Cytotoxic Activity of Zinc Oxide Nanoparticle Synthesis Using Leaves Extract of Abies webbiana

Srinisha, M; Jeevitha, M.; Rajeshkumar, S.; Jayaraman, Selvaraj

doi:10.9734/jpri/2021/v33i60b35067

Cited by 2 publications

(2 citation statements)

References 19 publications

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“…Although there were no fatalities observed at greater dosages, this underscores that the cytotoxic effect is a dose-dependent effect [ 18 ]. Moreover, green-synthesised ZnONPs using C oriander oleoresin demonstrated promising outcomes in cytotoxicity assessments, showcasing reduced toxicity levels [ 19 ]. In addition, findings indicated that ZnONPs produced from Abies webbiana show less toxic effects comparatively.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Antimicrobial and Cytotoxic Activity of Nanoformulated Chamomile and Green Tea-Based Mouthwash: An In Vitro Study

Chatterjee,

Ramamurthy

2024

Cureus

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Introduction Nanotechnology plays a significant role in the biomedical and dental fields, offering numerous benefits to humans. Particularly, nanoparticles synthesised through green methods involving herbal formulations present promising advantages. Zinc oxide nanoparticles (ZnONPs) demonstrate strong antibacterial properties. Utilising treatments incorporating chamomile tea and green tea may potentially reduce toxicity while enhancing antibacterial effectiveness against oral infections. This study aimed to develop a mouthwash containing ZnONPs, followed by an evaluation of both its cytotoxicity and antibacterial effectiveness. Materials and methods This study was conducted at Saveetha Dental College, Saveetha Institute of Medical and Technical Science, Chennai, India. In the synthesis of ZnONPs, a formulation consisting of chamomile tea and green tea was employed. Subsequently, these synthesised nanoparticles were used in the preparation of mouthwash. An antimicrobial test of the produced ZnONPs was carried out using the agar well diffusion technique for oral pathogens. For analysis of cytotoxicity, brine shrimps were used in an assay, and comparisons were made with a commercially available mouthwash. Results The antimicrobial properties were assessed, and the formulated mouthwash demonstrated a zone of inhibition of Staphylococcus aureus (20 mm) , Enterococcus faecalis (11 mm) , Streptococcus mutans (15 mm) and Candida albicans (13 mm), when the agar well diffusion assay was carried out. Furthermore, the formulated mouthwash exhibited lower cytotoxicity compared to the commercially available mouthwash when cytotoxicity was checked in brine shrimps. Conclusion In our study, the ZnONP synthesis with chamomile tea and green tea showed notable antibacterial and antifungal effects. In addition, lower toxicity was observed compared to the commercially available mouthwash. These findings suggest that mouthwash formulated with green-synthesis ZnONPs could serve as a viable alternative to synthetic mouthwash options. As a result, it is suggested that ZnONPs could be employed in mouthwash formulations at concentrations of 40 µL.

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Section: Discussionmentioning

confidence: 99%

Evaluation of Antimicrobial and Cytotoxic Activity of Nanoformulated Chamomile and Green Tea-Based Mouthwash: An In Vitro Study

Chatterjee,

Ramamurthy

2024

Cureus

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“…While there is no fatality rate at higher dosages, this demonstrates that the cytotoxic impact is dose-dependent [ 20 ]. Furthermore, the green-synthesized ZnONPs derived from coriander oleoresin showed encouraging results in cytotoxicity tests as they exhibited lower toxicity [ 21 ]. The results also revealed that ZnONPs synthesized from Abies webbiana are less toxic.…”

Section: Discussionmentioning

confidence: 99%

Preparation of a Herbal Mouthwash With Lemongrass and Mint-Mediated Zinc Oxide Nanoparticles and Evaluation of Its Antimicrobial and Cytotoxic Properties

Shanmugam,

Govindharaj,

Arunkumar

et al. 2024

Cureus

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Introduction Nanotechnology holds considerable importance in biomedical and dental applications. Nanoparticles synthesized using green synthesis methods with herbal formulations offer various benefits to humans. Zinc oxide nanoparticles (ZnONPs), being semiconductors, exhibit potent antibacterial properties. Notably, treatments utilizing lemongrass and mint ensure potentially lower toxicity and antibacterial qualities for oral infections. The goal of the study is to prepare a mouthwash mediated by ZnONPs and assess its cytotoxic potential and antibacterial efficacy. Materials and methods A lemongrass and mint formulation was used in the synthesis of ZnONPs, and the mouthwash was prepared using the synthesized nanoparticles. The produced ZnONPs were tested for their antimicrobial activity using agar well diffusion technique against oral pathogens, and the ZnONPs-mediated mouthwash was evaluated for its cytotoxic effect using the brine shrimp lethality assay and compared to commercial mouthwash. Results The green-synthesized ZnONPs were initially confirmed using a UV-visible spectrophotometer and exhibited a maximum peak at 362 nm. The antimicrobial activity was tested for the synthesized ZnONPs against oral pathogens, which showed a maximum zone of inhibition of 22 mm in Enterococcus faecalis and 23 mm in Candida albicans , as estimated by the agar well diffusion technique. Additionally, ZnONPs-based herbal mouthwash demonstrated lower cytotoxicity than the commercial mouthwash in the brine shrimp lethality assay. Conclusion In the current study, lemongrass and mint-mediated ZnONPs demonstrated an effective antibacterial activity against E. faecalis and antifungal activity against C. albicans . Furthermore, the cytotoxic effect tested using the brine shrimp lethality assay for ZnONPs-mediated mouthwash demonstrated lower toxicity as compared to the commercial mouthwash. This suggests that the green-synthesized ZnONPs-based mouthwash could be used as an alternative to synthetic mouthwash.

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Cytotoxic Activity of Zinc Oxide Nanoparticle Synthesis Using Leaves Extract of Abies webbiana

Cited by 2 publications

References 19 publications

Evaluation of Antimicrobial and Cytotoxic Activity of Nanoformulated Chamomile and Green Tea-Based Mouthwash: An In Vitro Study

Evaluation of Antimicrobial and Cytotoxic Activity of Nanoformulated Chamomile and Green Tea-Based Mouthwash: An In Vitro Study

Preparation of a Herbal Mouthwash With Lemongrass and Mint-Mediated Zinc Oxide Nanoparticles and Evaluation of Its Antimicrobial and Cytotoxic Properties

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