Cytotoxic and genotoxic effects of silver nanoparticles on meristematic cells of Allium cepa roots: A close analysis of particle size dependence

Scherer, Marisa D.; Spósito, Juliana Caroline Vivian; Falco, William F.; Grisolia, Alexéia Barufatti; Andrade, L.H.C.; Lima, S.M.; Machado, Giovanna; Nascimento, Valter Aragão do; Gonçalves, Daniel A.; Wender, Heberton; Oliveira, S. L.; Caires, Anderson R. L.

doi:10.1016/j.scitotenv.2018.12.444

Cited by 120 publications

(41 citation statements)

References 66 publications

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“…Therefore, the Ag + liberation in distilled water was diameter-dependent as a result of the larger total surface areas of the smaller AgNPs (Wang et al, 2014). These data are in accordance with previous studies that observed that the AgNPs-PVP are stable in aqueous solution, releasing less than 1% of Ag + species (Cvjetko et al, 2017;Nallanthighal et al, 2017;Scherer et al, 2019). PVP strongly binds to the nanoparticles surface avoiding aggregation (Nallanthighal et al, 2017).…”

Section: Agnps Characterizationsupporting

confidence: 90%

“…(Nallanthighal et al, 2017). As recently demonstrated in a paper published by our research group, Ag ions play as a minor contributor to the toxicity of the PVP-coated AgNPs when released at concentrations lower than 1% (Scherer et al, 2019). Consequently, a minimal contribution of Ag + toxicity is expected.…”

Section: Discussionsupporting

confidence: 53%

“…Here, Vicia faba plants were chosen for investigating the physiological changes induced by AgNPs with different diameters and bulk silver (AgBulk) at a later stage of plant growth. Thus, the present study reports the toxicity dependence of AgBulk (micrometer-size particles) and AgNPs with three different diameters (20, 51, and 73 nm) when internalized in Vicia faba leaves using a wellestablished toxic nanoparticle concentration of 100 mg L -1 (Kumari et al, 2009;Scherer et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

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Phytotoxicity of silver nanoparticles on Vicia faba: Evaluation of particle size effects on photosynthetic performance and leaf gas exchange

Falco

Scherer

Oliveira

et al. 2020

Science of The Total Environment

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Section: Agnps Characterizationsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 53%

Section: Introductionmentioning

confidence: 99%

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Phytotoxicity of silver nanoparticles on Vicia faba: Evaluation of particle size effects on photosynthetic performance and leaf gas exchange

Falco

Scherer

Oliveira

et al. 2020

Science of The Total Environment

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“…Cox et al (2016) reported that AgNPs were more toxic than TiO 2 , resulting in higher oxidative stress and genotoxicity, as well as lower seed germination and root elongation in Arabidopsis thaliana (L.) Heynh., Oryza sativa L., Lemna gibba L., Ricinus communis L., Vigna radiate (L.) R. Wilczek, Cucurbita pepo L. Exposure to >25 mg kg −1 of AgNPs and Ag + induces oxidative stress, similar to other trace elements (Kumari et al, 2009). The toxicity of AgNPs is size dependent, with smaller AgNPs (<20 nm in diameter) being more penetrative and toxic than larger particles (Scherer et al, 2019).…”

