Cytotoxic activity of crude extracts and fractions from Premna odorata (Blanco), Artocarpus camansi (Blanco) and Gliricidia sepium (Jacq.) against selected human cancer cell lines

Tantengco, Ourlad Alzeus G.; Jacinto, Sonia D.

doi:10.1016/j.apjtb.2015.09.011

Cited by 23 publications

(15 citation statements)

References 17 publications

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“…Generally, the crude methanol extracts showed higher toxicity than the individual fractions since it contains all the cytotoxic components. This is in line with previous reports where cytotoxicity of crude extracts and fractions have been evaluated (Rasoanaivo et al, 2011;Tantengco and Jacinto, 2015;Mtunzi et al, 2017).…”

Section: Discussionsupporting

confidence: 92%

Cytotoxicity of selected Ethiopian medicinal plants used in traditional breast cancer treatment against breast-derived cell lines

Tuasha¹,

Seifu²,

Gadisa³

et al. 2019

J. Med. Plants Res.

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Traditional medicine is widely practiced in Ethiopia. Here we investigate the toxicity of extracts of seven medicinal plants traditionally used to treat breast cancer in Ethiopia. These plants, Sideroxylon oxyacanthum, Zanthoxylum chalybeum, Clematis simensis, Clematis longicauda, Dovyalis abyssinica, Vernonia leopoldi, and Clerodendrum myricoides, were selected based on recommendations by traditional healers and on the frequency of use. After harvesting the plant material, the water content was determined and the powder was subjected to methanol extraction resulting in crude extracts which were tested for cytotoxicity in dose response assay. Then the methanol extract of the most toxic plants was subjected to further solvent-solvent fractionation to gain petroleum ether, hexane, chloroform, ethyl acetate, and water fractions and these were also tested for cytotoxicity in dose response assays. Extracts of Z. chalybeum and C. myricoides were not toxic. The crude extracts of S. oxyacanthum, C. simensis, and D. abyssinica showed cytotoxicity with half maximal inhibitory concentration 50% (IC50) below 1 μg/ml in the human breast cancer cell lines JIMT-1, MCF-7, and HCC1937. The ethyl acetate fraction of V. leopoldi was the most cytotoxic fraction of all fractions tested with an IC50 of 0.87 μg/ml in JIMT-1 cells. The aqueous fraction of S. oxyacanthum and the chloroform fraction of C. simensis were also cytotoxic. In conclusion, our data show a wide difference in in vitro toxicity of medicinal plants used to treat breast cancer patients, which may guide the use of traditional medicine and the choice of plants for isolation of new compounds for cancer treatment.

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Section: Discussionsupporting

confidence: 92%

Cytotoxicity of selected Ethiopian medicinal plants used in traditional breast cancer treatment against breast-derived cell lines

Tuasha¹,

Seifu²,

Gadisa³

et al. 2019

J. Med. Plants Res.

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“…Each concentration of treated cells, untreated cells and blank was performed in triplicate in one plate and the experiment was repeated for three times for validity. Seventy two hours treatment time was chosen which is in line with a number of previous experiment, that the treatment effect was to be done in a time dependent manner [ 19 – 21 ]. Therefore, following a 72-h incubation period at 37 °C with 5% CO 2 in incubator, 20 μl of MTT solution was added to wells and incubated for an additional 4 h. Medium of each well was carefully aspirated without disturbing MTT crystal in each well.…”

Section: Methodsmentioning

confidence: 99%

In vitro investigation of cytotoxic and antioxidative activities of Ardisia crispa against breast cancer cell lines, MCF-7 and MDA-MB-231

