Cytosporone B as a Biological Preservative: Purification, Fungicidal Activity and Mechanism of Action against Geotrichum citri-aurantii

Yin, Chang-Cheng; Liu, Hongxin; Shan, Yang; Gupta, Vijai Kumar; Jiang, Yueming; Zhang, Weiyang; Tan, Haibo; Gong, Liang

doi:10.3390/biom9040125

Cited by 15 publications

(5 citation statements)

References 44 publications

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“…Some strategies have been used to control of G. citri-aurantii , such as chlorine [ 7 ], gaseous ozone or ozonated water [ 8 ], exposures to elevated CO 2 concentration [ 9 ]. In addition, some inorganic salts which are incorporated in the generally recognized as safe compounds list (GRAS), has also been proved to be effective, the most important being sodium bicarbonate and potassium sorbate [ 1 ], the antagonists microorganism Bacillus subtilis [ 10 ], Muscodor albus [ 5 ], Rhodosporidium paludigenum , Cryptococcus laurentii [ 4 ], Pichia pastoris [ 11 ] and Cytosporone B [ 12 ], plant extract from Cistus villosus and Halimium antiatlanticum [ 13 ] and essential oils from plant species [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Thymol Encapsulated into HP-β-Cyclodextrin as an Alternative to Synthetic Fungicides to Induce Lemon Resistance against Sour Rot Decay

et al. 2020

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Consumers demand the use of eco-friendly fungicides to treat fruit and vegetables and governmental authorities have unauthorized the application of chemical antifungals for the efficient control of sour rot. In the present research, the microwave irradiation (MW) method was used to encapsulate thymol into 2-hydroxylpropyl-beta-cyclodextrin (HP-β-CD) and the effect of these HP-β-CD on controlling sour rot in citrus fruit, caused by Geotrichum citri-aurantii, was evaluated. Amounts of 25 and 50 mM of HP-β-CD-thymol were used, and compared with propiconazole, to control the decay of inoculated lemon fruit. The treatments were performed in curative and preventive experiments. The incidence and severity of Geotrichum citri-aurantii in 25 and 50 mM HP-β-CD-thymol-treated fruit were reduced in both experiments. The preventive 50 mM HP-β-CD-thymol treatment showed the best effect, reducing the sour rot, respiration rate and fruit weight loss during storage at 20 °C. HP-β-CD-thymol increased polyphenol concentration and the activity of antioxidant enzymes, such as catalase (CAT), ascorbate peroxidase (APX) and peroxidase (POD) in lemon peel, and the highest effects were found with the 50-mM dose. In conclusion, the results show that the use of thymol encapsulated by MW into HP-β-CD could be an effective and sustainable tool, a substitute to the synthetic fungicides, for G. citri-auriantii control in citrus fruit.

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Section: Introductionmentioning

confidence: 99%

Thymol Encapsulated into HP-β-Cyclodextrin as an Alternative to Synthetic Fungicides to Induce Lemon Resistance against Sour Rot Decay

et al. 2020

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“…and presenting a wide range of antitumor and antimicrobiotic activities—has a promising effect on the control of citrus decay caused by this pathogen, being comparable to that of fungicide prochloraz. Its mechanism of action is suggested to be related to the alteration of the morphology of pathogen cells, causing distortion of the mycelia and loss of membrane integrity [106].…”

Section: Alternative Control Methodsmentioning

confidence: 99%

Biological Control of Citrus Postharvest Phytopathogens

Bazioli

Belinato

Costa

et al. 2019

Toxins

116

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Citrus are vulnerable to the postharvest decay caused by Penicillium digitatum, Penicillium italicum, and Geotrichum citri-aurantii, which are responsible for the green mold, blue mold, and sour rot post-harvest disease, respectively. The widespread economic losses in citriculture caused by these phytopathogens are minimized with the use of synthetic fungicides such as imazalil, thiabendazole, pyrimethanil, and fludioxonil, which are mainly employed as control agents and may have harmful effects on human health and environment. To date, numerous non-chemical postharvest treatments have been investigated for the control of these pathogens. Several studies demonstrated that biological control using microbial antagonists and natural products can be effective in controlling postharvest diseases in citrus, as well as the most used commercial fungicides. Therefore, microbial agents represent a considerably safer and low toxicity alternative to synthetic fungicides. In the present review, these biological control strategies as alternative to the chemical fungicides are summarized here and new challenges regarding the development of shelf-stable formulated biocontrol products are also discussed.

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“…The main pathogens that infect citrus fruit include Penicillium digitatum, Penicillium italicum , and Geotrichum citri-aurantii , which cause green mold, blue mold, and sour rot, respectively [ 3 , 4 , 5 ]. The pathogens have seriously affected the yield and quality of citrus fruit, and the annual losses of citrus fruit due to pathogen infection are very large [ 6 , 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

Dimethyl Dicarbonate as a Food Additive Effectively Inhibits Geotrichum citri-aurantii of Citrus

Liu

Zhang

Wang

et al. 2022

Foods

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Dimethyl dicarbonate (DMDC), a food additive, can be added to a variety of foods as a preservative. This study aimed to evaluate the inhibitory effects of DMDC on Geotrichum citri-aurantii in vitro and in vivo, as well as the potential antifungal mechanism. In vitro experiments showed that 250 mg/L DMDC completely inhibited the growth of G. citri-aurantii and significantly inhibited spore germination by 96.33%. The relative conductivity and propidium iodide (PI) staining results showed that DMDC at 250 mg/L increased membrane permeability and damaged membrane integrity. Malondialdehyde (MDA) content and 2, 7-Dichlorodihydrofluorescein diacetate (DCHF-DA) staining determination indicated that DMDC resulted in intracellular reactive oxygen species (ROS) accumulation and lipid peroxidation. Scanning electron microscopy (SEM) analysis found that the mycelia were distorted and the surface collapsed after DMDC treatment. Morphological changes in mitochondria and the appearance of cavities were observed by transmission electron microscopy (TEM). In vivo, 500 mg/L DMDC and G. citri-aurantii were inoculated into the wounds of citrus. After 7 days of inoculation, DMDC significantly reduced the disease incidence and disease diameter of sour rot. The storage experiment showed that DMDC treatment did not affect the appearance and quality of fruits. In addition, we found that DMDC at 500 mg/L significantly increased the activity of citrus defense-related enzymes, including peroxidase (POD) and phenylalanine ammonia-lyase (PAL). Therefore, DMDC could be used as an effective method to control citrus sour rot.

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Cytosporone B as a Biological Preservative: Purification, Fungicidal Activity and Mechanism of Action against Geotrichum citri-aurantii

Cited by 15 publications

References 44 publications

Thymol Encapsulated into HP-β-Cyclodextrin as an Alternative to Synthetic Fungicides to Induce Lemon Resistance against Sour Rot Decay

Thymol Encapsulated into HP-β-Cyclodextrin as an Alternative to Synthetic Fungicides to Induce Lemon Resistance against Sour Rot Decay

Biological Control of Citrus Postharvest Phytopathogens

Dimethyl Dicarbonate as a Food Additive Effectively Inhibits Geotrichum citri-aurantii of Citrus

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