Cytotoxic and genotoxic effects of resin monomers in human salivary gland tissue and lymphocytes as assessed by the single cell microgel electrophoresis (Comet) assay

Kleinsasser, Norbert; Schmid, Katharina; Sassen, A.; Harréus, Ulrich; Staudenmaier, R.; Folwaczny, Matthias; Glas, Jürgen; Reichl, Franz‐Xaver

doi:10.1016/j.biomaterials.2005.09.023

Cited by 135 publications

(111 citation statements)

References 36 publications

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“…4,5 and 6). In previous studies, it has been shown that Bis-GMA, HEMA and UDMA triggered DNA damage in human lymphocytes and human salivary gland tissue cells 34,35) . Similar to these findings, DNA single strand breaks (SSB) were significantly elevated following exposure to dentin bonding agents including dimethacrylate monomers, UDMA, HEMA and Bis-GMA in our study model supporting the genotoxic effects of these agents.…”

Section: Discussionmentioning

confidence: 99%

The protective effect of resveratrol against dentin bonding agents-induced cytotoxicity

Atalayın

Armağan

Konyalιoğlu

et al. 2015

Dental Materials Journal

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Bond were added to the medium using extract method. The cells were cultured with or without resveratrol (RES) addition. MTT, reactive oxygen species (ROS), DCF, Comet and 8-OHdG measurements were performed. The agents had a dose-dependent (1:1>1:10>1:20) cytotoxic effect. Considering 1:10 concentration; Group D at 1 h (p<0.01) and Group B and D at 24 h had the weakest cytotoxic effect (p<0.05). After RES addition, the highest cell viability was determined in Groups B+RES and D+RES at 1 h and in Groups A+RES and B+RES at 24 h (p<0.01). The dentin bonding agents induced ROS production and DNA damage regarding to their composition. However, RES addition decreased the indicated parameters.

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Section: Discussionmentioning

confidence: 99%

The protective effect of resveratrol against dentin bonding agents-induced cytotoxicity

Atalayın

Armağan

Konyalιoğlu

et al. 2015

Dental Materials Journal

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“…The S-phase accumulation is not counteracted by Trolox (vitamin E), indicating that an increased ROS level is not directly involved. In another study, HEMA induced significant DNA migration, detected by the comet assay, in human salivary glands as human target cells of carcinogenesis (Kleinsasser et al 2006), as previously reported in lymphocytes (Kleinsasser et al 2004). Both HEMA and methacrylic acid (MAA), HEMA hydrolysis HGFs, primary human gingival fibroblasts; ROS, reactive oxygen species; TNF a, tumour necrosis factor alpha; PGE 2 , prostaglandin 2; V794 fibroblasts, Chinese hamster fibroblast-like lung cells; RPC-C2A pulp cells, rat clonal dental pulp cells; NAC, N-acetylcysteine; PKC a, protein kinase C alpha isoform; iNOS, inducible nitric oxide synthase; Bisindolylmaleimide VIII, PKC a pharmacological inhibitor; RAW264.7, mouse leukaemic monocytes macrophage cell line; COX-2, inducible cyclooxygenase.…”

Section: Hema-induced Dna Damagementioning

confidence: 96%

HEMA‐induced cytotoxicity: oxidative stress, genotoxicity and apoptosis

2014

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Gallorini M, Cataldi A, di Giacomo V. HEMA-induced cytotoxicity: oxidative stress, genotoxicity and apoptosis.International Endodontic Journal, 47, 813-818, 2014. Dental resin composites consist of organic polymers with inorganic fillers used as bonding resins and direct filling materials in dentine adhesives and as sealing agents for inlays, crowns and orthodontic brackets. Despite various modifications in the formulation, the chemical composition of composite resins includes inorganic filler particles and additives, which are incorporated into a mixture of an organic resin matrix. Among them, 2-hydroxyethylmethacrylate (HEMA) is one of the most frequently used. Several studies have attempted to clarify the mechanisms underlying HEMA cytotoxicity. Most of them support the hypothesis that this compound, once released in the oral environment, increases reactive oxygen species (ROS) production and oxidative DNA damage through double-strand breaks evidenced by in vitro presence of micronuclei. As a consequence, the glutathione detoxifying intracellular pool forms adducts with HEMA through its cysteine motif and inflammation begins to occur: transcription of early genes of inflammation such as tumour necrosis factor a or inducible cyclooxygenase up to the secretion of prostaglandins 2. These phenomena are counteracted by N-acetylcysteine (NAC), a nonenzymatic antioxidant, but not by vitamin E or other antioxidant. Consequently, NAC prevents HEMA-induced apoptosis acting as a direct ROS scavenger. This minireview collects the most significant papers on HEMA and tries to make an overview of its cytotoxicity on different cell types and experimental models.

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“…tumorigenesis 33) . Several studies have reported genotoxic effects of TEGDMA and UDMA in different cell types using different assays 8,11,[38][39][40][41] . For example, Wisniewska-Jarosinska et al 8) used comet assays to show that both UDMA and TEGDMA induced genotoxicity when applied singly to Chinese hamster ovary cells, but a combination of both did not produce a significant increase in these effects.…”

Section: Genotoxicity Assaymentioning

confidence: 99%

Evaluation of residual monomer release and toxicity of self-adhesive resin cements

Kurt

Altıntaş

Kızıltaş

et al. 2018

Dental Materials Journal

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The aim of this study was to evaluate the amount of leached residual monomers from self-adhesive resin cements and evaluate their toxicity in-vitro. A total of 60 disk-shaped specimens (5 mm in diameter and 0.5 mm in thickness) were prepared from each cement (RelyX U200, SpeedCEM, G-Cem) (n=20). Specimens were immersed in artificial saliva and the amount of released monomers [urethane dimethacrylate (UDMA) and triethyleneglycol dimethacrylate (TEGDMA)] was identified. Then, the cytotoxicity and genotoxicity effect on cells were evaluated using the defined amounts of released monomers from cements. The highest monomer release was detected in G-Cem (p<0.05). The highest cytotoxicity value was identified from SpeedCEM (p<0.01) and the highest genotoxicity values were calculated from RelyX U200 (p<0.05). Released UDMA and TEGDMA from self-adhesive resin cements induced cytotoxicity and genotoxicity effect on cells.

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Cytotoxic and genotoxic effects of resin monomers in human salivary gland tissue and lymphocytes as assessed by the single cell microgel electrophoresis (Comet) assay

Cited by 135 publications

References 36 publications

The protective effect of resveratrol against dentin bonding agents-induced cytotoxicity

The protective effect of resveratrol against dentin bonding agents-induced cytotoxicity

HEMA‐induced cytotoxicity: oxidative stress, genotoxicity and apoptosis

Evaluation of residual monomer release and toxicity of self-adhesive resin cements

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