Cytotoxic and Apoptosis-Inducing Activities of 12-&lt;i&gt;O&lt;/i&gt;-Acetylazedarachin B from the Fruits of &lt;i&gt;Melia azedarach&lt;/i&gt; in Human Cancer Cell Lines

Kikuchi, Takashi; Pan, Xin; Ishii, Koichi; Nakamura, Yasuhiro; Ogihara, Eri; Koike, Kazuo; Tanaka, Reiko; Akihisa, Toshihiro

doi:10.1248/bpb.b12-00795

Cited by 20 publications

(14 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among the caspases identified in mammalian cells, caspase-3, -7, and -9 may serve as effector caspases of apoptotic cell death (26,35,36). Caspase-3, -7, and -9 were synthesized as inactive proenzymes (sizes 32, 35, and 47 kDa, respectively), which require proteolytic activation to cleaved enzymes (sizes 17, 20, and 37 kDa, respectively) (26,35,36). In this study, the immunoblotting assays for the expression of the apoptotic factors (PARP and cleaved caspase-3, -7, and -9) were performed to confirm the JPH203-induced apoptosis in YD-38 cells.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

JPH203, an L-Type Amino Acid Transporter 1–Selective Compound, Induces Apoptosis of YD-38 Human Oral Cancer Cells

et al. 2014

View full text Add to dashboard Cite

Abstract. Compared to most normal cells that express L-type amino acid transporter 2, L-type amino acid transporter 1 is highly expressed in cancer cells and presumed to support their elevated growth and proliferation. This study examined JPH203, a potent and selective L-type amino acid transporter 1 inhibitor, and its ability to suppress YD-38 human oral cancer cell growth. The YD-38 cells express L-type amino acid transporter 1 with its associating protein 4F2 heavy chain, but not L-type amino acid transporter 2. JPH203 and BCH, a non-selective L-type amino acid transporter inhibitor, completely inhibited l-leucine uptake in YD-38 cells. As expected, the intrinsic affinity of JPH203 to inhibit l-leucine uptake was far more efficient than BCH. Likewise, JPH203 and BCH inhibited YD-38 cell growth, with JPH203 being superior to BCH. JPH203 up-regulated the population of apoptotic YD-38 cells through the activation of apoptotic factors, including caspases and PARP. These results suggest that the inhibition of L-type amino acid transporter 1 activity via JPH203, which may act as a potential novel anti-oral-cancer agent, leads to apoptosis by inducing the intracellular depletion of the neutral amino acids essential for cancer cell growth in YD-38 human oral cancer cells.

show abstract

Section: Discussionmentioning

confidence: 99%

“…To determine if JPH203-induced cell death is associated with the induction of apoptosis, the YD-38 cells were stimulated with 3.0 mM JPH203 for 24 h and then co-stained with Annexin V-FITC, an apoptotic marker, and PI, a necrotic marker (26). As shown in Fig.…”

Section: Apoptosis Induction Of Yd-38 Cells By Jph203mentioning

confidence: 99%

JPH203, an L-Type Amino Acid Transporter 1–Selective Compound, Induces Apoptosis of YD-38 Human Oral Cancer Cells

et al. 2014

View full text Add to dashboard Cite

show abstract

“…A limonoid isolated from MA has been reported to possess anti-feeding and insecticidal activities (30). Moreover, several other constituents from MA possess anti-herpetic (31), anti-angiogenic (32) and anticancer (33) properties. However, despite previous findings, the effect of MA on melanogenesis has not been reported.…”

Section: Discussionmentioning

confidence: 99%

Melia azedarach extract stimulates melanogenesis through increase of tyrosinase-related protein 1 expression in B16F10 mouse melanoma cells

Cheng

Jin

et al. 2015

International Journal of Molecular Medicine

View full text Add to dashboard Cite

Abstract. Melia azedarach (MA) has been used in folk medicine in Asia for the treatment of several diseases. Several constituents from MA possess anti-herpetic, anti-angiogenic and anticancer properties. The aim of the present study was to investigate the effect of a 70% ethanol extract of MA on melanogenesis and the underlying mechanisms involved. A B16F10 mouse melanoma cell line was used in our experiments. Treatment of B16F10 cells with the MA extract (10, 20 and 40 µg/ml) increased melanin content in a concentration-dependent manner without cytotoxicity at 24 h. Further experiments indicated that the MA extract (20 µg/ml) increased melanin content as early as at 4 h after treatment. Additionally, although the MA extract did not affect intracellular tyrosinase activity and the protein levels of tyrosinase and tyrosinase-related protein-2 (TRP-2) at 2 and 4 h after treatment, the MA extract increased TRP-1 protein expression at both time points. However, no significant effect of the MA extract treatment on TRP-1 mRNA level at the time points measured was observed. In conclusion, the results from the present study demonstrate that the MA extract increases melanogenesis through the upregulation of TRP-1 protein expression by post-transcriptional control in B16F10 cells and suggest that the MA extract can be viewed as a rapid inducer of melanogenesis, thus rendering it a potential treatment for hypopigmentation diseases including vitiligo.

show abstract

“…The limonoids of M . azedarach have been reported to exhibit cytotoxic, antimicrobial, antioxidant, antifeedant, insecticidal, and antiviral activities. In the course of a search for potential bioactive compounds from Meliaceae plants, we investigated the constituents of M .…”

Section: Introductionmentioning

confidence: 99%

Four New Tirucallane Triterpenoids from the Fruits of Melia azedarach and Their Cytotoxic Activities

Zhou

et al. 2016

Chemistry & Biodiversity

View full text Add to dashboard Cite

Four new tirucallane triterpenoids, (21S,23R,24R)-21,23-epoxy-21,24-dihydroxy-25-methoxytirucall-7-en-3-one (2), (3S,21S,23R,24S)-21,23-epoxy-21,25-dimethoxytirucall-7-ene-3,24-diol (8), (21S,23R,24R)-21,23-epoxy-24-hydroxy-21-methoxytirucalla-7,25-dien-3-one (11), and (21S,23R,24R)-21,23-epoxy-21,24-dihydroxytirucalla-7,25-dien-3-one (12), along with 16 known analogues, 1, 3 - 7, 9 - 10, and 13 - 20, were isolated from the fruits of Melia azedarach. Their structures were elucidated by spectroscopic methods including 1D- and 2D-NMR techniques and mass spectrometry. These compounds were evaluated for their cytotoxicities against HepG2 (liver), SGC7901 (stomach), K562 (leukemia), and HL60 (leukemia) cancer cell lines. Compound 20 exhibited potent cytotoxicity against HepG2 and SGC7901 cancer cells with the IC values of 6.9 and 6.9 μm, respectively.

show abstract

Cytotoxic and Apoptosis-Inducing Activities of 12-<i>O</i>-Acetylazedarachin B from the Fruits of <i>Melia azedarach</i> in Human Cancer Cell Lines

Cited by 20 publications

References 27 publications

JPH203, an L-Type Amino Acid Transporter 1–Selective Compound, Induces Apoptosis of YD-38 Human Oral Cancer Cells

JPH203, an L-Type Amino Acid Transporter 1–Selective Compound, Induces Apoptosis of YD-38 Human Oral Cancer Cells

Melia azedarach extract stimulates melanogenesis through increase of tyrosinase-related protein 1 expression in B16F10 mouse melanoma cells

Four New Tirucallane Triterpenoids from the Fruits of Melia azedarach and Their Cytotoxic Activities

Contact Info

Product

Resources

About