Cytotoxic and genotoxic characterization of aluminum and silicon oxide nanoparticles in macrophages

Hashimoto, Masanori; Imazato, Satoshi

doi:10.1016/j.dental.2015.02.009

Cited by 38 publications

(37 citation statements)

References 29 publications

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“…In vitro, Al 2 O 3 NMs were found to induce MN in a concentration dependent manner after 24 h exposure from 0.5 µg/mL in Chinese hamster cells CHOK1 [19] and in human fibroblasts at 13.3 and 26.6 µg/cm 2 (50 and 100 µg/mL) [16]. In contrast, no MN increase was found in human blood lymphocytes [34] or RAW 264 macrophages exposed for 72 h to Al 2 O 3 NMs [35]. A negative response was also observed with the chromosome aberrations assay in human lymphocytes with Al 2 O 3 NMs [18].…”

Section: Discussionmentioning

confidence: 91%

Genotoxicity of Aluminum and Aluminum Oxide Nanomaterials in Rats Following Oral Exposure

et al. 2020

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Due to several gaps remaining in the toxicological evaluation of nanomaterials (NMs), consumers and public health agencies have shown increasing concern for human health protection. In addition to aluminum (Al) microparticles, Al-containing nanomaterials (Al NMs) have been applied by food industry as additives and contact materials. Due to the limited amount of literature on the toxicity of Al NMs, this study aimed to evaluate the in vivo genotoxic potential of Al 0 and Al 2 O 3 NMs after acute oral exposure. Male Sprague-Dawley rats were administered three successive gavages at 6, 12.5 and 25 mg/kg bw. A comparison with AlCl 3 was done in order to assess the potential effect of dissolution into Al ions. Both DNA strand breaks and oxidative DNA damage were investigated in six organs/tissues (duodenum, liver, kidney, spleen, blood and bone marrow) with the alkaline and the Fpg-modified comet assays. Concomitantly, chromosomal damage was investigated in bone marrow and colon with the micronucleus assay. The comet assay only showed DNA damage with Al 2 O 3 NMs in bone marrow (BM), while AlCl 3 induced slight but non-significant oxidative DNA damage in blood. No increase of chromosomal mutations was observed after treatment with the two Al MNs either in the BM or in the colons of rats.

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Section: Discussionmentioning

confidence: 91%

Genotoxicity of Aluminum and Aluminum Oxide Nanomaterials in Rats Following Oral Exposure

et al. 2020

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“…Nevertheless, the increase of DNA fragmentation in these latter studies was probably linked to cell death detected by Trypan blue exclusion [56] or by apoptotic markers [22,24].…”

Section: Discussionmentioning

confidence: 73%

“…Additionally, Al 2 O 3 NMs did not induce DNA damage in the alkaline comet assay in Caco-2 and HepaRG cells following a 24 h treatment. Although some studies have reported negative results in the comet assay in human lymphocytes and in human embryonic kidney cells [20,26], others have demonstrated time-and/or concentration-dependent genotoxic effects in Chinese hamster lung broblasts [56], in RAW264 murine macrophages [24] and in human liver HepG2 cells [22] treated with Al 2 O 3 NMs.…”

Section: Discussionmentioning

confidence: 99%

“…Chinese hamster ovary cells [23]. While some studies have not observed genotoxic effects of Al 2 O 3 NMs [20,24,25,26], others have reported a positive response [21,22] which may be associated with oxidative damage [22]. In contrast, no data on the in vitro genotoxicity of Al 0 NMs has been published so far, and only some cytotoxicity was detected in rat alveolar macrophages treated with Al 0 above 100 µg/ml [27].…”

Section: Introductionmentioning

confidence: 99%

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Genotoxic Impact of Aluminum-containing Nanomaterials in Human Intestinal and Hepatic Cells.

