Cytotoxic activity of crude extracts of Dendrobium amoenum and detection of bioactive compounds by GC-MS

Paudel, Mukti Ram; Pant, Bijaya

doi:10.3126/botor.v11i0.21030

Cited by 13 publications

(7 citation statements)

References 22 publications

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“…The result clearly indicated that the extracts have some cytotoxic compounds, but other compounds are also present that may attribute to the cytotoxic capacity of the extracts as compared to the cisplatin. Previous reports enlightened that the methanol extract of some Dendrobium species showed cytotoxicity toward the HeLa and U251 cell lines [32,33,44]. Our results support the previous studies, which showed that polyphenol derivatives are responsible for the induction of apoptosis in cancer cells [45].…”

Section: Resultssupporting

confidence: 92%

Assessment of Antioxidant and Cytotoxic Activities of Extracts of Dendrobium crepidatum

et al. 2019

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Dendrobium crepidatum is an epiphytic orchid found in south Asia including Nepal and China. This orchid species is widely used in traditional Chinese medicine (TCM) for the treatment of cancer, diabetes, cataracts, and fever. The objectives of the present research were to assess the antioxidant and cytotoxic properties of its stem’s extracts with the identification of bioactive secondary metabolites. The antioxidant and cytotoxic activities were evaluated using the DPPH (2,2-diphenyl-1-picrylhydrazyl) and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays, respectively, and compounds were identified using GC–MS (gas chromatography and mass spectrometry). Ethanol and acetone extracts scavenged 94.69 ± 0.10% and 93.41 ± 0.86% of DPPH free radicals, respectively. They showed 50% inhibition of DPPH free radicals (IC50) at concentrations of 73.90 µg/mL and 99.44 µg/mL, which were found to be statistically similar to that of ascorbic acid (control). Chloroform extract inhibited the growth of 81.49 ± 0.43% of HeLa (human cervical carcinoma) cells and hexane extract inhibited the growth of 76.45 ± 4.26% of U251 (human glioblastoma) cells at 800 µg/mL concentration. These extracts showed 50% inhibition of cell growth (IC50) toward both the HeLa and U251 cell lines at their high concentrations, which were found statistically significantly different from that of cisplatin drug (control). The above extracts showed antioxidant and cytotoxic properties, potentially due to the presence of tetracosane, triacontane, stigmasterol, and some phenol derivatives (2-methoxy-4-vinylphenol, 2-methoxy-5-(1-propenyl)-phenol, p-mesyloxyphenol, and 2,6-dimethoxy-4-(2-propenyl)-phenol). This study explores the potential of this orchid in alternative medicine toward the development of drugs from its medicinally active compounds.

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Section: Resultssupporting

confidence: 92%

Assessment of Antioxidant and Cytotoxic Activities of Extracts of Dendrobium crepidatum

et al. 2019

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“…Additionally, studies have reported the presence of cholesterol 3-O[(2-acetoxy) ethyl] at a rate of 0.37% in Dendrobium amoenum. 48 However, our study shows that the presence of cholesterol at a rate of 13% in the plant is higher than that found in D. amoenum. On the other hand, literature surveys have shown that DL-glyceraldehyde is not detected in orchid plants.…”

Section: Discussioncontrasting

confidence: 73%

“…vomeracea. Previous studies have reported that Dendrobium amoenum contains 26.95% oleic acid, while Dendrobium aphyllum contains 8.6% oleic acid in its leaf methanol extract . Furthermore, Lusia tenuifolia was found to contain 1.9% oleic acid .…”

Section: Discussionmentioning

confidence: 84%

Cultivation of Serapias orientalis Plant Using Symbiotic Methods and Investigation of Bioactive Compounds

Aytar,

Kömpe

2024

ACS Agric. Sci. Technol.

