Cytotoxic and genotoxic effects of defence secretion of Ulomoides dermestoides on A549 cells

Crespo, Rosana; Villaverde, M. Luciana; Girotti, Juan Roberto; Güerci, Alba Mabel; Juárez, M. Patricia; Bravo, Margarita María García de

doi:10.1016/j.jep.2011.04.056

Cited by 40 publications

(32 citation statements)

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“…Plates were returned for incubation over the indicated time course (typically up to an additional 4 d). Dichloromethane extracts were prepared from laboratory-reared adults of T. castaneum, as previously described (26). The extracts contained mainly ethyl-and methyl-1,4-benzoquinones and 1-pentadecene, as verified by thin-layer chromatography.…”

Section: Methodsmentioning

confidence: 99%

“…The former is of particular importance as a worldwide pest of stored products, resulting in significant agricultural losses per year that often disproportionately affect developing nations (24,25). The latter, curiously enough, is consumed as an alternative medicine for the treatment of a range of illnesses including diabetes and cancer (26). The genome of T. castaneum is available, and it has become an important model system for insect development (27,28).…”

Section: Significancementioning

confidence: 99%

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Tenebrionid secretions and a fungal benzoquinone oxidoreductase form competing components of an arms race between a host and pathogen

Pedrini

Ortiz‐Urquiza

Huarte‐Bonnet

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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Entomopathogenic fungi and their insect hosts represent a model system for examining invertebrate-pathogen coevolutionary selection processes. Here we report the characterization of competing components of an arms race consisting of insect protective antimicrobial compounds and evolving fungal mechanisms of detoxification. The insect pathogenic fungus Beauveria bassiana has a remarkably wide host range; however, some insects are resistant to fungal infection. Among resistant insects is the tenebrionid beetle Tribolium castaneum that produces benzoquinone-containing defensive secretions. Reduced fungal germination and growth was seen in media containing T. castaneum dichloromethane extracts or synthetic benzoquinone. In response to benzoquinone exposure, the fungus expresses a 1,4-benzoquinone oxidoreductase, BbbqrA, induced >40-fold. Gene knockout mutants (ΔBbbqrA) showed increased growth inhibition, whereas B. bassiana overexpressing BbbqrA (Bb::BbbqrAO) displayed increased resistance to benzoquinone compared with wild type. Increased benzoquinone reductase activity was detected in wild-type cells exposed to benzoquinone and in the overexpression strain. Heterologous expression and purification of BbBqrA in Escherichia coli confirmed NAD(P)H-dependent benzoquinone reductase activity. The ΔBbbqrA strain showed decreased virulence toward T. castaneum, whereas overexpression of BbbqrA increased mortality versus T. castaneum. No change in virulence was seen for the ΔBbbqrA or Bb::BbbqrAO strains when tested against the greater wax moth Galleria mellonella or the beetle Sitophilus oryzae, neither of which produce significant amounts of cuticular quinones. The observation that artificial overexpression of BbbqrA results in increased virulence only toward quinone-secreting insects implies the lack of strong selection or current failure of B. bassiana to counteradapt to this particular host defense throughout evolution.

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Section: Methodsmentioning

confidence: 99%

Section: Significancementioning

confidence: 99%

Tenebrionid secretions and a fungal benzoquinone oxidoreductase form competing components of an arms race between a host and pathogen

Pedrini

Ortiz‐Urquiza

Huarte‐Bonnet

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

127

118

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“…In addition to being behaviorally aversive, pBQN is toxic to mammalian cells (Crespo et al, 2011;Fisher et al, 2011;Sarma et al, 2011;Tian et al, 2012). In humans, prolonged exposure to insect secretions of benzoquinone results in ulcers, blisters or discolored skin lesions with a local burning sensation (de Capitani et al, 2011), but the potential involvement of TRPA1 in these symptoms is unknown.…”

Section: Introductionmentioning

confidence: 99%

Benzoquinone Reveals a Cysteine-Dependent Desensitization Mechanism of TRPA1

Ibarra

Blair

2013

Molecular Pharmacology

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The transient receptor potential ankyrin 1 (TRPA1) nonselective cation channel has a conserved function as a noxious chemical sensor throughout much of Metazoa. Electrophilic chemicals activate both insect and vertebrate TRPA1 via covalent modification of cysteine residues in the amino-terminal region. Although naturally occurring electrophilic plant compounds, such as mustard oil and cinnamaldehyde, are TRPA1 agonists, it is unknown whether arthropod-produced electrophiles activate mammalian TRPA1. We characterized the effects of the electrophilic arthropod defensive compound para-benzoquinone (pBQN) on the human TRPA1 channel. We used whole-cell recordings of human embryonic kidney cells heterologously expressing either wild-type TRPA1 or TRPA1 with three serinesubstituted cysteines crucial for electrophile activation (C621S, C641S, C665S). We found that pBQN activates TRPA1 starting at 10 nM and peaking at 300 nM; higher concentrations caused rapid activation followed by a fast decline. Activation by pBQN required reactivity with cysteine residues, but ones that are distinct from those previously reported to be the key targets of electrophiles. The current reduction we found at higher pBQN concentrations was a cysteine-dependent desensitization of TRPA1, and did not require prior activation. The cysteines required for desensitization are not accessible to all electrophiles as iodoacetamide and internally applied 2-(trimethylammonium) ethyl methanesulfonate failed to cause desensitization (despite large activation). Interestingly, following pBQN desensitization, wild-type TRPA1 had dramatically reduced response to the nonelectrophile agonist carvacrol, whereas the triple cysteine mutant TRPA1 retained its full response. Our results suggest that modification of multiple cysteine residues by electrophilic compounds can generate both activation and desensitization of the TRPA1 channel.

