Cytotoxic and other bioactivities of a novel and known sesquiterpene lactones isolated from Vernonia leopoldi (Sch. Bip. ex Walp.) Vatke in breast cancer cell lines

Tuasha, Nigatu; Escobar, Zilma; Seifu, Daniel; Gadisa, Endalamaw; Petros, Beyene; Sterner, Olov; Oredsson, Stina

doi:10.1016/j.toxrep.2022.02.011

Cited by 6 publications

(12 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Taxonomic identification was done by expert botanist Ms. Lucy M. Wambui and taxonomist Mr. Patrick B. Mutiso of the University of Nairobi, and a voucher specimen (TEREFE E. /044) was deposited at the USIU herbarium for future reference. The extraction and isolation of the plant was carried out as previously described [ 2 , 18 , 19 ].…”

Section: Methodsmentioning

confidence: 99%

“…Compounds were isolated as previously described [ 2 , 18 , 19 ]. Briefly, 5 kg of air-dried stem bark of C. megalocarpus was extracted using 1:1 CH 2 Cl 2 :CH 3 OH by standing at room temperature, to give 0.33 kg of a solid.…”

Section: Methodsmentioning

confidence: 99%

“…The cytotoxicity test was conducted to evaluate the cytotoxicity of the plant extracts by measuring cell death caused by the plant extracts. The assay was conducted using an MTT colorimetric assay as described by [ 2 , 19 , 31 , 35 , 36 ]. The MTT assay is based on the reduction of the yellow-colored tetrazolium salt MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) by NAD(P)H-dependent cellular oxidoreductase enzymes [ 37 ] to an insoluble dark-blue colored formazan that can be measured spectrophotometrically [ 38 ].…”

Section: Methodsmentioning

confidence: 99%

“…CPE induced by the virus was observed under a microscope after 3–4 days. The TCID 50 was then calculated as described by [ 2 , 19 ]. For the anti-HIV activity testing, 640 µL of HIV-1 IIIB virus at 1.26 × 10 8 TCID 50 /mL was used to infect 1 × 10 8 cell/mL.…”

Section: Methodsmentioning

confidence: 99%

“…Sesquiterpene lactone is a large subclass of terpenoids often found in plants. Sesquiterpene lactones play an important role in plant defence in nature, acting as antibacterials, antivirals, antifungals, and insecticides [ 19 ] and have piqued the interest of researchers in recent years, primarily due to their cytotoxic and anticancer properties [ 20 ]. Several eudesmane-type sesquiterpene lactones have displayed cytotoxic and potential antiviral activity.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Anti-HIV Ermiasolides from Croton megalocarpus

et al. 2022

View full text Add to dashboard Cite

In recent years, elucidation of novel anti-HIV bioactive compounds from natural products is gaining importance rapidly, not only from the research and publications, but also from controlled clinical studies. Here we report three new anti-HIV eudesmane-type sesquiterpenes, 5β-Hydroxy-8α-methoxy eudesm-7(11)-en-12,8-olide (1), 5β,8α-Dihydroxy eudesm-7(11)-en-12,8-olide (2) and 5β-Hydroxy-8H-β-eudesm-7(11)-en-12,8-olide (3). These are trivially named ermiasolide A-C and were isolated from the bark of Croton megalocarpus. 5β-Hydroxy-8α-methoxy eudesm-7(11)-en-12,8-olide (1), showed the highest anti-HIV activity by inhibiting 93% of the viral replication with an IC50 = 0.002 µg/mL. On the other hand, 5β-Hydroxy-8H-β-eudesm-7(11)-en-12,8-olide (3) and 5β,8α-dihydroxy eudesm-7(11)-en-12,8-olide (2), inhibited viral replication by 77.5% at IC50 = 0.04 µg/mL and 69.5% at IC50 = 0.002 µg/mL, respectively. Molecular docking studies showed that the proposed mechanism of action leading to these results is through the inhibition of HIV-protease.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Anti-HIV Ermiasolides from Croton megalocarpus

et al. 2022

View full text Add to dashboard Cite

show abstract

Advances in detecting fruit aroma compounds by combining chromatography and spectrometry

et al. 2023

View full text Add to dashboard Cite

Fruit aroma is produced by volatile compounds, which can significantly enhance fruit flavor. These compounds are highly complex and have remarkable pharmacological effects. The synthesis, concentration, type, and quantity of fruit aroma substances are affected by various factors, both abiotic and biotic. To fully understand the aroma substances of various fruits and their influencing factors, detection technology can be used. Many methods exist for detecting aroma compounds, and approaches combining multiple instruments are widely used. This review describes and compares each detection technology and discusses the potential use of combined technologies to provide a comprehensive understanding of fruit aroma compounds and the factors influencing their synthesis. These results can inform the development and utilization of fruit aroma substances.

show abstract

Ethnobotanical study of endemic and non-endemic medicinal plants used by indigenous people in environs of Gullele botanical garden Addis Ababa, central Ethiopia: A major focus on Asteraceae family

et al. 2022

View full text Add to dashboard Cite

Gullele Botanical Garden (GBG) in Addis Ababa, Ethiopia is a joint venture of Addis Ababa government and the university. The garden has been built mainly to conserve the endemic plants and to advance the research on the endemic and non-endemic plants collected from different part of Ethiopia. Many traditional healers from the environs of GBG and different subcities of Addis Ababa depend on the garden for their practice of traditional medicine but there is no systematic documentation of the traditional medicinal knowledge of these healers. The main objective of the present study is to comprehensively document the ethnobotanical and ethnomedicinal information from the traditional healers of different ethnic and cultural groups depending on GBG and to create a database of the endemic plants used by these healers. The ethnobotanical and ethnomedicinal data obtained from 60 traditional healers have been analyzed both qualitatively and quantitatively. A total of 81 medicinal plants belonging to 47 families have been identified. Majority of the plants used belonged to Asteraceae (12) family. The most frequently used plant form and plant parts are herbs and leaves. The major method adopted by the healers for preparation and administration of traditional medicine is crushing and topical, respectively. Skin and general diseases are the most important ailments treated by the healers. The three most cited plants used to treat diseases are Echinops kebericho Mesfin (60), Hagenia abyssinica (Bruce) J.F.Gmel (60) and Laggera tomentosa (A.Rich.) Sch.Bip. ex Oliv. & Hiern (58). The present study is the first systematic, qualitative, and quantitative ethnobotanical analysis and documentation done on the use of the medicinal plants from GBG for traditional medicine. In addition, our study reveals that E. kebericho is endemic and endangered plant and is highly used in traditional medicine. Therefore, GBG authorities should take steps for the propagation and restoration of this plant. Further it is suggested that the pharmacological properties of the roots and leaves of E. kebericho should be compared to find the possibility of use of leaves in place of roots for the preparation of traditional medicine which would help in conserving this endemic plant of Ethiopia.

show abstract

Cytotoxic and other bioactivities of a novel and known sesquiterpene lactones isolated from Vernonia leopoldi (Sch. Bip. ex Walp.) Vatke in breast cancer cell lines

Cited by 6 publications

References 34 publications

Anti-HIV Ermiasolides from Croton megalocarpus

Anti-HIV Ermiasolides from Croton megalocarpus

Advances in detecting fruit aroma compounds by combining chromatography and spectrometry

Ethnobotanical study of endemic and non-endemic medicinal plants used by indigenous people in environs of Gullele botanical garden Addis Ababa, central Ethiopia: A major focus on Asteraceae family

Contact Info

Product

Resources

About