Cytotoxic and apoptosis-inducing properties of a C21-steroidal glycoside isolated from the roots of Cynanchum auriculatum

Ye, Liang‐Fei; Wang, Yiqi; Yang, Bo; Zhang, Rusong

doi:10.3892/ol.2013.1186

Cited by 9 publications

(4 citation statements)

References 15 publications

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“…Current studies have explained the anticancer activity of C. auriculatum extracts and the potential mechanisms involved [45,46]. However, caudatin, a C-21 steroid derived from C. auriculatum, has not been investigated in selective cytotoxicity against human CSCs.…”

Section: Discussionmentioning

confidence: 99%

Caudatin Isolated from Cynanchum auriculatum Inhibits Breast Cancer Stem Cell Formation via a GR/YAP Signaling

et al. 2020

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In the complex tumor microenvironment, cancer stem cells (CSCs), a rare population of cells, are responsible for malignant tumor initiation, metastasis, drug resistance and recurrence. Controlling breast CSCs (BCSCs) using natural compounds is a novel potential therapeutic strategy for clinical cancer treatment. In this study, a mammosphere assay-guided isolation protocol including silica gel, a C18 column, gel filtration, and high-pressure liquid chromatography was used to isolate an inhibitory compound from Cynanchum auriculatum extracts. The isolated inhibitory compound was identified as caudatin. Caudatin inhibited breast cancer cell proliferation, mammosphere formation and tumor growth. Caudatin decreased the CD44+/CD24− and aldehyde dehydrogenase+ cell proportions and the levels of c-Myc, Oct4, Sox2, and CD44. Caudatin induced ubiquitin (Ub)-dependent glucocorticoid receptor (GR) degradation and blocked subsequent Yes-associated protein (YAP) nuclear accumulation and target gene transcription signals in BCSCs. These results show that the GR/YAP signaling pathway regulates BCSC formation and that caudatin may be a potential chemopreventive agent that targets breast cancer cells and CSCs.

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Section: Discussionmentioning

confidence: 99%

Caudatin Isolated from Cynanchum auriculatum Inhibits Breast Cancer Stem Cell Formation via a GR/YAP Signaling

et al. 2020

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“…Apart from inhibition of cell proliferation, apoptosis is another important event that anticancer drugs target [ 19 ]. In the previous study, we found different C 21 -steroidal glycosides with different cytotoxicity and apoptosis inducing properties [ 20 , 21 ]. Therefore, we also explored whether the growth inhibition effect of CGII is related to apoptosis.…”

Section: Discussionmentioning

confidence: 99%

A C₂₁-Steroidal Glycoside Isolated from the Roots ofCynanchum auriculatumInduces Cell Cycle Arrest and Apoptosis in Human Gastric Cancer SGC-7901 Cells

Wang

Zhang

Hong

et al. 2013

Evidence-Based Complementary and Alternative Medicine

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Caudatin 3-O-β-D-cymaropyranosyl-(1 → 4)-β-D-oleandropyranosyl-(1 → 4)-β-D-cymaropyranosyl-(1 → 4)-β-D-cymaropyranoside (CGII) is one of the C21-steroidal glycosides isolated from the roots of Cynanchum auriculatum ROYLE ex WIGHT. This study aimed to determine the cell growth, cell proliferation, and apoptotic cell death of human gastric cancer cells after CGII treatment. MTT assay was used to determine cell growth; fluorescence-activated cell sorting analysis was used to evaluate cell cycle distribution and apoptotic cell death. Immunoblotting was applied for measuring the expression of proteins involved in the cell cycle progression. The activities of caspase-3, -8, and -9 were detected by colorimetric caspase activity assays. CGII inhibited cell growth of human gastric cancer SGC-7901 cells in a concentration- and time-dependent manner. Treatment of SGC-7901 cells with CGII resulted in G1 phase cell cycle arrest, accompanied with decreased expression of cyclin D1 and cyclin-dependent kinases 4 and 6. CGII induced cell apoptosis and activated caspase-3, caspase-8, and caspase-9. In contrast, pan-caspase inhibitor z-VAD-fmk partially abolished the CGII-induced growth inhibition of SGC-7901 cells. In conclusion, CGII inhibits cell growth of human gastric cancer cells by inducing G1 phase cell cycle arrest and caspase-dependent apoptosis cascades.

