Cytotoxic activities of acetoxyscirpenediol and ergosterol peroxide from Paecilomyces tenuipes

Induction of Apoptosis by Disturbing Mitochondrial-Membrane Potential and Cleaving PARP in Jurkat T Cells through Treatment with Acetoxyscirpenol Mycotoxins

“…As for 4-MAS cytotoxicity, the Jurkat T cell was much more sensitive than the other cell lines, such as the mouse sarcoma-180 or human SNU cancer cell lines. 16) Based on these results, it can be concluded that single acetoxylation at the C-4 position of the scirpenol family confers relatively weak cytotoxicity. On the other hand, acetoxylation at the C-15 position resulted in stronger cytotoxicity regardless of the pres- ence of other acetoxylations at the C-3 and/or C-4 position.…”

“…14) 4-MAS isolated from Isaria japonica induced apoptosis though caspase-3 activation and DNA frag-mentation. 15) Recently, we had reported the isolation of 4-MAS from Paecilomyces tenuipe, 16) which is an edible entomopathogenic fungus that infects an insect's body during summer, remains dormant throughout winter, and then forms a plant-like fruiting body during the following spring or summer. 17) In continuation of our efforts revealing action mechanisms of 4-MAS, the modulation of the cell-cycle-related gene expression in the MCF-7 cell line, 18) the inhibition of GLUT-1 in Xenopus laevis, 19) the inhibition of the sodium glucose co-transporter, 20) and cell-cycle arrest 21) had been reported.…”

4-Acetoxyscirpendiol of Paecilomyces tenuipes Inhibits Na+/D-Glucose Cotransporter Expressed in Xenopus laevis Oocytes

“…Following injection, oocytes were incubated in Barth Solution at 14 o C for 24 h before uptake and electrophysiological assays. Natural 4-ASD was isolated according to Nam et al (2001). Briefly, artificially cultivated conidophores of P. tenuipes were first extracted in methanol, and the extracts obtained were the concentrated approximately 10-fold at 45 o C under reduced pressure.…”

“…In particular, in China cordyceps is highly regarded as one of the most effective traditional medicines with anti-aging and general curative effects (Konno et al, 2001;Nam et al, 2001;Kiho et al, 2002;Kikuchi et al, 2004). Of the numerous effects exerted by cordyceps species like Paecilomyces tenuipes, the lowering of blood sugar content is probably considered to be the most interesting (Kiho et al, 1999;Talpur et al, 2002;Kikuchi et al, 2004).…”

“…An analysis of its chemical structure revealed that 4-ASD belongs to a class of trichothecenes. According to Nam et al (2001), 4-ASD induced significant levels of apoptosis in various human cancer cell lines. Thus, since the inhibition of small intestinal Na + /glucose co-transporter (SGLT-1) may help reduce blood-glucose concentrations, we investigated the effect of 4-ASD on its activity.…”