Cytotoxic activity of clinical Stenotrophomonas maltophilia

Figueiredo, Patrícia Maria; Furumura, M.T.; Santos, Aline Mara dos; Sousa, Ana Carolina T. de; Kota, D.J.; Levy, Carlos Emílio; Yano, Tomomasa

doi:10.1111/j.1472-765x.2006.01965.x

Cited by 47 publications

(40 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Given the conservation of xps genes among four other S. maltophilia genomes, we infer that Xps T2S is active in other strains of S. maltophilia. In support of this hypothesis, we along with other investigators have found protease activities in supernatants from a variety of other strains of S. maltophilia (3,20,23,45,46). The ϳ47-kDa protein that we observed in the K279a supernatant but not in the xps mutants' supernatants is likely to be StmPr1, a 47-kDa serine protease whose gene sequence encodes a signal sequence (23).…”

Section: Discussionsupporting

confidence: 72%

“…The detrimental effects were cell rounding, actin rearrangement, and cell detachment within a 3-h time period as well as cell death after 24 h. Compatible with these data are two past studies that reported that supernatants from other S. maltophilia strains elicit rounding and death of HEp-2 (human larynx), HeLa (human cervix), and Vero (African green monkey) cells as well as rounding and detachment of human fibroblasts (23,45). Though it is tempting to speculate that the protease activities that we identified in K279a supernatants are responsible for the effects on A549 cells, one of the previous studies found that the toxic activity, though sensitive to heat (56°C) treatment, was resistant to protease inhibitors, including those that impede serine proteases (45). On the one hand, it is possible that the different effects on A549 cells that we observed are interconnected and due to a single secreted protein.…”

Section: Discussionmentioning

confidence: 55%

See 1 more Smart Citation

Stenotrophomonas maltophilia Encodes a Type II Protein Secretion System That Promotes Detrimental Effects on Lung Epithelial Cells

2013

View full text Add to dashboard Cite

The Gram-negative bacterium Stenotrophomonas maltophilia is increasingly identified as a multidrug-resistant pathogen, being associated with pneumonia, among other infections. Despite this increasing clinical problem, the genetic and molecular basis of S. maltophilia virulence is quite minimally defined. We now report that strain K279a, the first clinical isolate of S. maltophilia to be sequenced, encodes a functional type II protein secretion (T2S) system. Indeed, mutants of K279a that contain a mutation in the xps locus exhibit a loss of at least seven secreted proteins and three proteolytic activities. Unlike culture supernatants from the parental K279a, supernatants from multiple xps mutants also failed to induce the rounding, detachment, and death of A549 cells, a human lung epithelial cell line. Supernatants of the xps mutants were also unable to trigger a massive rearrangement in the host cell's actin cytoskeleton that was associated with K279a secretion. In all assays, a complemented xpsF mutant behaved as the wild type did, demonstrating that Xps T2S is required for optimal protein secretion and the detrimental effects on host cells. The activities that were defined as being Xps dependent in K279a were evident among other respiratory isolates of S. maltophilia. Utilizing a similar type of genetic analysis, we found that a second T2S system (Gsp) encoded by the K279a genome is cryptic under all of the conditions tested. Overall, this study represents the first examination of T2S in S. maltophilia, and the data obtained indicate that Xps T2S likely plays an important role in S. maltophilia pathogenesis.

show abstract

Section: Discussionsupporting

confidence: 72%

Section: Discussionmentioning

confidence: 55%

Stenotrophomonas maltophilia Encodes a Type II Protein Secretion System That Promotes Detrimental Effects on Lung Epithelial Cells

2013

View full text Add to dashboard Cite

show abstract

“…Clinical S. maltophilia isolates have been reported to demonstrate cytotoxicity activity (111). Supernatants of some clinical S. maltophilia isolates recovered from liver and trachea exhibited hemolytic and enzymatic activities.…”

