Cytoskeletal influences on nuclear shape in granulocytic HL-60 cells

Olins, Ada L.

doi:10.1186/1471-2121-5-30

Cited by 86 publications

(39 citation statements)

References 44 publications

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“…In accordance with a previous study (Olins and Olins 2004), four nuclear morphologies were identified: ovoid ( Fig. 2A), indented (Fig.…”

Section: Resultssupporting

confidence: 84%

“…As a model of granulocytic differentiation, we treated HL-60 cells with ATRA (1 µM) for 5 d (Mayer et al 1996). Differentiation was confirmed by observing the expected changes in nuclear morphology (Olins and Olins 2004), an increase in PMA-stimulated superoxide anion production (Mayer et al 1995), and the expected changes in the expression of the cell surface antigens CD11b, CD13, and CD15 (Brackman et al 1995;Trayner et al 1998). Quantitative real-time RT-PCR was used to analyze mRNA expression of the ECS E3 ubiquitin ligase components, ASB-2, Cul5, ROC2, and elongin B and C, and Western Blot analysis was used to examine protein expression of Cul5 in ATRA-differentiated HL-60 cells.…”

Section: Discussionmentioning

confidence: 60%

“…Finally, the slides were rinsed briefly in deionized water and allowed to air dry at room temperature. Slides were visualized using light microscopy and the nuclear morphology was classified as ovoid, indented, lobulated, or multilobed, as previously described by Olins and Olins (2004), from a total of 1,877 vehiclecontrol cells and 1,281 ATRA-treated cells.…”

Section: Methodsmentioning

confidence: 99%

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Granulocytic differentiation of HL-60 promyelocytic leukemia cells is associated with increased expression of Cul5

Baxter

Carlson

Mayer

et al. 2009

In Vitro Cell.Dev.Biol.-Animal

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The human HL-60 promyelocytic leukemia cell line has been widely used as a model for studying granulocytic differentiation. All-trans retinoic acid (ATRA) treatment of HL-60 cells promotes granulocytic differentiation and is effective as differentiation therapy for patients with acute promyelocytic leukemia. The identification of genes that are transcriptionally regulated by ATRA has provided insight into granulocytic differentiation and differentiation therapy. The Asb-2 (ankyrin repeat SOCS box 2) gene has previously been identified as a transcriptional target in ATRA-treated HL-60 cells. The ASB-2 protein forms an E3 ubiquitin ligase complex with the proteins, Cul5, regulator of cullin 2 (ROC2), and elongin B and C. The purpose of this study was to determine if there is increased expression of Cul5 during granulocytic differentiation of HL-60 cells. To induce granulocytic differentiation, HL-60 cells were treated for 5 d with ATRA and differentiation was confirmed by examining superoxide anion production, nuclear morphology, and changes in the expression of CD11b, CD13, and CD15. Quantitative real-time RT-PCR was used to measure Cul5 mRNA expression and also the expression of other components of the E3 ubiquitin ligase (ASB-2, ROC2, elongin B and C). Granulocytic differentiation of HL-60 cells was associated with a 1.6-, 1.7-, and 23-fold statistically significant (P

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“…In accordance with a previous study (Olins and Olins 2004), four nuclear morphologies were identified: ovoid ( Fig. 2A), indented (Fig.…”

Section: Resultssupporting

confidence: 84%

Section: Discussionmentioning

confidence: 60%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Granulocytic differentiation of HL-60 promyelocytic leukemia cells is associated with increased expression of Cul5

Baxter

Carlson

Mayer

et al. 2009

In Vitro Cell.Dev.Biol.-Animal

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“…We speculated that these changes in protein level caused nuclear lobulation and deformability of the granulocytic nuclei. [19][20][21][22][23][24]31,32 As can be seen in Fig. 2 and Supplemental Table 2, there is a dramatic difference in transcript level of LMNA between the 2 differentiated cell forms, with significant decrease following RA, versus a significant increase following TPA.…”

Section: Nuclear Envelope and Cytoskeletal Proteinsmentioning

confidence: 97%

Transcriptomes reflect the phenotypes of undifferentiated, granulocyte and macrophage forms of HL-60/S4 cells

Welch

Jauch

Langowski

et al. 2017

Nucleus

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To understand the chromatin changes underlying differential gene expression during induced differentiation of human leukemic HL-60/S4 cells, we conducted RNA-Seq analysis on quadruplicate cultures of undifferentiated, granulocytic-and macrophage-differentiated cell forms. More than half of mapped genes exhibited altered transcript levels in the differentiated cell forms. In general, more genes showed increased mRNA levels in the granulocytic form and in the macrophage form, than showed decreased levels. The majority of Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were significantly enriched in genes that exhibited differential transcript levels after either RA or TPA treatment. Changes in transcript levels for groups of genes with characteristic protein phenotypes, such as genes encoding cytoplasmic granular proteins, nuclear envelope and cytoskeletal proteins, cell adhesion proteins, and proteins involved in the cell cycle and apoptosis illustrate the profound differences among the various cell states. In addition to the transcriptome analyses, companion karyotyping by M-FISH of undifferentiated HL-60/S4 cells revealed a plethora of chromosome alterations, compared with normal human cells. The present mRNA profiling provides important information related to nuclear shape changes (e.g., granulocyte lobulation), deformability of the nuclear envelope and linkage between the nuclear envelope and cytoskeleton during induced myeloid chromatin differentiation.

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“…The LBR, the nuclear envelope lamins, the microtubules and the heterochromatin (and its epigenetic modifications) are essential for the nuclear assembly [4, 9, 12, 13]. Lobulation of neutrophil nuclei can be partially explained by a dose-dependent response to the amount of wild-type LBR.…”

Section: General Characteristicsmentioning

confidence: 99%

Pelger-Huët Anomaly: A Critical Review of the Literature

Speeckaert

Verhelst²,

Koch³

et al. 2009

Acta Haematol

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Pelger-Huët anomaly (PHA), an autosomal dominant haematological trait is characterised by neutrophil nuclear hypolobulation and modified chromatin distribution. Mutations in the lamin B receptor gene, a member of the sterol reductase family have been identified as the underlying cause. Due to its asymptomatic nature or lack of observer familiarity, PHA is often overlooked. In this review, we give an overview of the main pathophysiological, clinical, morphological and functional aspects of PHA. Furthermore, we highlight the importance of a comprehensive approach to the assessment of this laminopathy.

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Cytoskeletal influences on nuclear shape in granulocytic HL-60 cells

Cited by 86 publications

References 44 publications

Granulocytic differentiation of HL-60 promyelocytic leukemia cells is associated with increased expression of Cul5

Granulocytic differentiation of HL-60 promyelocytic leukemia cells is associated with increased expression of Cul5

Transcriptomes reflect the phenotypes of undifferentiated, granulocyte and macrophage forms of HL-60/S4 cells

Pelger-Huët Anomaly: A Critical Review of the Literature

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