Cytotoxic Activity of Aporphine Alkaloids from Alseodaphne Peduncularis (Wall. Ex. Ness) Meissn against Hela Cell Line

Amna, Ulil; Ahmad, Kafeel; Nafiah, Mohd Azlan

doi:10.22373/crc.v1i2.769

Cited by 3 publications

(3 citation statements)

References 8 publications

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“…Vincristine, a conventional drug of cancer, was used as a positive control against HeLa cell line and showed CD 50 value of 0.4 μg/ml. [24] The cytotoxicity of the extracts was assayed at various concentrations of 1000, 500, 100, 50, 20, 10, 5, and 1 μg/ml under continuous exposure for 72 h, which are expressed in CD 50 values and are summarized in Table 2.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of cytotoxic activity from Temurui (Murraya koenigii [Linn.] Spreng) leaf extracts against HeLa cell line using MTT assay

Amna

Halimatussakdiah

Wahyuningsih

et al. 2019

J Adv Pharm Technol Res

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Temurui ( Murraya koenigii [Linn.] Spreng) is a local plant of Aceh. The leaves of M. koenigii are used in most of the local foods as spices. Nowadays, cancer is claimed as the second deadly disease in the world where the number of sufferers increases every year. Cervical cancer (HeLa) is one of the most dominant cancers that happen in developing country, including Indonesia. Some chemotherapeutic agents using synthetic drugs have been used to treat cancer, but they are relatively expensive and cause poisoning that limits their use. Based on literatures, M. koenigii serves the potential secondary metabolites that could be developed as anticancer agent. This study aims to evaluate the cytotoxic activity from three extracts of M. koenigii leaves against HeLa cell line, including hexane, ethyl acetate, and methanol. Methodology used included extraction process, phytochemical screening, and cytotoxicity evaluation. The result showed that all the three extracts demonstrated a potent cytotoxic activity for HeLa cancer cells. Hexane and ethyl acetate showed a very strong cytotoxic effect with CD 50 values <1 μg/mL, whereas methanol extract showed cytotoxic effect with CD 50 value of 2.25 μg/mL. These results prove the potential of M. koenigii as an anticancer agent.

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Section: Resultsmentioning

confidence: 99%

Evaluation of cytotoxic activity from Temurui (Murraya koenigii [Linn.] Spreng) leaf extracts against HeLa cell line using MTT assay

Amna

Halimatussakdiah

Wahyuningsih

et al. 2019

J Adv Pharm Technol Res

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“…Recently, Amna et al . () observed that laurotetanine showed very potent activity against heLa cells in vitro but none of the four aporphinoid alkaloids they tested had any effect against normal mouse fibroblast (NIH/3T3) cell lines according to the 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐ diphenyltetrazolium bromide (MTT) assay. Some aporphinoids also possess a vasodilator effect (by inhibition of extracellular calcium entry and sometimes tissue‐specific modifications of intracellular calcium movements) and anti‐platelet activity.…”

Section: Introductionmentioning

confidence: 99%

“…Anticancer activities in vivo have been reported in few cases and the dimeric molecule thaliblastine had been advanced to human clinical trials but these were halted due to no significant therapeutic activity being observed (St evigny et al 2005). Recently, Amna et al (2017) observed that laurotetanine showed very potent activity against heLa cells in vitro but none of the four aporphinoid alkaloids they tested had any effect against normal mouse fibroblast (NIH/3T3) cell lines according to the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Some aporphinoids also possess a vasodilator effect (by inhibition of extracellular calcium entry and sometimes tissue-specific modifications of intracellular calcium movements) and anti-platelet activity.…”

Section: Introductionmentioning

confidence: 99%

Aporphinoid alkaloids as antimicrobial agents against Yersinia enterocolitica

Marco

Estrada

Pungitore

2019

Lett Appl Microbiol

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Foodborne diseases have become a health issue worldwide, mainly due to the consumption of contaminated foods that are either raw, improperly heat treated or cross‐contaminated after adequate heat treatment foods. A group of alkaloids extracted from plants were tested to evaluate their antimicrobial effect against different strains of Yersinia enterocolitica and other foodborne bacteria. The results obtained reveal that oliveridine and pachypodanthine inhibited Y. enterocolitica growth, with MIC values of 25 μmol l−1 and 100 μmol l−1 respectively. The results indicated that both alkaloids are good growth inhibitors, but oliveridine showed greater inhibitory effect with lower MIC values. Inhibitory alkaloids can be developed as potential antimicrobials in food system to prevent or treat foodborne diseases, thus contributing to solve the global issue of contaminated food consumption. Significance and Impact of the Study Alkaloids are abundant secondary metabolites in plants and represent one of the most widespread class of compounds endowed with multiple and varied pharmacological properties. In this work, we propose two aporphinoid alkaloids extracted from plants as new antimicrobial agents. Oliveridine and pachypodanthine inhibited Yersinia enterocolitica growth for up to 96 h of culture. This is the first reported study of the activity of these alkaloids as antimicrobial compounds.

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Toxicity of Malaysian Medicinal Plant Extracts Against Sitophilus oryzae and Rhyzopertha dominica

Hematpoor¹,

Liew²,

Omar³

et al. 2022

JTAS

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The insecticidal activities of extracts from 22 Malaysian medicinal plant extracts from 8 botanical families were tested against rice weevil: Sitophilus oryzae (L.) and lesser grain borer: Rhyzopertha dominica (F.). The extracts were obtained using hexane, methanol, and dichloromethane to extract potential biopesticides from dried leaves. The toxicity levels were examined periodically based on antifeedant activity and contact toxicity assays using treated grain assay. Hexane extracts of Alpinia conchigera, Alpinia scabra, Curcuma mangga, Curcuma purpurascens, Goniothalamus tapisoides, Piper sarmentosum, and methanol extracts of Curcuma aeruginosa, C. mangga, and Mitragyna speciosa were the most potent extracts against S. oryzae and R. dominica with lethal concentration (LC50) values of ≤ 0.42 mg/mL and ≤ 0.49 mg/mL, respectively. The contact toxicity test results showed that methanol extracts of C. aeruginosa and C. mangga, dichloromethane extracts of Cryptocarya nigra, and hexane extracts of C. mangga, and C. purpurascens resulted in 100% mortality of both pests within 28 days exposure of 5 mg/cm2 concentration.

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Cytotoxic Activity of Aporphine Alkaloids from Alseodaphne Peduncularis (Wall. Ex. Ness) Meissn against Hela Cell Line

Cited by 3 publications

References 8 publications

Evaluation of cytotoxic activity from Temurui (Murraya koenigii [Linn.] Spreng) leaf extracts against HeLa cell line using MTT assay

Evaluation of cytotoxic activity from Temurui (Murraya koenigii [Linn.] Spreng) leaf extracts against HeLa cell line using MTT assay

Aporphinoid alkaloids as antimicrobial agents against Yersinia enterocolitica

Toxicity of Malaysian Medicinal Plant Extracts Against Sitophilus oryzae and Rhyzopertha dominica

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