Nordin

Kadir

Zakaria

et al. 2018

BMC Complement Altern Med

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BackgroundArdisia crispa Thunb. D.C is used mostly in some parts of the Asian region by traditional practitioners to treat certain diseases associated with oxidative stress and inflammation including cancer and rheumatism. In Malaysia, it is popularly known as ‘Mata Ayam’ and local traditional practitioners believed that the root of the plant is therapeutically beneficial.MethodsThe cytotoxic effect of hydromethanolic extract of A. crispa and its solvents partitions (ethyl acetate and aqueous extracts) against breast cancer cells were evaluated by using MTT assay. The cells were treated with concentration of extracts ranging from 15.63 μg/mL- 1000 μg/mL for 72 h. The quantification of phenolic and flavonoid contents of the extracts were carried out to determine the relationship between of phytochemical compounds responsible for cytotoxic and antioxidative activities. The antioxidant capacity was measured by DPPH and ABTS free radical scavenging assay and expressed as milligram (mg) Trolox equivalent antioxidant capacity per 1 g (g) of tested extract.ResultsThe hydromethanolic and ethyl acetate extracts showed moderate cytotoxic effect against MCF-7 with IC50 values of 57.35 ± 19.33 μg/mL, and 54.98 ± 14.10 μg/mL, respectively but aqueous extract was inactive against MCF-7. For MDA-MB-231, hydromethanolic, ethyl acetate and aqueous extracts exhibited weak cytotoxic effects against MDA-MB-231 with IC50 values more than 100 μg/mL. The plant revealed high total phenolic content, total flavonoid and antioxidant capacity.ConclusionThe response of different type of breast cancer cell lines towards A. crispa extract and its partitions varied. Accordingly, hydromethanolic and ethyl acetate extracts appear to be more cytotoxic to oestrogen receptor (ER) positive breast cancer than oestrogen receptor (ER) negative breast cancer. However, aqueous extract appears to have poor activity to both types of breast cancer. Besides that, hydromethanolic and ethyl acetate extracts exhibit higher TPC, TFC and antioxidant capacity compared to aqueous extract. Synergistic effect of anticancer and antioxidant bioactives compounds of A. crispa plausibly contributed to the cytotoxic effects of the extract.

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“…Likewise, a study on the alcohol extract of the plant showed significant antitumor activity 33 while another study showed the leaves and bark hexane fractions to be highly cytotoxic against the HCT116, MCF-7 and A549 cancer cell lines although it was shown the bark hexane extract has highest selectivity index for the three cancer lines 39 . This current study show C. ovatum ethanolic extracts of the leaves was having high antioxidant properties and high toxicity to HCT116, A549 and AA8 cell lines.…”

Section: Discussionmentioning

confidence: 99%

“…Only the species T. copelandii investigated in this study is popular as timber and only reported through the word of mouth that are also used for treating basic ailments by the local people. P. odorata is reported to have sudorific, pectoral, carminative, antimicrobial, cardiotonic, anticoagulant, hepatoprotective, antitubercular, antitumor properties [30][31][32][33][34][35][36][37][38][39] .…”

Section: Ijpsr (2018) Volume 9 Issue 11 (Research Article)mentioning

confidence: 99%

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2018

IJPSR

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Chemotherapy and radiation are the most prescribed methods of cancer treatments but these are highly toxic and also are carcinogenic thus alternative cures are being explored especially on the use of cytotoxic substances isolated from natural sources like plants known for their ethnomedicinal properties. Natural chemopreventive agents such as antioxidants isolated from plants are argued to be important in suppressing or reversing carcinogenesis and to prevent the development of invasive cancers thus research studies are now currently geared in the evaluation and determination of the cytotoxicity and antioxidant activity of plant extracts to serve as ideal cure. Since the acquisition and processing of highly cytotoxic and selective effects of plant-based compounds to cancer cells are still limited, this study was conducted on eight plant species known to have folkloric medicinal properties. The leaf ethanolic extracts were examined for their antioxidant properties and cytotoxicity against Human Non-Small Cell Lung adenocarcinoma (A549), Human Colon Carcinoma Cells (HCT116) and Chinese Hamster Normal Ovary Cells (AA8). Antioxidant properties were observed to be high in seven species. Only one species P. edule to have low antioxidant property but high toxicity to HCT116 cancer cell lines. Three other species C. ovatum, F. nota and P. odorata were both having high antioxidant properties and cytotoxicity to cancer cell lines HCT116 while four species S. contorta, T. copelandii, C. ramiflora and P. arborea have high antioxidant but relative very low toxicity to HCT116 cancer cell lines. While two species namely, C. ovatum and P. edule were cytotoxic to HCT116 cancer cell lines, only C. ovatum was found to be cytotoxic for both A549 cancer cell lines thus could be a good candidate as source of chemotherapeutic and chemopreventive compounds. The results that showed seven out of the eight species have high percentage of antioxidants can be argued that these species are candidate species as sources of chemopreventive compounds. Further studies are still needed to determine the compounds from these species that confer selective cytotoxicity to cancer cell lines.

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Cytotoxic activity of crude extracts and fractions from Premna odorata (Blanco), Artocarpus camansi (Blanco) and Gliricidia sepium (Jacq.) against selected human cancer cell lines

Cited by 23 publications

References 17 publications

Cytotoxicity of selected Ethiopian medicinal plants used in traditional breast cancer treatment against breast-derived cell lines

Cytotoxicity of selected Ethiopian medicinal plants used in traditional breast cancer treatment against breast-derived cell lines

In vitro investigation of cytotoxic and antioxidative activities of Ardisia crispa against breast cancer cell lines, MCF-7 and MDA-MB-231

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