Cytotoxic Activities of Fractions from Dioscorea bulbifera L. Chloroform and Methanol Extracts on T47D Breast Cancer Cells

Nur, R M; Nugroho, Laurentius Hartanto

doi:10.5530/pj.2018.1.7

Cited by 11 publications

(9 citation statements)

References 10 publications

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“…High concentrations of the main compounds can be considered part of the plant's defense system (Hossain et al 2014). The plant extracts contain many metabolites and compounds, each of which is capable of acting synergistically or antagonistically, these compound components can have the maximum effect if they are in complex conditions along with other compound components (Virganita et al 2009;Nur and Nugroho 2018). The MIC in this fraction was 32 mg mL.…”

Section: Discussionmentioning

confidence: 98%

The potency of Sansevieria trifasciata and S. cylindrica leaves extracts as an antibacterial against Pseudomonas aeruginosa

et al. 2021

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Abstract. Nugroho LH, Dewatisari WF, Retnaningrum E, Purwestri YA. 2021. The potency of Sansevieria trifasciata and S. cylindrica leaves extracts as an antibacterial against Pseudomonas aeruginosa. Biodiversitas 22: 408-415. Sansevieria trifasciata and Sansevieria cylindrica are the major herbs in Indonesia, which contain several bioactive compounds as potential sources of antibacterial agents. This study aims to evaluate the antibacterial activity of S. trifasciata and S. cylindrica leaves extract and fraction against Pseudomonas aeruginosa and to identify its bioactive compounds. Crude ethanolic extract of S. trifasciata and S. cylindrica leaves were tested for their antimicrobial activity by disk diffusion method against P. aeruginosa. S. trifasciata showed strong antibacterial activity with an inhibition zone of 18.3 mm compared to S. cylindrica. Different concentrations of extract i.e. 4 mg/mL, 8 mg/mL, 16 mg/mL, 32 mg/mL, 64 mg/mL, 128 mg/mL and 256 mg/mL were tested for their minimum inhibitory concentrations (MIC). The MIC results showed that the S. trifasciata extract was able to inhibit bacterial growth at a concentration of 32 mg/mL. Results of vacuum liquid chromatography (VLC) and thin-layer chromatography (TLC) revealed that only fraction 3 showed the highest antibacterial activity at 16 mg/mL. In TLC bioautography analysis, fraction 3 showed a clear zone at Rf 0.93. The phytochemical analysis showed that terpenoid, phenolic, triterpenoid, and flavonoid compounds were found in S. trifasciata extract that were associated with antibacterial activity.

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Section: Discussionmentioning

confidence: 98%

The potency of Sansevieria trifasciata and S. cylindrica leaves extracts as an antibacterial against Pseudomonas aeruginosa

et al. 2021

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“…It was reported by Nur and Nugroho [ 32 ] that D. bulbifera extracts demonstrated a cytotoxic effect on T47D cell lines. This research result indicated that the chloroform extract of the D. bulbifera leaves was stronger in inhibiting the growth of the cells than the methanol extract.…”

Section: Discussionmentioning

confidence: 99%

Secondary Metabolites, Antioxidant, and Antiproliferative Activities of Dioscorea bulbifera Leaf Collected from Endau Rompin, Johor, Malaysia

Mainasara

Bakar

Akim

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Breast cancer is among the most commonly diagnosed cancer and the leading cause of cancer-related death among women globally. Malaysia is a country that is rich in medicinal plant species. Hence, this research aims to explore the secondary metabolites, antioxidant, and antiproliferative activities of Dioscorea bulbifera leaf collected from Endau Rompin, Johor, Malaysia. Antioxidant activity was assessed using 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), and 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) assays, while the cytotoxicity of D. bulbifera on MDA-MB-231 and MCF-7 breast cancer cell lines was tested using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Cell cycle analysis and apoptosis were assessed using flow cytometry analysis. Phytochemical profiling was conducted using gas chromatography-mass spectrometry (GC-MS). Results showed that methanol extract had the highest antioxidant activity in DPPH, FRAP, and ABTS assays, followed by ethyl acetate and hexane extracts. D. bulbifera tested against MDA-MB-231 and MCF-7 cell lines showed a pronounced cytotoxic effect with IC50 values of 8.96 μg/mL, 6.88 μg/mL, and 3.27 μg/mL in MCF-7 and 14.29 μg/mL, 11.86 μg/mL, and 7.23 μg/mL in MDA-MB-231, respectively. Cell cycle analysis also indicated that D. bulbifera prompted apoptosis at various stages, and a significant decrease in viable cells was detected within 24 h and substantially improved after 48 h and 72 h of treatment. Phytochemical profiling of methanol extract revealed the presence of 39 metabolites such as acetic acid, n-hexadecanoic acid, acetin, hexadecanoate, 7-tetradecenal, phytol, octadecanoic acid, cholesterol, palmitic acid, and linolenate. Hence, these findings concluded that D. bulbifera extract has promising anticancer and natural antioxidant agents. However, further study is needed to isolate the bioactive compounds and validate the effectiveness of this extract in the In in vivo model.

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“…We examined the effect of dalbergin on T47D cells via-bility using four complementary tests. In this experiment, we assessed T47D cell growth (24,25). Many polyphenols can decrease colony counts in estrogen receptor-positive human breast cancer cell lines (27).…”

Section: Discussionmentioning

confidence: 99%

Effect of a Neoflavonoid (Dalbergin) on T47D Breast Cancer Cell Line and mRNA Levels of p53, Bcl-2, and STAT3 Genes

Valojerdi

Goliaei

Parivar

et al. 2019

Iran Red Crescent Med J

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Background: Breast cancer is an important cause of death among women. Prevention of cancer through dietary intervention has recently received increasing interest. Lately, dietary polyphenols have gained much attention for their health benefits, including anticancer properties. Dalbergin as a polyphenol is synthesized from a common neoflavene intermediate. Objectives: This study aimed to examine whether dalbergin can be useful in the chemotherapy of estrogen receptor-positive T47D cell line. Methods: This experimental study was performed at the Laboratory of Biophysics and Molecular Biology,

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Cytotoxic Activities of Fractions from Dioscorea bulbifera L. Chloroform and Methanol Extracts on T47D Breast Cancer Cells

Cited by 11 publications

References 10 publications

The potency of Sansevieria trifasciata and S. cylindrica leaves extracts as an antibacterial against Pseudomonas aeruginosa

The potency of Sansevieria trifasciata and S. cylindrica leaves extracts as an antibacterial against Pseudomonas aeruginosa

Secondary Metabolites, Antioxidant, and Antiproliferative Activities of Dioscorea bulbifera Leaf Collected from Endau Rompin, Johor, Malaysia

Effect of a Neoflavonoid (Dalbergin) on T47D Breast Cancer Cell Line and mRNA Levels of p53, Bcl-2, and STAT3 Genes

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