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confidence: 99%

The Mobility of Silver Nanoparticles and Silver Ions in the Soil‐Plant System

et al. 2019

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The widespread use of silver nanoparticles (AgNPs) as a bactericide will ultimately result in their increased concentration in soils. We sought to determine the likely mobility, toxicity, and plant uptake of Ag applied to soil as either AgNPs or Ag + . We measured the solubility, toxicity, and plant uptake of both AgNPs and Ag + in an immature Pallic soil, a Templeton loamy silt (pH = 5.1), and a granular silt loam (pH = 6.0). The sorption of AgNPs by the test soils was significantly greater than Ag + , and both moieties were more strongly sorbed at lower concentrations and higher pH values. Between pH 4 and 8, distribution coefficient (K D ) values increased from <10 up to ?500 L kg −1 for Ag + , and from 100 to 10,000 L kg −1 for AgNPs. There was strong evidence that our citrate-coated AgNPs were transformed into Ag + during the course of the plant growth experiments, and plant responses were similar for both the Ag + and AgNP treatments. Soil concentrations >100 mg kg −1 significantly reduced the biomass of Lolium perenne L. and resulted in foliar concentrations of up to 10 mg kg −1 dry matter. At a soil concentration of 70 mg kg −1 , silverbeet [Beta vulgaris L. ssp. maritima (L.) Arcang.] and spinach (Spinacia oleracea L.) accumulated 5 to 10 mg Ag kg −1 , which may present a human health risk. Regarding citrate-coated AgNPs, the environmental impact of release is largely determined by the equivalent mass concentration of Ag + , into which they will ultimately transform. Given the widespread interest in AgNPs, there is limited knowledge on the behavior of Ag + in soil and this should be the subject of future research.

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“…Several methodologies can be used to characterize particle size, such as transmission electron microscopy (TEM) [18,19], scanning electron microscopy (SEM) [20,21], atomic force microscopy (AFM) [22], visible ultra violet spectroscopy (UV-Vis), analytical ultracentrifugation (AUC) [23,24], flow field fractionation (FFF) [25], X-ray diffraction (XRD) [26], and light dynamic scattering (LDS) [27]. Nevertheless, most of these methods require careful sample preparation (TEM, SEM, and AFM), whereas AFM, AUC, and FFF have the possibility of presenting artifacts as well as have high cost such as AFM [28][29][30].…”

Section: Introductionmentioning

confidence: 99%

Multivariate Model Based on UV-Vis Spectroscopy and Regression in Partial Least Squares for Determination of Diameter and Polydispersity of Silver Nanoparticles in Colloidal Suspensions

Rodrigues

Oliveira

et al. 2020

Journal of Nanomaterials

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In the universe of nanomaterials, silver nanoparticles (AgNPs) have attracted the attention of researchers because of their optical, catalytic, antimicrobial, fungicidal, and bactericidal properties. Recently, studies have correlated the toxicity and efficacy of antimicrobial activity with surface-volume ratio, morphology, polydispersity, ligand types, particle size, and stability of AgNPs. Soon, the need for characterization of properties such as diameter and polydispersity is clear. The methodologies conventionally used for characterization of AgNPs, although accurate, are generally expensive and laborious and can degrade the sample. Thus, the development of methodologies based on UV-Vis spectroscopy and chemometric techniques appears as an alternative for the characterization of diameter and polydispersity of the nanoparticles. For the development of the methodology in question, 50 samples were synthesized, varying the type, volume, and concentration of the reagents in order to increase the diameter and polydispersity values. All samples were analyzed by DLS and UV-Vis spectroscopy. For the construction of multivariate calibration models, the calibration and validation sets were selected using the SPXY algorithm, and their predictive capacity was evaluated based on the method figures. The model that presented the best predictive capacity was the one built with the pretreated spectra with the 1st derivative with a 15-point window and 2nd-order polynomial, providing prediction errors of 5.31% and 4.43% for diameter and polydispersity, respectively.

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Cytotoxic and genotoxic effects of silver nanoparticles on meristematic cells of Allium cepa roots: A close analysis of particle size dependence

Cited by 120 publications

References 66 publications

Phytotoxicity of silver nanoparticles on Vicia faba: Evaluation of particle size effects on photosynthetic performance and leaf gas exchange

Phytotoxicity of silver nanoparticles on Vicia faba: Evaluation of particle size effects on photosynthetic performance and leaf gas exchange

The Mobility of Silver Nanoparticles and Silver Ions in the Soil‐Plant System

Multivariate Model Based on UV-Vis Spectroscopy and Regression in Partial Least Squares for Determination of Diameter and Polydispersity of Silver Nanoparticles in Colloidal Suspensions

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