Cytotoxic, Anti-Proliferative and Apoptotic Effects of Tribulus terrestris L. Fruit Extract on Human Prostate Cancer Lncap and Colon Cancer HT-29 Cell Lines

Pourali, Masoud; Yaghoobi, Mohammad; Sormaghi, M H Salehi

doi:10.5812/jjnpp.33561

Cited by 6 publications

(6 citation statements)

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“…In this regard, 250 and 500 mg/kg of TT extract by improving antioxidant and anti-apoptotic pathways led to an improvement in the cardiac function of rats with cardiac ischemia, although in some markers, a higher dose had more favorable effects (11). The researchers found that 85 and 50 mg/kg of hydroalcoholic extract of TT significantly improved lipid profile in rats with coronary heart disease induced by injecting isoproterenol (13). Moreover, 0.3, 7.1, and 8.7 µg/mL of TT significantly reduced TNFL and FasL levels in the cell and induced apoptosis in cancer cells (12).…”

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confidence: 99%

“…TT with its antioxidant and anti-inflammatory effects improves fat metabolism and appears to inhibit inflammatory and apoptotic activities of tumor necrosis factor-alpha ligand (TNFL) and FasL as well as cell death (13). In this regard, 250 and 500 mg/kg of TT extract by improving antioxidant and anti-apoptotic pathways led to an improvement in the cardiac function of rats with cardiac ischemia, although in some markers, a higher dose had more favorable effects (11).…”

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confidence: 99%

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Effect of Eight Weeks of Resistance Training and Consumption of Tribulus Terrestris on Androgenic Receptor-1, Fas Ligand Gene Expression, and Lipid Profiles in Rats Exposed to Stanozolol

Derakhshandeh

Taghian

Dehkordi

et al. 2020

Avicenna J Med Biochem

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Background: Protective effect of medicinal plants on the heart has been reported, but the effect of resistance training (RT) and Tribulus terrestris (TT) on the heart exposed to anabolic-androgenic steroids (AAS) abuse is still unknown. Objectives: The present study aimed to investigate the effect of RT and TT on androgen receptor-1 (ar-1), Fas ligand (fasl) gene expression and lipid profiles in rats exposed to stanozolol (S). Methods: Thirty-five male rats were selected and divided into 7 groups as follows: (1) sham (normal saline/Sh), (2) stanozolol (S), (3) S+100 mg/kg TT (S+TT100), (4) S+ 50 mg/kg TT (S+TT50), (5) S+RT+TT, (6) S+RT+TT100, and (7) S+RT+TT50. Over a course of eight-week period, groups 3, 4, 6, and 7 received 50 and 100 mg/kg/d doses of TT peritoneally and groups 5-7 performed three sessions of increasing RT per week. Results: RT decreased plasma cholesterol and low-density lipoprotein cholesterol (LDL-C) levels, as well as ar-1 and fasl gene expression in S-exposed rats (P<0.05). TT50, TT100, SRTT100, and SRTT50 reduced ar-1 and fasl gene expressions (P<0.05). TT50 reduced triglyceride (TG), cholesterol and increased high-density lipoprotein-cholesterol (HDL-C) (P≤0.01), and TT100 decreased LDL-C levels (P<0.05). Additionally, SRTT100 reduced TG, cholesterol, and LDL-C levels and increased HDL-C level (P<0.05), and SRTT50 decreased cholesterol level and increased HDL-C level in S-exposed rats (P<0.05). Conclusion: RT and consumption of TT appear to have protective effects on the improvement of apoptosisdependent androgen receptor-1 and lipid profile in S-exposed rats.

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confidence: 99%

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confidence: 99%

Effect of Eight Weeks of Resistance Training and Consumption of Tribulus Terrestris on Androgenic Receptor-1, Fas Ligand Gene Expression, and Lipid Profiles in Rats Exposed to Stanozolol

Derakhshandeh

Taghian

Dehkordi

et al. 2020

Avicenna J Med Biochem

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“…A previous report on T. terrestris extract showed cytotoxic effects on fibroblast cell lines [75]. Moreover, Pourali M, et al, found higher cytotoxicity in colon and prostate cancer cell line assays than in normal fibroblast cell lines [74].…”

Section: Discussionmentioning

confidence: 84%

“…The IC 50 values of 179.62 and 189.70 µg/mL were obtained with methanol and aqueous extract, respectively. Similarly, studies also reported the cytotoxicity activity of alcoholic, hydroalcoholic, and aqueous extracts of T. terrestris [72][73][74]. It was observed that T. terrestris extract does not have much cytotoxic effect on normal cell lines.…”

