Cytotaxonomy of endangered species Orobanche filicicola in Korea and its closely related species, Orobanche coerulescens (Orobanchaceae) (I)

Choi, Bokyung; Jang, Tae‐Soo; Park, Jeong-Mi; Kim, Ji Hoon; Sim, Sunhee; Hyun, Chang Woo; Kim, Soonok; Park, Minsu; Na, Nu-Ree; Lee, Do Keun

doi:10.1016/j.japb.2020.04.001

Cited by 2 publications

(3 citation statements)

References 19 publications

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“…Aplikasinya banyak melibatkan bahan-bahan kimia dan fisika yang diberikan selama preparasi sampel. Adapun beberapa bahan kimia yang digunakan untuk meningkatkan kualitas preparate kroosom antara lain bromonaphthalene, p dichloro benzene, dan hidroksiquinolin (Choi et al, 2020). Hidroksikuinon pada penelitian ini diaplikasikan untuk membuat susunan akar tanaman cabai rawit.…”

Section: Pendahuluanunclassified

Analisis Kromosom Tanaman Cabai Rawit (Capsicum frustescens) Terhadap Lama Perendaman Hidroksiquinolin

Al-Amanah,

Ashar,

Fitri

et al. 2023

agrovital

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Penghitungan jumlah kromosom dapat dilakukan melalui penyelidikan kromosom dengan pra-perlakuan. Teknik untuk membuat pengaturan kromosom yang tepat untuk menciptakan partisi kromosom yang luas dan dapat dipahami, menjadikannya lebih mudah dalam proses penghitungan kromosom. Penelitian ini bertujuan untuk mengetahui pengaruh lama hidroksikuinolin terhadap sampel akar tanaman cabai rawit (Capsicum frutescens). Eksplorasi ini mencakup dua kegiatan yaitu perencanaan bahan dan pembuatan sampel preparat kromosom akar. Ada dua hal yang terlihat pada susunan kromosom akar cabai rawit, yaitu kemampuan mengisolasi kromosom dan ketajaman pewarnaan kromosom dibandingkan dengan sitoplasma di sekitarnya. Kesimpulan dari penelitian ini adalah jumlah kromosom cabai rawit adalah 2n=2x=24, pemeriksaan kromosom akar maristematik pada tanaman cabai rawit dengan memberikan pra-perlakuan dengan hidroksikuinolin selama 4 jam setelah fiksasi dapat menghasilkan pelepasan kromosom yang terbaik dibawah mikroskop lebih jelas dan memudahkan dalam penghitungan jumlah kromosom.

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Section: Pendahuluanunclassified

Analisis Kromosom Tanaman Cabai Rawit (Capsicum frustescens) Terhadap Lama Perendaman Hidroksiquinolin

Al-Amanah,

Ashar,

Fitri

et al. 2023

agrovital

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“…Squash preparations were made in a drop of 60% acetic acid. At least five well-spread chromosome plates were analyzed for each individual using a light microscope (Olympus BX-53, Tokyo, Japan) and photographed using a digital camera (Olympus DP-74, Tokyo, Japan), following the method by Choi et al [21]. A minimum of three well-spread chromosome plates was selected for karyotype analysis and chromosome-size measurement [23].…”

Section: Cytological Analysismentioning

confidence: 99%

“…Changes in chromosome number (aneuploidy/dysploidy or polyploidy) and structure (inversions, translocations, additions, deletions) play a crucial role in plant evolution and diversification [15][16][17]. Cytological research, including karyotype analysis and genome size estimation, is considered as an important and useful approach in evolutionary studies of angiosperms [18][19][20][21][22][23][24]. Previously published genome size values within genus Cypripedium showed a 10.83-fold difference, ranging from 4.14 pg/1C in C. molle to 44.84 pg/1C in C. acaule [24].…”

Section: Introductionmentioning

confidence: 99%

Cytological Study of Cypripedium japonicum Thunb. (Orchidaceae Juss.): An Endangered Species from Korea

Choi

Gang²,

Kim

et al. 2021

Plants

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Changes in chromosome number and karyotype evolution are important to plant diversification, as they are both major drivers of speciation processes. Herein, chromosome number, karyotype, and genome size of the Korean lady’s slipper orchid Cypripedium japonicum Thunb., an endangered species, were investigated in natural populations. Furthermore, all cytological data from this species are reported herein for the first time. The chromosome number of all investigated C. japonicum plants was diploid (2n = 2x = 22), with x = 11 as base chromosome number, whereby the species can now be clearly distinguished from the Japanese lady’s slipper orchid. The karyotypes of all studied individuals were of similar length, symmetrical, and rather unimodal. Flow cytometry of the C. japonicum revealed that the genome size ranged from 28.38 to 30.14 pg/1C. Data on chromosome number and karyotypes were largely consistent with previous results indicating that Korean (x = 11) populations of C. japonicum are more closely related to Chinese populations (x = 11) compared to Japanese (x = 10) populations. These comprehensive cytological results will benefit the efforts to discriminate the geographically isolated and endangered Eastern Asian (China, Japan, and Korea) lady’s slipper orchid species.

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Cytotaxonomy of endangered species Orobanche filicicola in Korea and its closely related species, Orobanche coerulescens (Orobanchaceae) (I)

Cited by 2 publications

References 19 publications

Analisis Kromosom Tanaman Cabai Rawit (Capsicum frustescens) Terhadap Lama Perendaman Hidroksiquinolin

Analisis Kromosom Tanaman Cabai Rawit (Capsicum frustescens) Terhadap Lama Perendaman Hidroksiquinolin

Cytological Study of Cypripedium japonicum Thunb. (Orchidaceae Juss.): An Endangered Species from Korea

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