Cytoskeletal proteins F-actin and β-dystrobrevin are altered by the cryopreservation process in bull sperm

Felipe-Pérez, Yazmín Elizabeth; Valencia, Jaime; Juárez-Mosqueda, Marı́a de Lourdes; Pescador, Nasario; Roa-Espitia, Ana Lilia; Hernández‐González, Enrique O.

doi:10.1016/j.cryobiol.2011.12.004

Cited by 20 publications

(15 citation statements)

References 64 publications

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“…PKC or PLC). In support of this, recent reports have shown that a number of cytoskeletal proteins can be altered by the osmotic stress during the cryopreservation process (Felipe‐Perez et al. 2011; Peña et al.…”

Section: Concluding Remarks and Future Directionsmentioning

confidence: 72%

The Membrane of the Mammalian Spermatozoa: Much More Than an Inert Envelope

Tapia

Macías‐García

Miró-Morán

et al. 2012

Reprod Domestic Animals

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Sperm plasma membrane is a very important structure that functions to protect sperm against extracellular injuries and to respond to physiological challenges. It plays a crucial role during sperm capacitation, in sperm-egg interaction and, finally, in fertilization. Concerning sperm technology, possibly the most important factors causing damage in mammalian spermatozoa membranes are initiated by the osmotic stress generated by dehydration of the cells during freezing and thawing. These changes are rapidly derived to the plasma and organelle membranes that gradually experiment loss of membrane architecture, causing unbalanced production of reactive oxygen species and increased lipid peroxidation. Other procedures such as sperm sorting or liquid storage of sperm also induce harmful changes in the integrity of the membrane. The specific composition of lipids of the sperm membranes may provide clues for understanding the mechanisms behind the differences found in the response to stress in different species. In the present review, we deal with the composition, architecture and organization of the sperm plasma membrane, emphasizing the factors that can affect membrane integrity. The intracellular signalling pathways related with membrane reorganization during capacitation and acrosome reaction are also reviewed.

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Section: Concluding Remarks and Future Directionsmentioning

confidence: 72%

The Membrane of the Mammalian Spermatozoa: Much More Than an Inert Envelope

Tapia

Macías‐García

Miró-Morán

et al. 2012

Reprod Domestic Animals

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“…The addition of glycerol as a cryoprotectant agent alters sperm functionality, mainly during the post-thawing sperm incubation stage [49], showing an increase in the proportion of epididymal spermatozoa with axonemal vacuoles, damaged plasma membranes, and abnormal mitochondria when compared to media without glycerol [50]. Furthermore, the cytoskeleton is responsible for the appropriate cell volume regulation and its stability is highly altered by the cryopreservation process, causing its proteins (e.g., F-actin) to become more fragile [13], [24], [51], [52]. Thus, in red deer, a higher volume of the sperm flagellum might result in an increased amount of ice crystals and increased formation of axonemal vacuoles during the freezing/thawing process, adversely affecting flagellum integrity and consequently, cell volume regulation and sperm velocity.…”

Section: Discussionmentioning

confidence: 99%

“…Numerous studies argue the implications of sperm flagellum in several biological processes such as sperm velocity, male reproductive success, relationships with testicle size, and spermatogenesis [16]–[21]. On the other hand, studies concerning the role played by flagellum size on sperm freezability have not yet been reported, although there is evidence of the fragility of its internal and external structures when they are exposed to the cryopreservation process [5], [7], [22]–[24].…”

