Cytosolic Free Calcium in Plasmodium falciparum-Infected Erythrocytes and the Effect of Verapamil: A Cytofluorometric Study

Adovelande, Jacques; Bastide, Bruno; Délèze, J.; Schrével, Joseph

doi:10.1006/expr.1993.1030

Cited by 51 publications

(31 citation statements)

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“…The latter result is in agreement with Adovelande (15). Since calcium ions play a central role in eukaryotic cells by regulating important cellular functions (16) it is crucial to understand how malaria parasites regulate their own calcium levels.…”

Section: Vol 42 No 5 1997supporting

confidence: 86%

Characterization of Ca2 transport activity associated with a non‐mitochondrial calcium pool in the rodent malaria parasite P. chabaudi

Passos

Garcia

1997

IUBMB Life

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SUMMARYNon-mitochondrial calcium deposits were investigated in the intraerythrocytic malaria parasite Plasmodium chabaudi at the trophozoite stage by means of arsenazo III in the presence of ATP and the mitochondfial poisons, antimycin and oligomycin . Addition of vanadate and 2,5-di-(t-butyl)-l,4-hydroquinone (BHQ), both known to interact with SERCA pump, induced calcium release by permeabilized parasites when the medium free calcium concentration was kept at 3.5 ~tM. The tumor promoter thapsigargin also caused elevation of the free calcium concentration in permeabilized parasites. Our results support the view that P. chabaudi sequesters calcium in an exchangeable form and maintains its calcium homeostasis by way of an endoplasmic reticulum Ca 2+ pump.

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Section: Vol 42 No 5 1997supporting

confidence: 86%

Characterization of Ca2 transport activity associated with a non‐mitochondrial calcium pool in the rodent malaria parasite P. chabaudi

Passos

Garcia

1997

IUBMB Life

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“…3). A similar result was reported for cytosolic free calcium using a cytofluorometric method (Adovelande et al 1993). The fraction of transglutaminase that seemed to be constitutively activated in the infected erythrocyte increased.…”

Section: Discussionsupporting

confidence: 85%

Increase of a Calcium Independent Transglutaminase Activity in the Erythrocyte during the Infection with Plasmodium falciparum

Wasserman

Márquez

Urquiza

et al. 1999

Mem. Inst. Oswaldo Cruz

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“…This could confound our objective of identifying compounds that disrupt the DV. Nevertheless, Ca 2ϩ dysfunction would still be detrimental to parasite development, as blocking of Ca 2ϩ oscillations has been shown to be lethal to the parasite (29,30). Despite this, we believe that DCB does indeed destabilize the DV membrane, as postscreening validation showed that DCB induces phenotypes reminiscent of PCD ( Fig.…”

Section: Discussionmentioning

confidence: 92%

A High-Content Phenotypic Screen Reveals the Disruptive Potency of Quinacrine and 3′,4′-Dichlorobenzamil on the Digestive Vacuole of Plasmodium falciparum

Lee

Goh

Ch'ng

et al. 2014

Antimicrob Agents Chemother

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Plasmodium falciparum is the etiological agent of malignant malaria and has been shown to exhibit features resembling programmed cell death. This is triggered upon treatment with low micromolar doses of chloroquine or other lysosomotrophic compounds and is associated with leakage of the digestive vacuole contents. In order to exploit this cell death pathway, we developed a high-content screening method to select compounds that can disrupt the parasite vacuole, as measured by the leakage of intravacuolar Ca 2؉ . This assay uses the ImageStream 100, an imaging-capable flow cytometer, to assess the distribution of the fluorescent calcium probe Fluo-4. We obtained two hits from a small library of 25 test compounds, quinacrine and 3=,4=-dichlorobenzamil. The ability of these compounds to permeabilize the digestive vacuole in laboratory strains and clinical isolates was validated by confocal microscopy. The hits could induce programmed cell death features in both chloroquine-sensitive and -resistant laboratory strains. Quinacrine was effective at inhibiting field isolates in a 48-h reinvasion assay regardless of artemisinin clearance status. We therefore present as proof of concept a phenotypic screening method with the potential to provide mechanistic insights to the activity of antimalarial drugs.

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Cytosolic Free Calcium in Plasmodium falciparum-Infected Erythrocytes and the Effect of Verapamil: A Cytofluorometric Study

Cited by 51 publications

References 0 publications

Characterization of Ca2 transport activity associated with a non‐mitochondrial calcium pool in the rodent malaria parasite P. chabaudi

Characterization of Ca2 transport activity associated with a non‐mitochondrial calcium pool in the rodent malaria parasite P. chabaudi

Increase of a Calcium Independent Transglutaminase Activity in the Erythrocyte during the Infection with Plasmodium falciparum

A High-Content Phenotypic Screen Reveals the Disruptive Potency of Quinacrine and 3′,4′-Dichlorobenzamil on the Digestive Vacuole of Plasmodium falciparum

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