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“…Similarly, the same and novel C21-steroidal glycosides were also detected in C. auriculatum and C. wilfordii (Li et al, 2008;Lu, Xiong, Hong, Guang, & Zhi, 2011;Xiang, Ma, & Hu, 2010;Ye, Wang, Yang, & Zhang, 2013;Zhang, Ye, Shen, & Liang, 2000). Besides, novel compounds, such as Wilfoside K1N inhibiting angiogenesis (Kim et al, 2005), cynandione A protecting from cytotoxicity (Lee et al, 2015), and amino-substituted p-benzoquinone (Yeo & Kim, 1997), were isolated from roots of C. wilfordii.…”

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confidence: 87%

Selection of solvent for extraction of antioxidant components from Cynanchum auriculatum, Cynanchum bungei, and Cynanchum wilfordii roots

Wu¹,

Zhang²,

He³

et al. 2019

Food Science & Nutrition

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In east Asia, “Baishouwu” has been used as an herbal drug and functional dietary supplement for hundreds of years. Actually, “Baishouwu” is the common name of the roots of Cynanchum auriculatum , Cynanchum bungei , and Cynanchum wilfordii . In the present study, roots of these three specie were extracted and then fractionated using petroleum ether (PE), dichloromethane (DCM), ethyl acetate (EA), and water. DPPH scavenging experiments revealed high antioxidant activity of DCM and EA fractions of C. bungei and the EA fraction of C. wilfordii . Treatments with these three fractions significantly reduced malondialdehyde content in heat‐stressed Daphnia magna , validating in vivo antioxidant activity. Gas chromatography–mass spectrometer (GC‐MS) analyses demonstrated that the chemical components of fractions extracted from C. bungei , C. bungei , and C. wilfordii were different. Further determination of total phenol and total flavonoids contents showed that DCM and EA fractions of C. bungei and EA fraction of C. wilfordii had much higher contents of total phenol and total flavonoids, which might be the reason to explain their strong antioxidant activity. Overall, the present study suggested that these three plants have different chemical components and biological activities. They could not be used as the same drug.

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Cytotoxic and apoptosis-inducing properties of a C21-steroidal glycoside isolated from the roots of Cynanchum auriculatum

Cited by 9 publications

References 15 publications

Caudatin Isolated from Cynanchum auriculatum Inhibits Breast Cancer Stem Cell Formation via a GR/YAP Signaling

Caudatin Isolated from Cynanchum auriculatum Inhibits Breast Cancer Stem Cell Formation via a GR/YAP Signaling

A C₂₁-Steroidal Glycoside Isolated from the Roots ofCynanchum auriculatumInduces Cell Cycle Arrest and Apoptosis in Human Gastric Cancer SGC-7901 Cells

Selection of solvent for extraction of antioxidant components from Cynanchum auriculatum, Cynanchum bungei, and Cynanchum wilfordii roots

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Cytotoxic and apoptosis-inducing properties of a C21-steroidal glycoside isolated from the roots of Cynanchum auriculatum

Cited by 9 publications

References 15 publications

Caudatin Isolated from Cynanchum auriculatum Inhibits Breast Cancer Stem Cell Formation via a GR/YAP Signaling

Caudatin Isolated from Cynanchum auriculatum Inhibits Breast Cancer Stem Cell Formation via a GR/YAP Signaling

A C21-Steroidal Glycoside Isolated from the Roots ofCynanchum auriculatumInduces Cell Cycle Arrest and Apoptosis in Human Gastric Cancer SGC-7901 Cells

Selection of solvent for extraction of antioxidant components from Cynanchum auriculatum, Cynanchum bungei, and Cynanchum wilfordii roots

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A C₂₁-Steroidal Glycoside Isolated from the Roots ofCynanchum auriculatumInduces Cell Cycle Arrest and Apoptosis in Human Gastric Cancer SGC-7901 Cells