Cytotoxic Activity of Paris quadrifolia Extract and Isolated Saponin Fractions Against Human Tumor Cell Lines

Stefanowicz–Hajduk, Justyna; Kawiak, Anna; Gajdus, Jerzy; Ochocka, J. Renata; Paszkiewicz, Monika; Stepnowski, Piotr; Łojkowska, Ewa

doi:10.2478/v10182-011-0024-1

Cited by 2 publications

(3 citation statements)

References 19 publications

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“…It has been reported that saponins possess potent cytotoxic activity. [37] The general lack of cytotoxicity observed in this study is confirmed by literature. Apu et al [35] reported negligible in-vitro cytotoxicity of the n-hexane, ethyl acetate and methanol extracts of the aerial parts of the plant.…”

Section: Discussionsupporting

confidence: 89%

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Attenuation of oxidative stress and artificial wound closure in C2C12 myoblasts induced by sequential extracts of Boerhavia diffusa

Yahaya

Cordier

Steenkamp

et al. 2018

Journal of Pharmacy and Pharmacology

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Objectives Whole plants of Boerhavia diffusa L. are widely used medicine in Ghana and other tropical countries, for the treatment of wounds and other ailments. The aim of the study was to determine the ability of sequential extracts of B. diffusa to influence oxidation and wound closure in myoblast cells in vitro.Methods Sequential extracts were prepared from the whole plant using four solvents of increasing polarity (hexane, ethyl acetate, methanol and water). Cytotoxicity was determined using the sulforhodamine B staining assay, phase-contrast microscopy, plasDIC microscopy and live-dead staining. Extracts were tested for their ability to reduce 2,2 0 -azobis(2-amidinopropane) dihydrochloride (AAPH)-induced oxidation and mediate cell migration after artificial wound generation in C2C12 myoblast cells using the scratch wound assay. Key findings All extracts indicated negligible cytotoxicity (IC 50 > 100 lg/ml), and microscopic evaluation showed no difference from negative controls. AAPH induced a 2.87-fold increase in reactive oxygen species compared to the negative control. Pretreatment with 100 lg/ml of the extracts reduced AAPH-induced oxidation to 1.70-fold of the untreated controls (P < 0.001). Wound closures in the methanol and water extract treatments were 18.08% and 20.76% higher than the negative control, respectively (P < 0.01). Conclusions These findings indicate that the hexane, methanol and water extracts of B. diffusa whole plant promote artificial wound healing and protection against oxidation in vitro and therefore warrant further research into its mechanisms of wound healing.

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Section: Discussionsupporting

confidence: 89%

“…In contrast to treatment with extracts, treatment with saponin resulted in significant cellular damage and death. It has been reported that saponins possess potent cytotoxic activity . The general lack of cytotoxicity observed in this study is confirmed by literature.…”

Section: Discussionmentioning

confidence: 99%

Attenuation of oxidative stress and artificial wound closure in C2C12 myoblasts induced by sequential extracts of Boerhavia diffusa

Yahaya

Cordier

Steenkamp

et al. 2018

Journal of Pharmacy and Pharmacology

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“…[ 1 ] The plant occurs in deciduous forests in Poland and studies of this species are conducted at present. [ 2 3 ]…”

Section: Introductionmentioning

confidence: 99%

Isolation and identification of cytotoxic compounds from the rhizomes of Paris quadrifolia L.

et al. 2014

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Background:Paris quadrifolia L. is a medicinal plant which contains steroidal saponins. The present study reports isolation and structural identification of six pennogenyl saponins obtained from P. quadrifolia rhizomes. The four spirostan saponins were obtained from P. quadrifolia for the first time. The cytotoxic effects of the sub-fractions and six compounds isolated from the plant extract were evaluated on tumour cells.Materials and Methods:Ethanol extract from the rhizomes of P. quadrifolia were partinioned using column chromatography. The saponins were isolated from the obtained sub-fractions by isocratic RP HPLC and their structures were determined by means of 1D and 2D NMR spectroscopy and MALDI TOF MS. The cytotoxic effects of the sub-fractions and the isolated compounds were tested against human promyelocytic leukaemia cells (HL-60), human cervical adenocarcinoma cells (HeLa) and human breast cancer cells (MCF-7) using the [(3-(4,5-dimethylthiazol-2-yl)]-2,5-diphenyltetrazolium bromide (MTT) assay.Results:Six pennogenyl saponins were isolated from P. quadrifolia rhizomes: pennogenin 3-O-β-D-glucopyranoside (1), pennogenin 3-O-α-L-rhamnopyranosyl-(1→4)-β-D-glucopyranoside (2), pennogenin 3-O-α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranoside (3), pennogenin 3-O-α-L-rhamnopyranosyl-(1→4)-α-L-rhamnopyranosyl-(1→4)-β-D-glucopyranoside (4), pennogenin 3-O-α-L-rhamnopyranosyl-(1→4)-[α-L-rhamnopyranosyl-(1→2)]-β-D-glucopyranoside (5), pennogenin 3-O-α-L-rhamnopyranosyl-(1→4)-α-L-rhamnopyranosyl-(1→4)-[α-L-rhamnopyranosyl-(1→2)]-β-D-glucopyranoside (6). Pennogenyl saponins 5 and 6 exhibited cytotoxic activity against HL-60, HeLa and MCF-7 tumour cells with IC50 values of 1.0 ± 0.04 μg/ml, 1.8 ± 0.072 μg/ml and 2.4 ± 0.096 μg/ml respectively, and 2.0 ± 0.08 μg/ml, 2.5 ± 0.125 μg/ml and 3.2 ± 0.128 μg/ml respectively.Conclusion:Compounds 1-4 were isolated from this species for the first time.

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Cytotoxic Activity of Paris quadrifolia Extract and Isolated Saponin Fractions Against Human Tumor Cell Lines

Cited by 2 publications

References 19 publications

Attenuation of oxidative stress and artificial wound closure in C2C12 myoblasts induced by sequential extracts of Boerhavia diffusa

Attenuation of oxidative stress and artificial wound closure in C2C12 myoblasts induced by sequential extracts of Boerhavia diffusa

Isolation and identification of cytotoxic compounds from the rhizomes of Paris quadrifolia L.

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