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“…At some time, this practice was transferred by immigrants to Brazil and Argentina where the alternative treatment continues to the present day and is sometimes called coleoterapia (2). Articles have been published examining the effects of the extracts of U. dermestoides on inflammation and cell proliferation (3,4) We did not find any published reports of an association between traditional beetle therapy and eosinophilic pneumonia. Nevertheless, we made a diagnosis of beetle therapy-induced acute eosinophilic pneumonia in our patient based on the temporal relationship between the onset of his respiratory symptoms and ingestion of the beetles, coupled with peripheral eosinophilia and compatible lung radiographic infiltrates, and the prompt resolution of lung disease after he stopped consuming beetle larvae.…”

mentioning

confidence: 61%

Acute Eosinophilic Pneumonia Associated with Ingestion of Ulomoides dermestoides Larvae (“Chinese Beetles”)

Campion

Knox²

2014

Annals ATS

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A 66-year-old Hispanic man was evaluated in the Emergency Department for a 2-week history of nonproductive cough and shortness of breath. He reported intermittent experience of fever, but denied chills, fatigue, night sweats, purulent sputum, hemoptysis, orthopnea, paroxysmal nocturnal dyspnea, or chest pain. He had no recent exposure to others who were acutely ill and no recent travel or hospitalization.The man was known to have hypertension, diabetes mellitus, and coronary artery disease and had undergone a prostatectomy for treatment of prostate cancer. He was retired. He denied any significant animal or environment exposures except that he had repaired his washing machine about a month earlier and remembers inhaling dust at that time. He was raised in California and lived subsequently for several decades in Arizona. There was no history of allergies or asthma. His medications were aspirin, amlodipine, metoprolol, ezetimibe, sitagliptin, metformin, and insulin. He was prescribed azithromycin 1 week earlier with no improvement in his respiratory symptoms.In the Emergency Department, the man's temperature was 98.2 8 F, heart rate 104 beats/min, and blood pressure 107/56 mm Hg. By pulse oximetry, his arterial oxygen saturation was 94% while he breathed supplemental oxygen at 2 L/min via nasal cannula. He was alert, articulate, and appeared in good health. There was no cervical lymphadenopathy, oral lesions, or ulcers. He appeared mildly tachypneic. Auscultation of the chest revealed rhonchi, which were most pronounced in the right upper lobe, but no wheezing. Auscultation of his heart disclosed a regular heart rhythm with III/VI aortic systolic murmur and normal S1 and S2. There was no peripheral edema, skin rash, or joint swelling. No motor or sensory abnormality was noted.The white blood cell count was 24.8 1,000/mL with 28% eosinophils and 42% neutrophils. The hemoglobin was 14 g/dL, and the platelet count was 254 1,000/mL. The serum sodium was 138 mmol/L, potassium 4.3 mmol/L, chloride 102 mmol/L, bicarbonate 22 mmol/L, BUN 14 mg/dL, creatinine 0.7 mg/dL, bilirubin 0.4 mg/dL, AST 17 IU/L, ALT 15 IU/L, alkaline phosphatase 117 IU/L, serum calcium 9.7 mg/dL, and serum glucose 175 mg/dL.A chest radiograph revealed ill-defined bilateral upper lobe hazy opacities. Computerized tomographic (CT) imaging of the chest showed multilobar opacities with bilateral upper lobe ground glass infiltrates and peripheral areas of consolidation (Figure 1).The man was admitted to the hospital and was treated with empiric intravenous vancomycin and piperacillin/tazobactam. All of the following tests were negative or normal: blood culture, sputum culture, serum coccidioidomycosis antibodies, 1-3 b-D-glucan, Aspergillus galactomannan antibodies, Strongyloides antibodies, stool for parasite testing, Legionella urinary antigen, and HIV and autoimmune screening tests.Bronchoscopy was performed. A sample of fluid obtained by bronchoalveolar lavage of the right upper lobe revealed a leukocyte count of 1,782/mL with 4% neutrophils, 6% lymp...

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Cytotoxic and genotoxic effects of defence secretion of Ulomoides dermestoides on A549 cells

Cited by 40 publications

References 24 publications

Tenebrionid secretions and a fungal benzoquinone oxidoreductase form competing components of an arms race between a host and pathogen

Tenebrionid secretions and a fungal benzoquinone oxidoreductase form competing components of an arms race between a host and pathogen

Benzoquinone Reveals a Cysteine-Dependent Desensitization Mechanism of TRPA1

Acute Eosinophilic Pneumonia Associated with Ingestion of Ulomoides dermestoides Larvae (“Chinese Beetles”)

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