Cytotoxic, Anti-bacterial, and Wound-healing Activity of Prenylated Phenols from the Kurdish Traditional Medicinal Plant Onobrychis Carduchorum (Fabaceae)

Abstract

Onobrychis carduchorum (Fabaceae) is a plant widely employed in Kurdish traditional medicine to cure wounds, inflammations, and other skin diseases. We could isolate ten different polyphenols from the acetone extract of this plant: 1–4 are isoflavones, having a genistein skeleton; 5–7 are flavanones, having a naringenin skeleton; and 8–10 are prenylated dihydro-stilbenes. In particular, 8–10 have been isolated, so far, only from Glycyrrhiza glabra (liquorice). Many of the above prenylated phenols showed significant toxicity on some human breast cancer cell lines, and a relevant growth inhibition of Staphylococcus aureus strains. In addition, 9 and 10 had marked wound healing activity. It is suggested that these bioactivities are responsible, at least partly, for the plant’s traditional use.

Potential use of wild Onobrychis species for climate change mitigation and adaptation

“…leaves compresses are traditionally used in Turkey as a styptic and to cure wounds, inflammations, and other skin diseases (Dalar et al., 2018; Mükemre et al., 2015). This ethnical use of O. carduchorum was supported by the abundant presence in its leaves of prenylated polyphenols with antibacterial and wound healing activities, along with radical scavenging ability (Clericuzio et al., 2020). Ten different polyphenols were extracted from O. carduchorum including four isoflavones having a genistein skeleton, three flavanones having a naringenin skeleton and three prenylated dihydro‐stilbenes.…”

“…Ten different polyphenols were extracted from O. carduchorum including four isoflavones having a genistein skeleton, three flavanones having a naringenin skeleton and three prenylated dihydro‐stilbenes. In particular, prenylated dihydro‐stilbenes were isolated before, so far, only from Glycyrrhiza glabra L. Many of the prenylated phenols isolated from O. carduchorum showed significant cytotoxicity on some human breast cancer cell lines, and a relevant growth inhibition of Staphylococcus aureus strains suggesting that these bioactivities are responsible for the plant's traditional use (Clericuzio et al., 2020). In this sense, aerial parts of different Onobrychis species from Turkey, such as O. oxyodonta arial parts ethanolic extracts, O. armena Boiss & Huet methanolic flower and roots extracts, show antioxidant activity, moderate antitumor activity, and antimicrobial activity against some bacteria, fungus and fish pathogens (Karakoca et al., 2015; Usta et al., 2014).…”

In vitro Evaluation of Skin Related Enzyme Inhibitory Effect and Emulgel Formulation Development Studies of Onobrychis Argyrea subsp. Argyrea with Phytochemical Analysis

“…For instance, there are records that Onobrychis carduchorum, Onobrychis altissima were used against wound, inflammations, cut and other skin diseases. [2,3,4] Also, biological activity studies on O. hypargyrea and O. argyrea subsp. isaurica was demon-strated their anti-tyrosinase activity for hyperpigmentation.…”

“…Onobrychis species have been used in folk medicine for various skin illnesses. For instance, there are records that Onobrychis carduchorum , Onobrychis altissima were used against wound, inflammations, cut and other skin diseases [2,3,4] . Also, biological activity studies on O. hypargyrea and O. argyrea subsp.…”

“…results were expressed as the mean of triplicates � standard deviation (S.D.) and as mg butylated hydroxyl toluene equivalents (BHTE) in 1 g sample 2. results were expressed as the mean of triplicates � standard deviation (S.D.)…”