Section: Hydrolytic Enzymesmentioning

confidence: 99%

“…These effects included rounding, membrane blebbing, a loss of intercellular junctions, and cell death after 24 h. The tested protease inhibitors failed to inhibit the cytotoxic activity of the S. maltophilia isolates. In addition to the hemolytic and cytotoxic activities, these S. maltophilia isolates demonstrated additional virulence factors, including protease, lipase, and lecithinase activities, while isolates recovered from blood did not demonstrate any of these virulence factors or hemolytic and cytotoxic activities (111).…”

Section: Hydrolytic Enzymesmentioning

confidence: 99%

Stenotrophomonas maltophilia: an Emerging Global Opportunistic Pathogen

2012

View full text Add to dashboard Cite

SUMMARY Stenotrophomonas maltophilia is an emerging multidrug-resistant global opportunistic pathogen. The increasing incidence of nosocomial and community-acquired S. maltophilia infections is of particular concern for immunocompromised individuals, as this bacterial pathogen is associated with a significant fatality/case ratio. S. maltophilia is an environmental bacterium found in aqueous habitats, including plant rhizospheres, animals, foods, and water sources. Infections of S. maltophilia can occur in a range of organs and tissues; the organism is commonly found in respiratory tract infections. This review summarizes the current literature and presents S. maltophilia as an organism with various molecular mechanisms used for colonization and infection. S. maltophilia can be recovered from polymicrobial infections, most notably from the respiratory tract of cystic fibrosis patients, as a cocolonizer with Pseudomonas aeruginosa . Recent evidence of cell-cell communication between these pathogens has implications for the development of novel pharmacological therapies. Animal models of S. maltophilia infection have provided useful information about the type of host immune response induced by this opportunistic pathogen. Current and emerging treatments for patients infected with S. maltophilia are discussed.

show abstract

“…(19). The extracellular protease StmPrl has been recently shown to be relevant for the pathogenicity of S maltophilia (13).…”

Section: Molecular Detection Ofvirulence Related Genesmentioning

confidence: 99%

Molecular Characterization of Virulence Determinants of Stenotrophomonas Maltophilia Strains Isolated from Patients Affected by Cystic Fibrosis

Bonaventura

Prosseda

Chierico

et al. 2007

Int J Immunopathol Pharmacol

View full text Add to dashboard Cite

Stenotrophomonas maltophilia is an emerging nosocomial bacterial pathogen which is currently isolated with increasing frequency from the airways of cystic fibrosis (CF) patients. In this study 13 S. maltophilia strains (11 isolated from the airways of independent CF patients, and two non-CF respiratory reference strains) have been characterized for the expression of several virulence-associated factors. In particular, the ability to form biofilm on abiotic surfaces has been determined and correlated with different features, such as motility, adherence and the ability to invade A549 respiratory epithelial cells. Moreover, the presence of a flagellum-associated gene as well as that of the StmPr1 gene, which encodes an extra-cellular protease, have been determined by Southern blot hybridization. Our data indicate that the different degree of biofilm formation exhibited by the 11 CF isolates does not correlate with motility, ability to adhere to and invade A549 cells, or with the presence of flagella. On the other hand, among the CF isolates the StmPr1 gene was found only in two strains, both able to establish chronic lung infections in CF patients. Moreover, only four of the strains analyzed show a temperature-independent antibiotic-resistance profile, suggesting either a different origin of these strains or an intervening adaptation to host tissues.

show abstract

Cytotoxic activity of clinical Stenotrophomonas maltophilia

Cited by 47 publications

References 28 publications

Stenotrophomonas maltophilia Encodes a Type II Protein Secretion System That Promotes Detrimental Effects on Lung Epithelial Cells

Stenotrophomonas maltophilia Encodes a Type II Protein Secretion System That Promotes Detrimental Effects on Lung Epithelial Cells

Stenotrophomonas maltophilia: an Emerging Global Opportunistic Pathogen

Molecular Characterization of Virulence Determinants of Stenotrophomonas Maltophilia Strains Isolated from Patients Affected by Cystic Fibrosis

Contact Info

Product

Resources

About