

Effects of Ultra-high doserate FLASH Irradiation on the Tumor Microenvironment in Lewis Lung Carcinoma: Role of Myosin Light Chain

Kim

Gwak

Hong

et al. 2021

International Journal of Radiation Oncology*Biology*Physics

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Section: Discussionsupporting

confidence: 73%

Effects of Ultra-high doserate FLASH Irradiation on the Tumor Microenvironment in Lewis Lung Carcinoma: Role of Myosin Light Chain

Kim

Gwak

Hong

et al. 2021

International Journal of Radiation Oncology*Biology*Physics

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“…Two major protein networks within the cell are the cytoskeleton locating in the cytoplasm and the nucleus where the nucleoskeleton exists, and the network of proteins interacting with the cytoskeleton and nucleoskeleton plays a crucial role not only in cellular function and dysfunction, 70 but also in mechanotransduction and signaling pathways between the cytoplasm and the nucleus as well as in the regulation of transcription and gene expression. Fletcher et al 28 .…”

Section: Discussionmentioning

confidence: 99%

“…In the environment of HG-induced CCECs and CCSMCs, the osmotic pressure of cells becomes high, and the expression of SPTA1 gene is suppressed. Our transcriptome sequencing results showed that SPTA1 was enriched in the apoptosis signaling pathway, and when the expression of SPTA1 was suppressed, CCECs and CCSMCs may show cell shrinkage, cell membrane fragmentation, and SPTA1 protein cleaved by caspase-1, which may lead to the disruption of intracellular membrane structure, causing apoptosis and pyroptosis of cells, resulting in damage to CCECs and CCSMCs, and thus contributing to a decrease in erectile function.Two major protein networks within the cell are the cytoskeleton locating in the cytoplasm and the nucleus where the nucleoskeleton exists, and the network of proteins interacting with the cytoskeleton and nucleoskeleton plays a crucial role not only in cellular function and dysfunction,70 but also in mechanotransduction and signaling Expression of proteins related to the activation of Hippo signaling pathway by interfering with SPTA1 gene to induce pyroptosis and phenotypic changes in corpus cavernosum smooth muscle cells (CCSMCs). p < 0.05 was considered statistically significant, *p < 0.05, **p < 0.01, ***p < 0.001 pathways between the cytoplasm and the nucleus as well as in the regulation of transcription and gene expression.…”

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confidence: 99%

Cytoskeletal protein SPTA1 mediating the decrease in erectile function induced by high‐fat diet via Hippo signaling pathway

et al. 2022

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Background The mechanism of high‐fat diet (HFD)‐induced decrease in erectile function has not been elucidated, and in previous studies, spectrin alpha, erythrocytic 1 (SPTA1) is a cytoskeletal protein that regulates cellular function, which belongs to a family of proteins that can affect cell and tissue growth and development by regulating YAP, an effector on the Hippo signaling pathway, but its particular role has not been elucidated. Objective To explore the role of SPTA1 in the abnormality of erectile function induced by HFD. Methods We analyzed the penile tissues of mice on normal diet and HFD by transcriptomics and screened for differentially expressed genes, further identified closely related target genes in rat penile tissues, and verified target gene expression in in vitro construction of high‐glucose (HG)‐treated corpus cavernosum endothelial cells (CCECs) and corpus cavernosum smooth muscle cells (CCSMCs) models. The distribution of target genes in various cell populations in penile tissues was retrieved by single‐cell sequencing Male Health Atlas database. Moreover, interfering with target genes was further applied to explore the mechanisms involved in erectile function decline. Results Transcriptomic analysis screened out down‐regulated differential gene SPTA1; Western blot and immunohistochemistry results showed that SPTA1 expression significantly decreased in the penile tissues of Sprague–Dawley (SD) rats in the HFD group. Immunofluorescence staining showed a positive expression of CD31 and VWF in CCECs and a positive expression of α‐SMA in CCSMCs. The expression level of SPTA1 protein significantly decreased in the HG group of CCECs and CCSMCs. The expression of SPTA1 mRNA significantly decreased in CCSMCs while significantly increased in CCECs. SPTA1 may have various expression patterns and biological functions in different cell populations. Real‐time quantitative PCR results showed that the siSPTA1 transfected in CCSMCs had a significant interference effect compared with the control siNC. Transfection of siSPTA1 into CCSMCs resulted in the significant down‐regulation of mRNA and protein expression of eNOS, and significant up‐regulation of YAP, Caspase‐1, GSDMD, GSDMD‐N IL‐18, and IL‐1β protein expression levels. The expression level of CCSMCs contractile‐type protein α‐SMA was significantly down‐regulated. Conclusions The down‐regulation of SPTA1 in SD rats fed with HFD may induce cell pyroptosis and lead to the decrease of erectile function by activating the Hippo pathway; these findings may provide new therapeutic targets for improving erectile function.

