Cytostatic effect of gangliosides present in the membrane of macrophages

Ritter, Klaus; Härtl, Rolf; Bandlow, G.; Thomssen, R.

doi:10.1016/0008-8749(86)90395-3

Cited by 19 publications

(13 citation statements)

References 28 publications

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“…Cells of the monocyte lineage have been shown to induce growth arrest of immortalised cells [21]. The ganglioside IV 3 NeuAc-nLcOse 4 Cer was identi®ed to be the causing agent of this eect.…”

Section: Discussionmentioning

confidence: 99%

Enhanced transcription of the s-adenosylhomocysteine hydrolase gene precedes Epstein-Barr virus lytic gene activation in ganglioside-stimulated lymphoma cells

Schaade

Kleines

Krone

et al. 2000

Med Microbiol Immunol

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Stimulation of Epstein-Barr virus (EBV) genome-positive Burkitt lymphoma cells with the ganglioside IV3NeuAc-nLcOse4Cer leads to the induction of cell differentiation processes and activates the EBV lytic viral cycle. In cells of the Burkitt lymphoma line Raji differential expression of host cell genes was analysed in the early phase (150 min) post stimulation with the ganglioside to display the cell activities that precede the activation of the EBV lytic cycle using the differential display reverse transcription-polymerase chain reaction technique. Multiple fragment cDNAs derived from control cells and ganglioside-stimulated cells were amplified using random primers and displayed via polyacrylamide gel electrophoresis. The expression pattern of 8,400 bands was analysed. Eleven differentially expressed fragment cDNAs were reamplified and identified by nucleotide sequencing. Six of these could be identified as coding for proteins that may take part in virus reactivation and differentiation. The most striking finding was the induction of s-adenosylhomocysteine hydrolase (AHCY) expression. The cellular enzyme AHCY plays an important role in transmethylation reactions controlling the replication of several viruses. Thus. an involvement in EBV replication can be suggested.

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Section: Discussionmentioning

confidence: 99%

Enhanced transcription of the s-adenosylhomocysteine hydrolase gene precedes Epstein-Barr virus lytic gene activation in ganglioside-stimulated lymphoma cells

Schaade

Kleines

Krone

et al. 2000

Med Microbiol Immunol

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“…For their detection and identification the gangliosides were sprayed on silica gel 60 (alumium support; Merck, Darmstadt, Germany) forming a 5 rnm line; Linomat I11 (Camag, Mutenz, Switzerland) was employed. The chromatographic run was performed using the solvent chloroform : methanol : 0.02Y0 aqueous CaCI, (60 : 40 : 9, v/v/ v) for 50 min at room temperature (Ritter et al, 1985). The chromatogram was made visible by resorcinol-HC1 spray (Svennerholm, 1957) and heating at 110°C for 15 min.…”

Section: Methodsmentioning

confidence: 99%

Combined application of analytical high performance thin layer chromatography and electroblotting for the detection of anti‐ganglioside antibodies in human sera

Ritter

Schaade

Thomssen

et al. 1992

Biomedical Chromatography

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Antibodies against gangliosides isolated from small tumour and nervous tissue specimens can be reliably detected in serum samples by the ganglioside electrotransfer technique without the need for previous purification steps. After separation by high performance thin layer chromatography gangliosides are transferred from silica gel plates to hydrophobic polyvinylidene difluoride membranes. These membranes are highly suitable for immunostaining. The use of a 15-slit device allows simultaneous testing of up to 15 serum samples. Samples of serum from 39 patients with clear-cell carcinoma of the kidney, mammary carcinoma, ovarian carcinoma and neurological disorders together with samples from healthy controls were tested for anti-ganglioside antibodies from various tissues.

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“…The increase primarily is due to an elevation of GM3 [41] but in a number of cases (human melanomas, Hodgkin's disease, colon and uterus carcinoma) serum was found to contain appreciable amounts of GD3, which is much lower or absent in normal serum [13, 431. To what extent the higher circulating GM3 and GD3 levels in cancer patients actually are due to shedding from the tumor surface is not known and other sources may also be considered. It has, for example, been shown that macrophages release enhanced amounts of GD3 after stimulation [44].…”

Section: Hc -Ohmentioning

confidence: 99%