Cytotoxic activities of some Greek Labiatae herbs

Abstract

Nineteen methanolic crude plant extracts of Labiatae family, collected in Greece from different locations, were evaluated for cytotoxic activity against brine shrimps and three human cancer cell lines along with a normal mouse cells as a control cell line. In the brine shrimp lethality test, Mentha pulegium was the only sample found to be active with an LC(50) value 347.3 micro g/ml, while all remaining samples had LC(50) values greater than 1000 micro g/ml. In case of Caco-2 and HepG2 cell lines, only one sample, namely Thymus parnassicus Halacsy, was active with LC(50) values 44.6 and 50.3 micro g/ml respectively, while against MCF-7 cell line, two samples, namely, Clinopodium vulgare L. (LC(50): 60.4 micro g/ml), and Thymus parnassicus Halacsy (LC(50): 54.7 micro g/ml), were found active.

Suppression of lipopolysaccharide-induced inflammatory responses in RAW 264.7 murine macrophages by aqueous extract of Clinopodium vulgare L. (Lamiaceae)

“…Experimental evidence corroborates the antibacterial (Opalchenova and Obreshkova, 1999) and antitumor activity (Dzhambazov et al, 2002;Badisa et al, 2003) of the plant. In contrast, studies supporting the traditional use of the wild basil for its antioxidant and antiphlogistic properties are scarce.…”

Modulation of microglia polarization dynamics during diabetic retinopathy in db / db mice

“…The cells were then treated with R-DS-ONJ (1-50 μM) for 24 h. Following incubation, the viability of the cells was measured with crystal violet as described [25].…”

Mitigative action of monoisoamyl-2,3-dimercaptosuccinate (MiADMS) against cadmium-induced damage in cultured rat normal liver cells

“…At the end of the incubation period, the viability of the cells was evaluated by dye uptake assay according to Badisa et al (2003). After 24 h of treatment, glutaraldehyde (400 μl of 0.25%) was added to each well to give 0.07% final concentration and incubated for 30 min at room temperature to fix the viable cells.…”