Jalili

Huet

Burel³

et al. 2020

Preprint

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Background: Exposure of consumers to aluminum-containing nanomaterials (Al NMs) through numerous products is an area of concern for public health agencies since human health risks are not completely elucidated. In addition, the available data on the genotoxicity of Al2O3 and Al0 NMs are inconclusive or rare. In order to provide further information, the present study investigated the in vitro genotoxic potential of Al0 and Al2O3 NMs in intestinal and liver cell models since these tissues represent organs which would be in direct contact or could experience potential accumulation following oral exposure. Methods: Differentiated human intestinal Caco-2 and hepatic HepaRG cells were exposed to Al0 and Al2O3 NMs (0.03 to 80 µg/cm2) and the results were compared with those obtained with the ionic form AlCl3. Several methods, including H2AX labelling, the alkaline comet assay and micronucleus (MN) assays were used. Oxidative stress and oxidative DNA damage were assessed using High Content Analysis (HCA) and the formamidopyrimidine DNA-glycosylase -modified comet assay respectively. Moreover, carcinogenic properties of Al NMs were investigated through the cell transforming assay (CTA) in Bhas 42 cells.Results: The three forms of Al did not induce chromosomal damage when tested in the MN assay. Furthermore, no cell transformation was observed in Bhas 42 cells. However, although no production of oxidative stress was detected in HCA assays, Al2O3 NMs induced oxidative DNA damage in Caco-2 cells in the comet assay following a 24 h treatment. Considerable DNA damage was observed with Al0 NMs in both cell lines in the comet assay, although this was likely due to interference with these NMs. Finally, no genotoxic effects were observed with AlCl3. Conclusion: The slight effects observed with Al NMs are therefore not likely to be related to ion release in the cell media.

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“…Moreover, the small size of the NPs helps them to evade clearance mechanisms in the body; therefore, the retention time is further increased, rendering them more biopersistent [7]. These factors contribute to a increased potential for reactivity, resulting in higher ROS production, lipid peroxidation, and the damage to cell membranes [38][39][40]. This can ultimately lead to apoptotic cell death.…”

Section: Nanotoxicology Of Silicamentioning

confidence: 99%

Tools for Assessment of Occupational Health Risks of some Engineered Nanoparticles and Carbon Materials Used in Semiconductor Applications

Watjanatepin¹,

Prodanov²

2018

Occupational Health and Safety - A Multi-Regional Perspective

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Engineered nanomaterials (ENM) are used in a wide variety of applications: from cosmetics and paints to sportswear and semiconductor chips. While for chemicals there are established regulatory frameworks dealing with the risk for the consumers, workers, and the environment, this is not the case for nanomaterials. This is precisely why ENMs are used-the properties of matter change at the nanoscale and become dependent on the particle morphology and size. Our understanding on how such nano-systems react with biological matter, such as cells and tissues, is far from complete, and this brings about an increasing level of uncertainty in the research and development process. This chapter will give an overview of several materials, which are either used or have potential applications in nanoelectronics. While silicon dioxide and metal oxide nanoparticles are used in semiconductor processing in standard polishing steps, applications of carbon materials may be more disruptive. As promising materials with broad applications, we focus on carbon nanotubes and graphene. So-identified materials are used to illustrate the use of different risk assessment tools in the occupational setting of nanoelectronics. The application of such tools in itself is also a growing area of research efforts supported by international stakeholders, such as the European Commission.

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Cytotoxic and genotoxic characterization of aluminum and silicon oxide nanoparticles in macrophages

Cited by 38 publications

References 29 publications

Genotoxicity of Aluminum and Aluminum Oxide Nanomaterials in Rats Following Oral Exposure

Genotoxicity of Aluminum and Aluminum Oxide Nanomaterials in Rats Following Oral Exposure

Genotoxic Impact of Aluminum-containing Nanomaterials in Human Intestinal and Hepatic Cells.

Tools for Assessment of Occupational Health Risks of some Engineered Nanoparticles and Carbon Materials Used in Semiconductor Applications

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