Section: Discussionmentioning

confidence: 95%

Tribulus terrestris Cytotoxicity against Breast Cancer MCF-7 and Lung Cancer A549 Cell Lines Is Mediated via Activation of Apoptosis, Caspase-3, DNA Degradation, and Suppressing Bcl-2 Activity

et al. 2022

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The primary objective of this research was to use flow cytometry to gain mechanistic insights into the cytotoxic effects of Tribulus terrestris extracts on breast cancer (MCF7) and lung cancer (A549) cell lines. T. terrestris was extracted using a Soxhlet apparatus in a progressive process. GC–MS was used to establish the phytochemical constituents. The amounts of phenolic compounds and flavonoids in the plant extracts were calculated using spectrophotometric analysis. The cytotoxicity of plant extracts was initially evaluated in non-malignant L929 cells, then in carcinogenic MCF-7 and A549 cell lines. Then, we performed an Annexin V assay, an anti-Bcl-2 assay, a Caspase-3 assay, and a DNA fragmentation (TUNEL) assay, using flow cytometry to investigate the underlying molecular processes. Based on the data, the methanolic extract of T. terrestris contained the highest amounts of phenolic compounds and flavonoids, with values of 169.87 µg GAE/g dwt and 160.12 µg QE/g dwt, respectively. Analysis by GC–MS revealed the presence of bioactive phytochemicals with proven cytotoxicity. Based on the MTT experiment, we determined that the IC50 values for the methanol extract’s effect on the viability of the MCF-7 and A549 cell lines were 218.19 and 179.62 µg/mL, respectively. The aqueous and methanol extracts were less cytotoxic when tested against the cancer-free L929 cell line (IC50 = 224.35 µg/mL). In both breast and lung cancer cells, the methanolic extract was found to activate caspase-3 and inhibit the Bcl-2 protein, resulting in early and late apoptosis and cell death via DNA damage. These findings point to cytotoxic effects of T. terrestris methanol extract against breast and lung cancer cell lines. Due to its potential as a source of anti-cancer chemotherapeutic medicines, T. terrestris warrants further investigation.

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“…While colon cancer cell viability was 10 % at 0.5 ppm, no cytotoxicity was observed on prostate cancer. At 12 ppm, the viability of prostate and cancer cells was reported as 10 % and 30 %, respectively [25]. In addition, the aqueous extracts of this plant obtained by ultrasonic wave assisted extraction and these extract's cytotoxicity was investigated on Hepa 1c1c7 and Ovcar 3 cells.…”

Section: Mtt Assaymentioning

confidence: 99%

Evaluation of the Cytotoxic Effects of Ultrasonic Extracts of Tribulus Terrestris L. on MCF-7 Cell Line by MTT Assay

Akbaba

Öztürkkan

Sertçelik

2021

Hittite Journal of Science and Engineering

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I t is known that human beings used only natural medicines until 1800 in the treatment of diseases. Compounds obtained through isolation from natural sources since the beginning of 1800 have been synthesized in the laboratory environment since 1830 [1]. In recent years, the belief that natural remedies are safer than synthetic drugs have led to an increase in people's use of natural products such as herbs, phytotherapeutics and phytopharmaceuticals. Therefore, researchers conduct various studies on the biological significance of plant extracts [2, 3]. However, there is no regulatory system that determines the safety and required dose of natural products, or even commercially sells them. The most widely used of these natural products are herbs. Unfortunately, most of those who use these herbs therapeutically do not have adequate knowledge or training in the safe use of the products. For these reasons, many studies are required to standardize natural plant products and evaluate possible risks such as undesirable side effects, overdose and toxicity [4].Tribulus terrestris L. is known in Turkey as "deve çökerten", "çoban çökerten" and "çarık dikeni". These names were given to the plant because its fruit has horn-shaped spikes (Figure 1

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Cytotoxic, Anti-Proliferative and Apoptotic Effects of Tribulus terrestris L. Fruit Extract on Human Prostate Cancer Lncap and Colon Cancer HT-29 Cell Lines

Abstract: Background: The medicinal herb Tribulus terrestris L. (Zygophyllaceae) has been used for a long time to treat various kinds of diseases including hepatocellular carcinoma.

Cited by 6 publications

References 5 publications

Effect of Eight Weeks of Resistance Training and Consumption of Tribulus Terrestris on Androgenic Receptor-1, Fas Ligand Gene Expression, and Lipid Profiles in Rats Exposed to Stanozolol

Effect of Eight Weeks of Resistance Training and Consumption of Tribulus Terrestris on Androgenic Receptor-1, Fas Ligand Gene Expression, and Lipid Profiles in Rats Exposed to Stanozolol

Tribulus terrestris Cytotoxicity against Breast Cancer MCF-7 and Lung Cancer A549 Cell Lines Is Mediated via Activation of Apoptosis, Caspase-3, DNA Degradation, and Suppressing Bcl-2 Activity

Evaluation of the Cytotoxic Effects of Ultrasonic Extracts of Tribulus Terrestris L. on MCF-7 Cell Line by MTT Assay

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