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“…[4] Evidence indicates that cell damage and apoptosis are closely related to the disaggregation and rearrangement of the cytoskeleton mediated by inflammation. [5] The RhoA/Rock signaling pathway, as an important signaling pathway regulating cytoskeletal remodeling, is recognized as a crucial regulator of tissue response to injury. [6] The abnormal activation of RhoA/Rock can regulate downstream Nuclear factor-kappaB (NF-kB) signaling, leading to the progression of inflammation.…”

Section: Introductionmentioning

confidence: 99%

“…When cells undergo apoptosis, the change in cytoskeleton F‐actin is obvious, such that it is used as a sensitive indicator of apoptosis [4] . Evidence indicates that cell damage and apoptosis are closely related to the disaggregation and rearrangement of the cytoskeleton mediated by inflammation [5] . The RhoA/Rock signaling pathway, as an important signaling pathway regulating cytoskeletal remodeling, is recognized as a crucial regulator of tissue response to injury [6] .…”

Section: Introductionmentioning

confidence: 99%

Unveiling the Therapeutic Potential of Herba Epimedii: Enhancing Bone Healing Through Cytoskeletal Regulation of RhoA/Rock1 Pathway

Huang,

Lei,

Xiong

et al. 2024

Chemistry & Biodiversity

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Herba Epimedii is widely used to promote bone healing, and their active ingredients are total flavonoids of Epimedium (TFE). Ras homolog gene family member A / Rho‐associated protein kinase (RhoA/Rock), an important pathway regulating the cytoskeleton, has been proven to affect bone formation. However, whether TFE promotes bone healing via this pathway remains unclear. In this study, the therapeutic effects of TFE were estimated using micro‐computed tomography and hematoxylin and eosin staining of pathological sections. F‐actin in osteoblasts was stained to investigate the protective effects of TFE on the cytoskeleton. Its regulatory effects on the RhoA/Rock1 pathway were explored using RT‐qPCR and Western blot analysis. Besides, flow cytometry, alkaline phosphatase and nodule calcification staining were performed to evaluate the effects on osteogenesis. The bone healing in rats was improved, the cytoskeletal damage in osteoblasts was reduced, the RhoA/Rock1 pathway was downregulated, and osteogenesis was enhanced after TFE treatment. Thus, TFE can promote bone formation at least partially by regulating the expression of key genes and proteins in the cytoskeleton. The findings of this study provided evidence for clinical applications and would contribute to a better understanding of Epimedium’s mechanisms in treating bone defects.

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Cytoskeletal and nucleoskeletal interacting protein networks play critical roles in cellular function and dysfunction

Cited by 8 publications

References 40 publications

Effects of Ultra-high doserate FLASH Irradiation on the Tumor Microenvironment in Lewis Lung Carcinoma: Role of Myosin Light Chain

Effects of Ultra-high doserate FLASH Irradiation on the Tumor Microenvironment in Lewis Lung Carcinoma: Role of Myosin Light Chain

Cytoskeletal protein SPTA1 mediating the decrease in erectile function induced by high‐fat diet via Hippo signaling pathway

Unveiling the Therapeutic Potential of Herba Epimedii: Enhancing Bone Healing Through Cytoskeletal Regulation of RhoA/Rock1 Pathway

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