Section: Introductionmentioning

confidence: 99%

Sperm Flagellum Volume Determines Freezability in Red Deer Spermatozoa

2014

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The factors affecting the inter-individual differences in sperm freezability is a major line of research in spermatology. Poor sperm freezability is mainly characterised by a low sperm velocity, which in turn is associated with low fertility rates in most animal species. Studies concerning the implications of sperm morphometry on freezability are quite limited, and most of them are based on sperm head size regardless of the structural parts of the flagellum, which provides sperm motility. Here, for the first time, we determined the volumes of the flagellum structures in fresh epididymal red deer spermatozoa using a stereological method under phase contrast microscopy. Sperm samples from thirty-three stags were frozen and classified as good freezers (GF) or bad freezers (BF) at two hours post-thawing using three sperm kinetic parameters which are strongly correlated with fertility in this species. Fourteen stags were clearly identified as GF, whereas nineteen were BF. No significant difference in sperm head size between the two groups was found. On the contrary, the GF exhibited a lower principal piece volume than the BF (6.13 µm3 vs 6.61 µm3, respectively, p = 0.006). The volume of the flagellum structures showed a strong negative relationship with post-thawing sperm velocity. For instance, the volume of the sperm principal piece was negatively correlated with sperm velocity at two hours post-thawing (r = −0.60; p<0.001). Our results clearly show that a higher volume of the sperm principal piece results in poor freezability, and highlights the key role of flagellum size in sperm cryopreservation success.

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“…Cryopreservation alters plasma membrane lipids from the liquid crystalline phase to the gel phase, called 'phase transition', an irreversible phenomenon (Quinn 1989), and causes loss of membrane proteins (Ollero et al 1998). In addition, cryopreservation adversely affects acrosomes (Jones and Stewart 1979;Anzar et al 2010), mitochondria (Jones and Stewart 1979;Courtens et al 1989;Martin et al 2004) and the cytoskeleton (Holt and North 1991;Felipe-Pérez et al 2012). Spermatozoa that survive cryopreservation exhibit premature capacitationlike changes (Cormier et al 1997;Cormier and Bailey 2003;Pons-Rejraji et al 2009) and are not fully competent to reach the fertilisation site and fertilise the oocyte (Watson 2000).…”

Section: Sperm Survival In Bull Semenmentioning

confidence: 99%

Sperm survival kinetics in different types of bull semen: progressive motility, plasma membrane integrity, acrosomal status and reactive oxygen species generation

Ahmad

Riaz

et al. 2015

Reprod. Fertil. Dev.

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This study was designed to compare the kinetics of sperm survival in different types of bull semen. Fresh ejaculates from four bulls were pooled, diluted in Tris-citric acid-egg yolk-glycerol extender, cooled to 4°C, frozen in LN2 and thawed at 37°C. Fresh, diluted, cooled and frozen-thawed semen were incubated at 37°C, and evaluated at 0, 2, 4, 6, 12 and 24h after the beginning of incubation. In Experiment 1, progressive sperm motility, normal acrosomes and plasma membrane integrity and asymmetry were determined. In Experiment 2, generation of superoxide anion (O2(•)) along with plasma membrane permeability and generation of hydrogen peroxide (H2O2) along with plasma membrane integrity were assessed. In Experiment 1, frozen-thawed semen had shorter survival times for progressive sperm motility, and spermatozoa with intact plasma membranes and acrosomes (IPM-IACR) as compared with other types of semen (P<0.05). Fresh spermatozoa underwent a necrotic pathway, diluted and cooled spermatozoa underwent an apoptosis-like pathway and frozen-thawed spermatozoa underwent both necrotic and apoptosis-like pathways. In Experiment 2, spermatozoa in all four types of semen exhibited O2(•-) generation and increased plasma membrane permeability, and became necrotic without H2O2 generation during incubation (P<0.05). In conclusion, frozen-thawed semen had shorter sperm longevity, which has important implications relating to the timing of artificial insemination. Different types of semen followed different death pathways. During incubation, spermatozoa in all types of semen generated O2(•-), which increased the permeability and compromised the integrity of the plasma membrane.

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Cytoskeletal proteins F-actin and β-dystrobrevin are altered by the cryopreservation process in bull sperm

Cited by 20 publications

References 64 publications

The Membrane of the Mammalian Spermatozoa: Much More Than an Inert Envelope

The Membrane of the Mammalian Spermatozoa: Much More Than an Inert Envelope

Sperm Flagellum Volume Determines Freezability in Red Deer Spermatozoa

Sperm survival kinetics in different types of bull semen: progressive motility, plasma membrane integrity, acrosomal status and reactive oxygen species generation

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