Cytotoxic activity assay from leaves and fruit extracts of <i>Ficus aurata</i> (Miq.) using brine shrimp lethality test method

Nurhamidah,; Nurdin, Hazli; Manjang, Yunazar; Dharma, Abdi; Suryati,

doi:10.1088/1742-6596/1116/4/042028

Cited by 2 publications

(2 citation statements)

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“…Metode Brine Shrimp Lethality Test (BSLT) pertama kali dilakukan oleh Meyer, dkk dan dapat digunakan sebagai petunjuk untuk senyawa sitotoksik, antiparasit, dan insektisida. Pada pengujian aktivitas dengan metode BSLT digunakan larva Artemia salina [7].…”

Section: Pendahuluanunclassified

Isolasi dan Karakterisasi Senyawa Triterpenoid dari Ekstrak Etil Asetat Daun Salam (Syzygium polyanthum (Wight) Walp.) serta Uji Sitotoksik Menggunakan Metode BSLT (Brine Shrimp Lethality Test)

Ferdinal,

Muhamad Fadli,

Rizki Septiardi

et al. 2023

J. Kim. Unand

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The bay plant (Syzygium polyanthum (Wight) Walp.) is a member of the myrtaceae family. The most widely used part of the bay plant is the bay leaf. The leaves of this plant are often used as traditional medicine to treat gout, diarrhea, diabetes, and high blood pressure. This study aims to isolate and determine the characteristics of triterpenoid compounds from ethyl acetate extract of bay leaves. Isolation was carried out by column chromatography method with SGP (Step Gradient Polarity) elution system, then the isolated compound was purified by recrystallization method and obtained in the form of white crystals weighing 0.0188 g. TLC results showed a single purple spot after being treated with Liebermann Buschard reagent with a melting point range of 137-139 oC. The results of the UV spectrum data showed no conjugated double bonds and the results of the FTIR spectrum data showed the presence of hydroxyl groups, C-O alcohols, C-H alkanes, C=C alkenes, geminal dimethyl which are characteristic of triterpenoid compounds. Cytotoxic activity testing was carried out using the Brine Shrimp Lethality Test (BSLT) method. Ethyl acetate extract and methanol extract had strong cytotoxic activity with LC50 values 316.23 mg/L and 234.42 mg/L respectively, while the hexane extract was weak with an LC50 value of 1148.15 mg/L.

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Section: Pendahuluanunclassified

Isolasi dan Karakterisasi Senyawa Triterpenoid dari Ekstrak Etil Asetat Daun Salam (Syzygium polyanthum (Wight) Walp.) serta Uji Sitotoksik Menggunakan Metode BSLT (Brine Shrimp Lethality Test)

Ferdinal,

Muhamad Fadli,

Rizki Septiardi

et al. 2023

J. Kim. Unand

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“…As mention before, 3,4-dihydroxybenzoic acid was determined as a phenolic compound. According to Lizárraga-Velázquez et al (2020), the mechanism of antibacterial activity of phenolic compounds is related to the damage of the bacteria cell membrane, which is process involves modifying the membrane permeability leading to the loss of cell wall integrity and changes in intracellular functions. While, β-sitosterol is one of phytosterols which the structure similar to the cholesterol and represent a diverse group of triterpenes (Gylling and Simonen, 2015;Uddin et al, 2018).…”

Section: Antibacterial Activity Of the Isolated Compoundsmentioning

confidence: 99%

Antibacterial activity of secondary metabolites isolated from Ficus aurata (Miq.) fruits

Andriani

Nurhamidah

Lutfi

et al. 2021

CRBB

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This research aimed to isolate chemical constituents from ethyl acetate and n-hexane fractions of Ficus aurata (Miq.) fruits and investigated their antibacterial activities. Chemical constituents were separated by using vacuum column chromatography, gravity column chromatography, and thin layer chromatography. The structures of the isolated compounds 3,4-dihydroxybenzoic acid (1) and β-sitosterol (2) were elucidated using various spectroscopic methods, including UV, IR, and NMR (1H-NMR, 13C-NMR, and DEPT). While, antibacterial activity was done by assay using Resazurin Microtiter Assay method. These 2 compounds reported as new constituents from F. aurata fruit. In addition, analysis of the bioassay showed that compound 1 had a notable antibacterial activity against gram negative (Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATTC 27853), and gram-positive bacteria (Staphylococcus aureusATCC 23235) with the Minimum Inhibitory Concentration (MIC) value of 0.1563 µg/mL, and it indicated the same MIC as cefadroxil (positive control). Compound 2 has weak antibacterial activity compared to cefadroxil (positive control). The results found that Ficus aurata (Miq.) fruits metabolites have antibacterial activity and show potency as antibacterial agent. Different kinds of bacteria and assay could be needed to investigate and support its antibacterial activity in the future.

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Cytotoxic activity assay from leaves and fruit extracts of Ficus aurata (Miq.) using brine shrimp lethality test method

Cited by 2 publications

References 1 publication

Isolasi dan Karakterisasi Senyawa Triterpenoid dari Ekstrak Etil Asetat Daun Salam (Syzygium polyanthum (Wight) Walp.) serta Uji Sitotoksik Menggunakan Metode BSLT (Brine Shrimp Lethality Test)

Isolasi dan Karakterisasi Senyawa Triterpenoid dari Ekstrak Etil Asetat Daun Salam (Syzygium polyanthum (Wight) Walp.) serta Uji Sitotoksik Menggunakan Metode BSLT (Brine Shrimp Lethality Test)

Antibacterial activity of secondary metabolites isolated from Ficus aurata (Miq.) fruits

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