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Conservation biology aims to prevent the decline in biodiversity by reducing the factors that cause it. One way to achieve this is by producing economically valuable orchids in high quantities, allowing humans to rely less on natural populations. In the study, Serapias orientalis seeds were successfully germinated using symbiotic methods and transplanted into a natural field. After 18 months, the plants began to flower, marking the first successful production of S. orientalis in a natural environment. The researchers then analyzed the fatty acid and chemical contents of the leaves and flowers using gas chromatography−mass spectrometry, identifying 28 fatty acids and 19 other substances. The most abundant fatty acids were linoleic acid (21.72%), palmitic acid (20.22%), allylinolenic acid (16.87%), oleic acid (8.67%), and stearic acid (6.13%). Major compounds identified in the methanol extract included hydroxyacetic acid hydrazide (7.38%), semicarbazide hydrochloride (5.06%), DL-glyceraldehyde (5.42%), pentacosane (39.85%), and cholesterol (13.05%). These findings offer a new method for conserving S. orientalis and highlight the potential use of these plants in food, medical, and industrial applications due to their high concentration of bioactive compounds.

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“…Their antibacterial, antifungal [ 28 ], insecticidal, antimicrobial [ 36 , 37 ], and preservative properties [ 27 ] have been reported. Six different types of fatty acid esters were identified in both methanol extracts with reported antioxidant, thyroid inhibitor [ 33 ], anticancer [ 34 ], anti-inflammatory [ 32 ], hemolytic, antiandrogenic, lubricant, pesticide, nematicide, hypocholesterolemic [ 30 ], and dienophilic [ 31 ] activities. Stigmasterol and β -sitosterol were only identified phytosterols with a wide range of pharmacological activities such as antimicrobial, anticancer, diuretic, anti-inflammatory, antioxidant, antiasthma, hepatoprotective, and anticancer activities [ 30 , 33 ].…”

Section: Resultsmentioning

confidence: 99%

Phytochemical, Cytotoxic, and Antimicrobial Evaluation of Tribulus terrestris L., Typha domingensis Pers., and Ricinus communis L.: Scientific Evidences for Folkloric Uses

Khalid

Algarni

Homeida

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Many medicinal plants have been utilized for centuries despite the lack of scientific evidence of their therapeutic effects. This study evaluated the phytochemical and dual biological profiling, namely, antibacterial and cytotoxic properties, of three plant species, namely, Tribulus terrestris L., Typha domingensis Pers., and Ricinus communis L., in order to explore potential relationships (if any) with their ethnopharmacological uses. GC-MS was used to achieve phytochemical screening of two plant extracts (T. terrestris and T. domingensis). The primary chemicals detected in varying amounts in both extracts were siloxane derivatives, fatty acid esters, diisooctyl phthalate, phytosterol, and aromatic acid esters. According to the findings, the major component detected in both extracts was 1,2-benzenedicarboxylic acid and diisooctyl ester (antibacterial and antifungal). T. domingensis contained a low level of benzoic acid, methyl ester (antibacterial). Both extracts included stigmasterol and sitosterol, as well as six different forms of fatty acid esters. Antimicrobial, antioxidant, anticancer, thyroid inhibitor, and anti-inflammatory properties have all been described. Human breast adenocarcinoma (MCF7), human ovary adenocarcinoma (A2780), and human colon adenocarcinoma (HT29), as well as normal human fetal lung fibroblasts (MRC5), all showed cytotoxic activity. The most potent activity against A2780 cells was seen in T. terrestris and T. domingensis extracts (IC50: 3.69 and 5.87 g/mL, respectively). R. communis was more active against MCF7 cells (1.52 μg/mL) followed by A2780 and HT29 cells, respectively. R. communis showed a dose-dependent clonogenic effect against MCF7 cells. The antibacterial activity of all three plant extracts was tested against three standard Gram-positive, four standard Gram-negative, and two clinical bacterial strains. Among the three extracts examined, T. terrestris was the most effective, followed by R. communis, and finally, T. domingensis plant extract was effective against various isolated bacteria. This study, interestingly, sheds light on the bioactive components found in plant extracts that can be utilized for cytotoxic and antibacterial purposes.

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Cytotoxic activity of crude extracts of Dendrobium amoenum and detection of bioactive compounds by GC-MS

Cited by 13 publications

References 22 publications

Assessment of Antioxidant and Cytotoxic Activities of Extracts of Dendrobium crepidatum

Assessment of Antioxidant and Cytotoxic Activities of Extracts of Dendrobium crepidatum

Cultivation of Serapias orientalis Plant Using Symbiotic Methods and Investigation of Bioactive Compounds

Phytochemical, Cytotoxic, and Antimicrobial Evaluation of Tribulus terrestris L., Typha domingensis Pers., and Ricinus communis L.: Scientific Evidences for Folkloric Uses

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